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ATG7 Monoclonal Antibody (2B5A10)

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Antibody Testing Data (5)

ATG7 Antibody in Immunocytochemistry (ICC/IF)

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ATG7 Antibody (67341-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

ATG7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

67341-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

1:100

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2B5A10

Immunogen

ATG7 Fusion Protein Ag29172

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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DANCR promotes glioma cell autophagy and proliferation via the miR‑33b/DLX6/ATG7 axis.

Oncology reports

Yu W,Ma L,Li X

Wed Feb 01 00:00:00 EST 2023

TGF-β-induced PI3K/AKT/mTOR pathway controls myofibroblast differentiation and secretory phenotype of valvular interstitial cells through the modulation of cellular senescence in a naturally occurring in vitro canine model of myxomatous mitral valve disease.

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Tang Q,Markby GR,MacNair AJ,Tang K,Tkacz M,Parys M,Phadwal K,MacRae VE,Corcoran BM

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Thu Jun 01 00:00:00 EDT 2023

Rift Valley Fever Virus Nucleoprotein Triggers Autophagy to Dampen Antiviral Innate Immune Responses.

Journal of virology

Zhu X,Guan Z,Fang Y,Zhang Y,Guan Z,Li S,Peng K

Thu Apr 27 00:00:00 EDT 2023

Stanniocalcin2 inhibits the epithelial-mesenchymal transition and invasion of trophoblasts via activation of autophagy under high-glucose conditions.

Molecular and cellular endocrinology

Lai R,Ji L,Zhang X,Xu Y,Zhong Y,Chen L,Hu H,Wang L

Sun May 01 00:00:00 EDT 2022

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells.

International journal of molecular sciences

Pei T,Su G,Yang J,Gao W,Yang X,Zhang Y,Ren J,Shen Y,Liu X

Sun Dec 11 00:00:00 EST 2022

Fluid Shear Stress Regulates Osteogenic Differentiation via AnnexinA6-Mediated Autophagy in MC3T3-E1 Cells.

International journal of molecular sciences

Pei T,Su G,Yang J,Gao W,Yang X,Zhang Y,Ren J,Shen Y,Liu X

Sun Dec 11 00:00:00 EST 2022

High glucose and palmitic acid induces neuronal senescence by NRSF/REST elevation and the subsequent mTOR-related autophagy suppression.

Molecular brain

Xue WJ,He CF,Zhou RY,Xu XD,Xiang LX,Wang JT,Wang XR,Zhou HG,Guo JC

Mon Jul 18 00:00:00 EDT 2022

Purple sweet potato delphinidin-3-rutin represses glioma proliferation by inducing miR-20b-5p/Atg7-dependent cytostatic autophagy.

Molecular therapy oncolytics

Wang M,Liu K,Bu H,Cong H,Dong G,Xu N,Li C,Zhao Y,Jiang F,Zhang Y,Yuan B,Li R,Jiang J

Thu Sep 15 00:00:00 EDT 2022

Polyvinylpyrrolidone-Modified Taxifolin Liposomes Promote Liver Repair by Modulating Autophagy to Inhibit Activation of the TLR4/NF-κB Signaling Pathway.

Frontiers in bioengineering and biotechnology

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Stem cell research & therapy

Zhou J,Yang J,Dong Y,Shi Y,Zhu E,Yuan H,Li X,Wang B

Tue Jun 28 00:00:00 EDT 2022

Trillin inhibits myoblast differentiation via increasing autophagy.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Dai W,Liu K,Li R,Cao Y,Shen M,Tao J,Liu H

Sun May 01 00:00:00 EDT 2022

Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway.

Journal of cellular physiology

Guo J,Nie J,Chen Z,Wang X,Hu H,Xu J,Lu J,Ma L,Ji H,Yuan J,Xu B

Sat Oct 01 00:00:00 EDT 2022

Cordycepin inhibits cell senescence by ameliorating lysosomal dysfunction and inducing autophagy through the AMPK and mTOR-p70S6K pathway.

FEBS open bio

Zuo SQ,Li C,Liu YL,Tan YH,Wan X,Xu T,Li Q,Wang L,Wu YL,Deng FM,Tang B

Fri Oct 01 00:00:00 EDT 2021

Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.

Cancers

Dannheisig DP,Schimansky A,Donow C,Pfister AS

Fri Dec 10 00:00:00 EST 2021

Latcripin-7A from Lentinula edodes C_91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling.

European journal of pharmacology

Din SRU,Nisar MA,Ramzan MN,Saleem MZ,Ghayas H,Ahmad B,Batool S,Kifayat K,Guo X,Huang M,Zhong M

Wed Sep 15 00:00:00 EDT 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 EDT 2021

Combination effect of curcumin with docetaxel on the PI3K/AKT/mTOR pathway to induce autophagy and apoptosis in esophageal squamous cell carcinoma.

American journal of translational research

Deng L,Wu X,Zhu X,Yu Z,Liu Z,Wang J,Zheng Y

Wed Feb 03 00:00:00 EST 2021

SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction.

Frontiers in physiology

Man W,Gu J,Wang B,Zhang M,Hu J,Lin J,Sun D,Xiong Z,Gu X,Hao K,Guo B,Wei G,Zhang L,Song R,Li C,Wang H,Sun D

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Molecular cancer

Zhou C,Yi C,Yi Y,Qin W,Yan Y,Dong X,Zhang X,Huang Y,Zhang R,Wei J,Ali DW,Michalak M,Chen XZ,Tang J

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Molecular medicine reports

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Tue Sep 01 00:00:00 EDT 2020

Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling.

Frontiers in pharmacology

Xu Q,Fan Y,Loor JJ,Liang Y,Sun X,Jia H,Zhao C,Xu C

Thu Dec 31 00:00:00 EST 2020

Latcripin-7A, derivative of Lentinula edodes C_91-3, reduces migration and induces apoptosis, autophagy, and cell cycle arrest at G₁ phase in breast cancer cells.

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Tue Dec 01 00:00:00 EST 2020

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Bioinformatics

Protein Aliases: APG7-like; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; autophagy related 7 homolog; autophagy-related 7; Autophagy-related protein 7; DKFZp434N0735; hAGP7; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7

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Gene Aliases: 1810013K23Rik; Agp7; APG7-LIKE; APG7L; ATG7; Atg7l; Gm21553; GSA7

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UniProt ID: (Human) O95352, (Rat) Q641Y5, (Mouse) Q9D906

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Entrez Gene ID: (Human) 10533, (Pig) 100462749, (Rat) 312647, (Mouse) 74244

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Function(s)

ubiquitin activating enzyme activity protein binding transcription factor binding Atg12 activating enzyme activity Atg8 activating enzyme activity protein homodimerization activity molecular_function small protein activating enzyme activity ubiquitin-protein ligase

Process(es)

cellular protein modification process protein lipidation C-terminal protein lipidation cellular response to nitrogen starvation cellular response to starvation positive regulation of autophagy protein transport macroautophagy positive regulation of macroautophagy protein ubiquitination positive regulation of protein modification process piecemeal microautophagy of nucleus suppression by virus of host autophagy positive regulation of apoptotic process late nucleophagy positive regulation of protein catabolic process defense response to virus membrane fusion cellular response to hyperoxia regulation of mitophagy mitophagy liver development cellular amino acid metabolic process autophagy organelle organization mitochondrion organization adult walking behavior post-embryonic development cerebellar Purkinje cell layer development pyramidal neuron development central nervous system neuron axonogenesis cerebral cortex development protein catabolic process neuron projection development regulation of protein ubiquitination protein modification by small protein conjugation negative stranded viral RNA replication response to starvation negative regulation of apoptotic process neurological system process cardiac muscle cell development membrane organization positive regulation of mucus secretion negative regulation of histone H4-K16 acetylation autophagosome assembly transport regulation of cell development negative regulation of cell death regulation of hemopoiesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG7 Monoclonal Antibody (2B5A10)

ATG7 Antibody (67341-1-IG) in ICC/IF

Product Details

67341-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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