Invitrogen

ATGL Monoclonal Antibody (K.751.7)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

9 Published Figures

6 References

View all (18) ATGL antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ATGL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

ATGL Antibody (MA5-14990) in ICC/IF

Immunofluorescence analysis of ATGL was performed using 70% confluent log phase 3T3-L1 and 3T3-L1 differentiated to adipocytes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Anti... View More

ATGL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATGL Antibody in Western Blot, Immunoprecipitation (WB, IP)

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ATGL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using ATGL Monoclonal Antibody (K.751.7) (Product # MA5-14990) shows increased expression of ATGL in 3T3-L1 differentiated adipocytes in comparison to 3T3-L1. {RE}

Product Details

MA5-14990

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.751.7

Immunogen

Synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

23 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10974333

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

MA5-14990 was used in Western Blotting to investigate the antiobesity and antidiabetic effects of the dairy bacterium P. freudenreichii MJ2 isolated from raw milk using 3T3-L1 cells and high-fat diet (HFD)-induced obese mice.

Thu Jan 28 00:00:00 EST 2021

Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.

Scientific reports

An M,Park YH,Lim YH

Thu Jan 28 00:00:00 EST 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

MA5-14990 was used in Western Blotting to investigate the mechanisms of Ca2+ oscillation generation upon activation of connexin-43 and regulation of the lipolysis/lipogenesis balance in white adipocytes through vesicular ATP release.

Wed Jul 28 00:00:00 EDT 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

Wed Jul 28 00:00:00 EDT 2021

Myocardial Adipose Triglyceride Lipase Overexpression Protects against Burn-Induced Cardiac Lipid Accumulation and Injury.

Oxidative medicine and cellular longevity

Li L,Zhang X,Zhang Q,Jia J,Zhang J,Zhang D,Song H,Chen B,Hu J,Huang Y

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Fri Dec 13 00:00:00 EST 2019

Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage.

The FEBS journal

Chen W,Jiang Y,Han J,Hu J,He T,Yan T,Huang N,Zhang Q,Mei H,Liao Y,Huang Y,Chen B

Published figure using ATGL monoclonal antibody (Product # MA5-14990) in Western Blot

Sat Apr 01 00:00:00 EDT 2017

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

MA5-14990 was used in immunoprecipitation to study the interaction of FSP27 with adipose triglyceride lipase and its role in regulating adipocyte lipolysis and insulin signaling

Fri Apr 25 00:00:00 EDT 2014

Fat-specific protein 27 (FSP27) interacts with adipose triglyceride lipase (ATGL) to regulate lipolysis and insulin sensitivity in human adipocytes.

The Journal of biological chemistry

Grahn THM,Kaur R,Yin J,Schweiger M,Sharma VM,Lee MJ,Ido Y,Smas CM,Zechner R,Lass A,Puri V

MA5-14990 was used in immunoprecipitation to study the interaction of FSP27 with adipose triglyceride lipase and its role in regulating adipocyte lipolysis and insulin signaling

Fri Apr 25 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Adipose triglyceride lipase; ATGL DESNUTRIN; Calcium-independent phospholipase A2-zeta; Desnutrin; DKFZp667M109; IPLA2 zeta; iPLA2-zeta; Patatin-like phospholipase domain-containing protein 2; PEDF R; plpl; plpl2; transport-secretion protein; triglyceride hydrolase; TTS 2.2

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Gene Aliases: 0610039C21Rik; 1110001C14Rik; Atgl; Pnpla2; RGD1309044; TTS-2.2

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UniProt ID: (Rat) P0C548, (Mouse) Q8BJ56

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Entrez Gene ID: (Rat) 361676, (Mouse) 66853

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Function(s)

triglyceride lipase activity protein binding hydrolase activity phospholipase

Process(es)

negative regulation of sequestering of triglyceride positive regulation of triglyceride catabolic process triglyceride catabolic process lipid metabolic process metabolic process lipid catabolic process lipid storage lipid particle organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATGL Monoclonal Antibody (K.751.7)

ATGL Antibody (MA5-14990) in ICC/IF

Product Details

MA5-14990

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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