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ATM Polyclonal Antibody

ATM Antibody in Immunocytochemistry (ICC/IF)

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ATM Antibody (27156-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 27156-1-AP) (ATM antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ATM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

27156-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 24 publications 24 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATM Fusion Protein Ag25909 (2407-2709 aa encoded by BC137169)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SSEFENKQA LLKRAKEEVG LLREHKIQTN RYTVKVQREL ELDELALRAL KEDRKRFLCK AVENYINCLL SGEEHDMWVF RLCSLWLENS GVSEVNGMMK RDGMKIPTYK FLPLMYQLAA RMGTKMMGGL GFHEVLNNLI SRISMDHPHH TLFIILALAN ANRDEFLTKP EVARRSRITK NVPKQSSQLD EDRTEAANRI ICTIRSRRPQ MVRSVEALCD AYIILANLDA TQWKTQRKGI NIPADQPITK LKNLEDVVVP TMEIKVDHTG EYGNLVTIQS FKAEFRLAGG VNLPKIIDCV GSDG (2407-2709 aa encoded by BC137169)

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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PGC1α Degradation Suppresses Mitochondrial Biogenesis to Confer Radiation Resistance in Glioma.

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Zhao M,Li Y,Lu C,Ding F,Xu M,Ge X,Li M,Wang Z,Yin J,Zhang J,Wang X,Ge Z,Xiao H,Xiao Y,Liu H,Liu W,Cao Y,Wang Q,You Y,Wang X,Yang K,Shi Z,Qian X

Tue Apr 04 00:00:00 EDT 2023

Nerve Injury-Induced γH2AX Reduction in Primary Sensory Neurons Is Involved in Neuropathic Pain Processing.

International journal of molecular sciences

Zhang Y,Gong H,Wang JS,Li MN,Cao DL,Gu J,Zhao LX,Zhang XD,Deng YT,Dong FL,Gao YJ,Sun WX,Jiang BC

Thu Jun 15 00:00:00 EDT 2023

TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex.

Oncogene

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Single-cell profiling-guided combination therapy of c-Fos and histone deacetylase inhibitors in diffuse large B-cell lymphoma.

Clinical and translational medicine

Wang L,Wu Z,Xia Y,Lu X,Li J,Fan L,Qiao C,Qiu H,Gu D,Xu W,Li J,Jin H

Sun May 01 00:00:00 EDT 2022

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway.

Frontiers in oncology

Wen X,Chen S,Chen X,Qiu H,Wang W,Zhang N,Liu W,Wang T,Ding X,Zhang L

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Targeting MDC1 promotes apoptosis and sensitizes Imatinib resistance in CML cells by mainly disrupting non-homologous end-joining repair.

Medical oncology (Northwood, London, England)

Liang Y,Qin Y,Jiang G,Feng W,Yuan Y

Thu Sep 29 00:00:00 EDT 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

Acquired temozolomide resistance in MGMT^low gliomas is associated with regulation of homologous recombination repair by ROCK2.

Cell death & disease

Zhang X,Li T,Yang M,Du Q,Wang R,Fu B,Tan Y,Cao M,Chen Y,Wang Q,Hu R

Thu Feb 10 00:00:00 EST 2022

p53-dependent elimination of aneuploid mitotic offspring by entosis.

Cell death and differentiation

Liang J,Niu Z,Zhang B,Yu X,Zheng Y,Wang C,Ren H,Wang M,Ruan B,Qin H,Zhang X,Gu S,Sai X,Tai Y,Gao L,Ma L,Chen Z,Huang H,Wang X,Sun Q

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AIMP3 inhibits cell growth and metastasis of lung adenocarcinoma through activating a miR-96-5p-AIMP3-p53 axis.

Journal of cellular and molecular medicine

Ding L,Fang Y,Li Y,Hu Q,Ai M,Deng K,Huang X,Xin H

Mon Mar 01 00:00:00 EST 2021

Upregulation of Chemoresistance by Mg²⁺ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.

Cells

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MAP4K1 functions as a tumor promotor and drug mediator for AML via modulation of DNA damage/repair system and MAPK pathway.

EBioMedicine

Ling Q,Li F,Zhang X,Mao S,Lin X,Pan J,Ye W,Wei W,Qian Y,Hu C,Huang X,Wang J,Wang H,Huang J,Wang Y,Jin J

Thu Jul 01 00:00:00 EDT 2021

MET inhibition enhances PARP inhibitor efficacy in castration-resistant prostate cancer by suppressing the ATM/ATR and PI3K/AKT pathways.

Journal of cellular and molecular medicine

Zhou S,Dai Z,Wang L,Gao X,Yang L,Wang Z,Wang Q,Liu Z

Wed Dec 01 00:00:00 EST 2021

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Journal of cellular and molecular medicine

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Wed Dec 01 00:00:00 EST 2021

Mutant IDH1 Enhances Temozolomide Sensitivity via Regulation of the ATM/CHK2 Pathway in Glioma.

Cancer research and treatment

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Thu Apr 01 00:00:00 EDT 2021

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American journal of cancer research

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Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

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Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

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Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

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2-Acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylcarbonylamino) phenyl carbamoylsulfanyl] propionic acid, a glutathione reductase inhibitor, induces G₂/M cell cycle arrest through generation of thiol oxidative stress in human esophageal cancer cells.

Oncotarget

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Oncotarget

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Bioinformatics

Protein Aliases: A-T mutated; AT mutated; AT mutated protein; Ataxia t; Ataxia telangiectasia mutated; DKFZp781A0353; EC 2.7.1.37; MGC74674; Serine-protein kinase ATM; TEL1, telomere maintenance 1, homolog

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Gene Aliases: AT1; ATA; ATC; ATD; ATDC; ATE; ATM; TEL1; TELO1

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UniProt ID: (Human) Q13315

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Entrez Gene ID: (Human) 472

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Function(s)

DNA binding protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity protein complex binding protein dimerization activity protein N-terminus binding non-receptor serine/threonine protein kinase

Process(es)

double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement response to hypoxia somitogenesis pre-B cell allelic exclusion DNA replication DNA repair double-strand break repair via nonhomologous end joining protein phosphorylation cellular response to DNA damage stimulus DNA damage induced protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest telomere maintenance via telomerase cell cycle arrest mitotic spindle assembly checkpoint reciprocal meiotic recombination signal transduction brain development heart development determination of adult lifespan intrinsic apoptotic signaling pathway in response to DNA damage response to ionizing radiation regulation of autophagy histone phosphorylation peptidyl-serine phosphorylation negative regulation of B cell proliferation positive regulation of telomere maintenance via telomerase positive regulation of histone phosphorylation V(D)J recombination phosphatidylinositol-3-phosphate biosynthetic process peptidyl-serine autophosphorylation lipoprotein catabolic process regulation of apoptotic process positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of neuron apoptotic process meiotic telomere clustering protein autophosphorylation oocyte development neuron apoptotic process regulation of telomerase activity histone mRNA catabolic process cellular response to gamma radiation cellular response to X-ray cellular response to nitrosative stress signal transduction involved in mitotic G2 DNA damage checkpoint replicative senescence establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere regulation of cellular response to heat regulation of signal transduction by p53 class mediator negative regulation of TORC1 signaling negative regulation of telomere capping positive regulation of telomere maintenance via telomere lengthening positive regulation of telomerase catalytic core complex assembly

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ATM Polyclonal Antibody

ATM Antibody (27156-1-AP) in ICC/IF

Product Details

27156-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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