Invitrogen

ATM Monoclonal Antibody (2C1)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures

5 References

View all (74) ATM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ATM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

ATM Antibody (MA1-23152) in IHC (P)

Immunohistochemistry (Paraffin) analysis. Human Testis (formalin-fixed, paraffin-embedded) stained with ATM Monoclonal Antibody (2C1) (Product # MA1-23152) at 5 µg/mL followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Product Image Gallery

ATM Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inATM KO cell line compared to control cell line using Anti-ATM Monoclonal Antibody (2C1) (Product # MA1-23152). {KO}

Product Details

MA1-23152

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

1-10 µg/mL

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2C1

Immunogen

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.99 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557657

Product Specific Information

Recommended positive controls: SK-N-SH, HeLa, HeLa nuclear extract.

Predicted molecular weight of MA1-23152 is ~350kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Upregulated DNA Damage-Linked Biomarkers in Parkinson's Disease Model Mice.

ASN neuro

Zeng F,Parker K,Zhan Y,Miller M,Zhu MY

Published figure using ATM monoclonal antibody (Product # MA1-23152) in Western Blot

Tue Feb 28 00:00:00 EST 2023

SMPDL3b modulates radiation-induced DNA damage response in renal podocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Francis M,Ahmad A,Bodgi L,Azzam P,Youssef T,Abou Daher A,Eid AA,Fornoni A,Pollack A,Marples B,Zeidan YH

MA1-23152 was used in ChIP Assay, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to unravel a novel role for SMPDL3b in radiation-induced DNA damage response - modulating nuclear sphingolipid metabolism, ATM nuclear shuttling, and DSBs repair.

Sat Oct 01 00:00:00 EDT 2022

DNA-PK Inhibitor, M3814, as a New Combination Partner of Mylotarg in the Treatment of Acute Myeloid Leukemia.

Frontiers in oncology

Carr MI,Zimmermann A,Chiu LY,Zenke FT,Blaukat A,Vassilev LT

Published figure using ATM monoclonal antibody (Product # MA1-23152) in Western Blot

Mon Sep 28 00:00:00 EDT 2020

Repair genes expression profile of MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers.

Archives of oral biology

Alves MG,Carta CF,de Barros PP,Issa JS,Nunes FD,Almeida JD

MA123152 was used in immunocytochemistry to determine the effect of chronic smoking on repair gene expression in the normal oral mucosa of chronic smokers and never smokers

Sun Jan 01 00:00:00 EST 2017

A novel porcine model of ataxia telangiectasia reproduces neurological features and motor deficits of human disease.

Human molecular genetics

Beraldi R,Chan CH,Rogers CS,Kovács AD,Meyerholz DK,Trantzas C,Lambertz AM,Darbro BW,Weber KL,White KA,Rheeden RV,Kruer MC,Dacken BA,Wang XJ,Davis BT,Rohret JA,Struzynski JT,Rohret FA,Weimer JM,Pearce DA

Published figure using ATM monoclonal antibody (Product # MA1-23152) in Western Blot

Sun Nov 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: A-T mutated; A-T mutated homolog; AT mutated; AT mutated protein; Ataxia t; Ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; DKFZp781A0353; EC 2.7.1.37; MGC74674; Serine-protein kinase ATM; TEL1, telomere maintenance 1, homolog

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Gene Aliases: AI256621; AT1; ATA; ATC; ATD; ATDC; ATE; ATM; C030026E19Rik; TEL1; TELO1

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UniProt ID: (Human) Q13315, (Mouse) E9QNY7

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Entrez Gene ID: (Human) 472, (Mouse) 11920, (Rat) 300711

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Function(s)

DNA binding protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity protein complex binding protein dimerization activity protein N-terminus binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor histone serine kinase activity non-receptor serine/threonine protein kinase

Process(es)

double-strand break repair via homologous recombination DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement response to hypoxia somitogenesis pre-B cell allelic exclusion DNA replication DNA repair double-strand break repair via nonhomologous end joining protein phosphorylation cellular response to DNA damage stimulus DNA damage induced protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest telomere maintenance via telomerase cell cycle arrest mitotic spindle assembly checkpoint reciprocal meiotic recombination signal transduction brain development heart development determination of adult lifespan intrinsic apoptotic signaling pathway in response to DNA damage response to ionizing radiation regulation of autophagy histone phosphorylation peptidyl-serine phosphorylation negative regulation of B cell proliferation positive regulation of telomere maintenance via telomerase positive regulation of histone phosphorylation V(D)J recombination phosphatidylinositol-3-phosphate biosynthetic process peptidyl-serine autophosphorylation lipoprotein catabolic process regulation of apoptotic process positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of neuron apoptotic process meiotic telomere clustering protein autophosphorylation oocyte development neuron apoptotic process regulation of telomerase activity histone mRNA catabolic process cellular response to gamma radiation cellular response to X-ray cellular response to nitrosative stress signal transduction involved in mitotic G2 DNA damage checkpoint replicative senescence establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere regulation of cellular response to heat regulation of signal transduction by p53 class mediator negative regulation of TORC1 signaling negative regulation of telomere capping positive regulation of telomere maintenance via telomere lengthening positive regulation of telomerase catalytic core complex assembly DNA damage checkpoint telomere maintenance cell cycle female gamete generation phosphorylation histone-serine phosphorylation phosphatidylinositol phosphorylation regulation of cell cycle apoptotic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATM Monoclonal Antibody (2C1)

ATM Antibody (MA1-23152) in IHC (P)

Product Details

MA1-23152

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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