Invitrogen

ATP Synthase beta Monoclonal Antibody (4.3E8.D10)

ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)

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ATP Synthase beta Antibody (MA1-930) in ICC/IF

Immunofluorescent analysis of ATP Synthase beta (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ATP Synthase beta mouse monoclonal antibody (Product # MA1-930), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with ... View More

ATP Synthase beta Antibody in Western Blot (WB)

ATP Synthase beta Antibody in Immunoprecipitation (IP)

ATP Synthase beta Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-930

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 6 publications 6 publications

Immunoprecipitation (IP)

2-5 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4.3E8.D10

Immunogen

Intact rat mitochondria.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2227740

Product Specific Information

MA1-930 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.

MA1-930 has been successfully used in immunofluorescence, western blot, and immunoprecipitation procedures. By immunoprecipitation, this antibody detects an 50 kDa protein representing ATP synthase from solubilized rat brain mitochondria.

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhancing Proteotoxic Stress in Leiomyosarcoma Cells Triggers Mitochondrial Dysfunctions, Cell Death, and Antitumor Activity in vivo.

Molecular cancer therapeutics

Iuliano L,Drioli S,Pignochino Y,Cafiero CM,Minisini M,D'Este F,Picco R,Dalla E,Giordano G,Grignani G,Di Giorgio E,Benedetti F,Felluga F,Brancolini C

MA1-930 was used in Immunocytochemistry-immunoflourescence to evaluate the induction of proteotoxic stress as a possible strategy to kill leiomyosarcoma cells in a therapeutic perspective.

Tue Jun 01 00:00:00 EDT 2021

Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica.

Journal of immunology (Baltimore, Md. : 1950)

Marrocco A,Frawley K,Pearce LL,Peterson J,O'Brien JP,Mullett SJ,Wendell SG,St Croix CM,Mischler SE,Ortiz LA

MA1-930 was used in Immunocytochemistry to highlight the importance of complex II activity and tricarboxylic acid cycle remodeling to macrophage survival and cytokine-mediated inflammation in silicosis.

Wed Sep 15 00:00:00 EDT 2021

CARD19, the protein formerly known as BinCARD, is a mitochondrial protein that does not regulate Bcl10-dependent NF-κB activation after TCR engagement.

Cellular immunology

Rios KE,Kashyap AK,Maynard SK,Washington M,Paul S,Schaefer BC

MA1-930 was used in Immunocytochemistry to indicate that the only detectable translated product of C9orf89 is the mitochondrial protein CARD19, which does not play a discernible role in TCR-dependent, Bcl10-mediated signal transduction to Malt1 or NF-κB.

Thu Oct 01 00:00:00 EDT 2020

ACE overexpression in myeloid cells increases oxidative metabolism and cellular ATP.

The Journal of biological chemistry

Cao DY,Spivia WR,Veiras LC,Khan Z,Peng Z,Jones AE,Bernstein EA,Saito S,Okwan-Duodu D,Parker SJ,Giani JF,Divakaruni AS,Van Eyk JE,Bernstein KE

MA1-930 was used in Western Blotting to find that ACE overexpression by both macrophages and neutrophils is associated with a marked change in the metabolism of cells and that this appears to underpin some of the phenotypic differences between these cells and myeloid cells expressing WT levels of ACE.

Fri Jan 31 00:00:00 EST 2020

Age-dependent changes in intervertebral disc cell mitochondria and bioenergetics.

European cells & materials

Hartman R,Patil P,Tisherman R,St Croix C,Niedernhofer LJ,Robbins PD,Ambrosio F,Van Houten B,Sowa G,Vo N

MA1-930 was used in Immunocytochemistry-immunoflourescence to reveal that ageing affected mitochondrial number and function, particularly in NP cells.

Thu Oct 18 00:00:00 EDT 2018

UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase.

The Journal of biological chemistry

Braganza A,Li J,Zeng X,Yates NA,Dey NB,Andrews J,Clark J,Zamani L,Wang XH,St Croix C,O'Sullivan R,Garcia-Exposito L,Brodsky JL,Sobol RW

MA1-930 was used in Immunocytochemistry-Immunofluorescence to demonstrate that UBE3B is an E3 ubiquitin ligase and reveal that the enzyme is regulated by calmodulin.

Fri Feb 10 00:00:00 EST 2017

UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase.

The Journal of biological chemistry

Braganza A,Li J,Zeng X,Yates NA,Dey NB,Andrews J,Clark J,Zamani L,Wang XH,St Croix C,O'Sullivan R,Garcia-Exposito L,Brodsky JL,Sobol RW

MA1-930 was used in Immunocytochemistry-Immunofluorescence to demonstrate that UBE3B is an E3 ubiquitin ligase and reveal that the enzyme is regulated by calmodulin.

Fri Feb 10 00:00:00 EST 2017

PINK1 deficiency impairs mitochondrial homeostasis and promotes lung fibrosis.

The Journal of clinical investigation

Bueno M,Lai YC,Romero Y,Brands J,St Croix CM,Kamga C,Corey C,Herazo-Maya JD,Sembrat J,Lee JS,Duncan SR,Rojas M,Shiva S,Chu CT,Mora AL

MA1-930 was used in immunohistochemistry to study the contribution of mitochondrial function to idiopathic pulmonary fibrosis pathogenesis.

Sun Feb 01 00:00:00 EST 2015

Human brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH.

FEMS microbiology letters

Shin S,Kim KS

MA1-930 was used in western blot to investigate the molecular mechanism for the binding of E.coli to human brain microvascular endothelial cells

Mon Feb 01 00:00:00 EST 2010

The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.

The American journal of pathology

Addabbo F,Ratliff B,Park HC,Kuo MC,Ungvari Z,Csiszar A,Krasnikov B,Sodhi K,Zhang F,Nasjletti A,Goligorsky MS

MA1-930 was used in western blot to study the progression of endothelial cell dysfunction.

Thu Jan 01 00:00:00 EST 2009

Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.

American journal of physiology. Gastrointestinal and liver physiology

Metukuri MR,Beer-Stolz D,Namas RA,Dhupar R,Torres A,Loughran PA,Jefferson BS,Tsung A,Billiar TR,Vodovotz Y,Zamora R

MA1-930 was used in immunocytochemistry to study the association between the upregulation of BNIP3 and the hepatocyte cell death

Sun Mar 01 00:00:00 EST 2009

Polyamine transport by mammalian cells and mitochondria: role of antizyme and glycosaminoglycans.

The Journal of biological chemistry

Hoshino K,Momiyama E,Yoshida K,Nishimura K,Sakai S,Toida T,Kashiwagi K,Igarashi K

MA1-930 was used in western blot to investigate the roles of antizyme and glycosaminoglycans during the polyamine transport in mammalian cells and mitochondria.

Fri Dec 30 00:00:00 EST 2005

Bioinformatics

Protein Aliases: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

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UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

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Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947

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Function(s)

transporter activity protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism calcium ion binding lipoprotein particle receptor activity nucleotide binding hydrolase activity hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ATP synthase

Process(es)

angiogenesis osteoblast differentiation generation of precursor metabolites and energy lipid metabolic process ATP biosynthetic process negative regulation of cell adhesion involved in substrate-bound cell migration mitochondrion organization ATP hydrolysis coupled proton transport proton transport mitochondrial ATP synthesis coupled proton transport regulation of intracellular pH receptor-mediated endocytosis ATP synthesis coupled proton transport ATP metabolic process transport ion transport hydrogen ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP Synthase beta Monoclonal Antibody (4.3E8.D10)

ATP Synthase beta Antibody (MA1-930) in ICC/IF

Product Details

MA1-930

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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