Invitrogen

ATP5A1 Monoclonal Antibody (15H4C4)

7 Published Figures

19 References

View all (32) ATP5A1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (7)

ATP5A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

ATP5A1 Antibody (43-9800) in ICC/IF

Immunofluorescence analysis of ATP5A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP5A1 Monoclonal Antibody (15H4C4) (Product # 43-9800) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a... View More

ATP5A1 Antibody in Flow Cytometry (Flow)

ATP5A1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

43-9800

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 13 publications 13 publications

Immunohistochemistry (IHC)

1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Fruit fly, Human, Mouse, Rat

Published species

C. elegans, Fruit fly, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

15H4C4

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2533548

Product Specific Information

When performing IHC use heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Positive control: Human, bovine, mouse and rat heart mitochondria.

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

M1BP is an essential transcriptional activator of oxidative metabolism during Drosophila development.

Nature communications

Poliacikova G,Barthez M,Rival T,Aouane A,Luis NM,Richard F,Daian F,Brouilly N,Schnorrer F,Maurel-Zaffran C,Graba Y,Saurin AJ

Published figure using ATP5A1 monoclonal antibody (Product # 43-9800) in Western Blot

Fri Jun 02 00:00:00 EDT 2023

Inhibition of ATP synthase reverse activity restores energy homeostasis in mitochondrial pathologies.

The EMBO journal

Acin-Perez R,Benincá C,Fernandez Del Rio L,Shu C,Baghdasarian S,Zanette V,Gerle C,Jiko C,Khairallah R,Khan S,Rincon Fernandez Pacheco D,Shabane B,Erion K,Masand R,Dugar S,Ghenoiu C,Schreiner G,Stiles L,Liesa M,Shirihai OS

43-9800 was used in Immunocytochemistry-immunofluorescence to conclude that the impact of compromised mitochondrial respiration can be lessened using hydrolysis-selective inhibitors of CV.

Mon May 15 00:00:00 EDT 2023

Inhibition of ATP synthase reverse activity restores energy homeostasis in mitochondrial pathologies.

The EMBO journal

43-9800 was used in Immunocytochemistry-immunofluorescence to conclude that the impact of compromised mitochondrial respiration can be lessened using hydrolysis-selective inhibitors of CV.

Mon May 15 00:00:00 EDT 2023

Combining albumin deficiency and acute exercise reduces hepatic lipid droplet size in mice.

Lipids in health and disease

Zhang Y,Szramowski M,Sun S,Henderson GC

43-9800 was used in Western Blotting to investigate the combined effect of albumin deficiency and acute exercise on hepatic lipid droplets (LDs).

Wed Jun 21 00:00:00 EDT 2023

mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.

Frontiers in cell and developmental biology

Liu P,Chang K,Requejo G,Bai H

43-9800 was used in Immunohistochemistry-immunofluorescence to find that knockdown of mTORC2 subunit rictor blocks HFD-induced mitochondrial fragmentation and Drp1 recruitment.

Thu Sep 29 00:00:00 EDT 2022

mTORC2 protects the heart from high-fat diet-induced cardiomyopathy through mitochondrial fission in Drosophila.

Frontiers in cell and developmental biology

Liu P,Chang K,Requejo G,Bai H

43-9800 was used in Immunohistochemistry-immunofluorescence to find that knockdown of mTORC2 subunit rictor blocks HFD-induced mitochondrial fragmentation and Drp1 recruitment.

Thu Sep 29 00:00:00 EDT 2022

mt tRFs, New Players in MELAS Disease.

Frontiers in physiology

Meseguer S,Rubio MP

43-9800 was used in Immunocytochemistry-immunofluorescence to suggest an involvement of Dicer and Ago2 in the mechanism of mt tRF biogenesis and action.

Mon Mar 14 00:00:00 EDT 2022

mt tRFs, New Players in MELAS Disease.

Frontiers in physiology

Meseguer S,Rubio MP

43-9800 was used in Immunocytochemistry-immunofluorescence to suggest an involvement of Dicer and Ago2 in the mechanism of mt tRF biogenesis and action.

Mon Mar 14 00:00:00 EDT 2022

Bacterial peptidoglycan muropeptides benefit mitochondrial homeostasis and animal physiology by acting as ATP synthase agonists.

Developmental cell

Tian D,Han M

43-9800 was used in Western Blot to identify a role for muropeptides in supporting mitochondrial homeostasis, development, and behaviors in Caenorhabditis elegans.

Mon Feb 07 00:00:00 EST 2022

Generation and Characterization of Germline-Specific Autophagy and Mitochondrial Reactive Oxygen Species Reporters in Drosophila.

Frontiers in cell and developmental biology

Nilangekar K,Murmu N,Sahu G,Shravage BV

43-9800 was used in Immunohistochemistry to provide a valuable tool that can be utilized in designing genetic screens to identify novel regulators of autophagy and redox homeostasis during oogenesis.

Tue Sep 29 00:00:00 EDT 2020

Condensin II protein dysfunction impacts mitochondrial respiration and mitochondrial oxidative stress responses.

Journal of cell science

Deutschman E,Ward JR,Kumar A,Ray G,Welch N,Lemieux ME,Dasarathy S,Longworth MS

43-9800 was used in Western Blot to identify condensin II as novel regulators of mitochondrial respiration and mitochondrial stress responses.

Wed Nov 20 00:00:00 EST 2019

Mitochondrial Oxidative Phosphorylation defect in the Heart of Subjects with Coronary Artery Disease.

Scientific reports

Ait-Aissa K,Blaszak SC,Beutner G,Tsaih SW,Morgan G,Santos JH,Flister MJ,Joyce DL,Camara AKS,Gutterman DD,Donato AJ,Porter GA,Beyer AM

43-9800 was used in Western Blotting to study via PCR analysis to reveal an increase in mtDNA lesions and the frequency of mitochondrial common deletion, both established markers for impaired mitochondrial integrity in CAD compared to non-CAD patient samples.

Mon May 20 00:00:00 EDT 2019

Fascin Controls Metastatic Colonization and Mitochondrial Oxidative Phosphorylation by Remodeling Mitochondrial Actin Filaments.

Cell reports

Lin S,Huang C,Gunda V,Sun J,Chellappan SP,Li Z,Izumi V,Fang B,Koomen J,Singh PK,Hao J,Yang S

43-9800 was used in Western Blot to report that fascin, an actin-bundling protein, promotes lung cancer metastatic colonization by augmenting metabolic stress resistance and mitochondrial oxidative phosphorylation (OXPHOS).

Tue Sep 10 00:00:00 EDT 2019

SNX14 mutations affect endoplasmic reticulum-associated neutral lipid metabolism in autosomal recessive spinocerebellar ataxia 20.

Human molecular genetics

Bryant D,Liu Y,Datta S,Hariri H,Seda M,Anderson G,Peskett E,Demetriou C,Sousa S,Jenkins D,Clayton P,Bitner-Glindzicz M,Moore GE,Henne WM,Stanier P

43-9800 was used in Western Blotting to determine the role of SNX14 in endoplasmic reticulum-lipid droplet crosstalk.

Fri Jun 01 00:00:00 EDT 2018

MICU1 Confers Protection from MCU-Dependent Manganese Toxicity.

Cell reports

Wettmarshausen J,Goh V,Huang KT,Arduino DM,Tripathi U,Leimpek A,Cheng Y,Pittis AA,Gabaldón T,Mokranjac D,Hajnóczky G,Perocchi F

43-9800 was used in Western Blot to dissect the interdependence between the pore-forming subunit MCU and the calcium-sensing regulator MICU1.

Tue Nov 06 00:00:00 EST 2018

Regulation of autophagy, mitochondrial dynamics, and cellular bioenergetics by 4-hydroxynonenal in primary neurons.

Autophagy

Dodson M,Wani WY,Redmann M,Benavides GA,Johnson MS,Ouyang X,Cofield SS,Mitra K,Darley-Usmar V,Zhang J

43-9800 was used in Western Blotting to show that mitochondria and autophagy are critical targets of 4-hydroxynonenal, and that the proteins targeted by 4-hydroxynonenal may change based on its concentration, leading to cellular dysfunction.

Tue Jun 04 00:00:00 EDT 2019

Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.

Journal of cell science

Vagnoni A,Hoffmann PC,Bullock SL

43-9800 was used in western blot to learn about augmentation of mitochondrial transport and protective effect in adult Drosophila neurons by reducing lissencephaly-1 levels

Fri Jan 01 00:00:00 EST 2016

Downstream effects of plectin mutations in epidermolysis bullosa simplex with muscular dystrophy.

Acta neuropathologica communications

Winter L,Türk M,Harter PN,Mittelbronn M,Kornblum C,Norwood F,Jungbluth H,Thiel CT,Schlötzer-Schrehardt U,Schröder R

43-9800 was used in western blot to study muscular dystrophy and the downstream effects of plectin mutations in epidermolysis bullosa simplex

Wed Apr 27 00:00:00 EDT 2016

Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.

Free radical biology & medicine

Dun Y,Vargas J,Brot N,Finnemann SC

43-9800 was used in western blot to test if and how MsrA affects retinal pigment epithelium functionality.

Sun Dec 01 00:00:00 EST 2013

A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.

BioTechniques

Song S,Kole S,Bernier M

Sun Apr 01 00:00:00 EDT 2012

A chemical cross-linking method for the analysis of binding partners of heat shock protein-90 in intact cells.

BioTechniques

Song S,Kole S,Bernier M

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: alpha subunit ATP synthase isoform precursor (EC 3.6.1.34); ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase alpha subunit precursor; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

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UniProt ID: (Human) P25705, (Bovine) P19483, (Rat) P15999, (Mouse) Q03265

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Entrez Gene ID: (Human) 498, (Bovine) 282578, (Rat) 65262, (Mouse) 11946

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Function(s)

protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ATP synthase

Process(es)

negative regulation of endothelial cell proliferation lipid metabolic process ATP biosynthetic process embryo development ATP hydrolysis coupled proton transport mitochondrial ATP synthesis coupled proton transport ATP synthesis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport transport ion transport proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP5A1 Monoclonal Antibody (15H4C4)

ATP5A1 Antibody (43-9800) in ICC/IF

Product Details

43-9800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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