Proteintech

ATP5A1 Monoclonal Antibody (1B10H3)

ATP5A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

ATP5A1 Antibody (66037-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATP5A1 antibody (Product # 66037-1-Ig) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin.

ATP5A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP5A1 Antibody in Flow Cytometry (Flow)

ATP5A1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66037-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1B10H3

Immunogen

ATP5A1 Fusion Protein Ag8119 (202-553 aa encoded by BC064562)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Immunogen sequence: IGRGQRELI IGDRQTGKTS IAIDTIINQK RFNDGSDEKK KLYCIYVAIG QKRSTVAQLV KRLTDADAMK YTIVVSATAS DAAPLQYLAP YSGCSMGEYF RDNGKHALII YDDLSKQAVA YRQMSLLLRR PPGREAYPGD VFYLHSRLLE RAAKMNDAFG GGSLTALPVI ETQAGDVSAY IPTNVISITD GQIFLETELF YKGIRPAINV GLSVSRVGSA AQTRAMKQVA GTMKLELAQY REVAAFAQFG SDLDAATQQL LSRGVRLTEL LKQGQYSPMA IEEQVAVIYA GVRGYLDKLE PSKITKFENA FLSHVVSQHQ ALLGTIRADG KISEQSDAKL KEIVTNFLAG FEA (202-553 aa encoded by BC064562)

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects.

The Science of the total environment

Li D,Zhao Q,Xie L,Wang C,Tian Z,Tang H,Xia T,Wang A

Sat Apr 15 00:00:00 EDT 2023

Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Zheng M,Hu Z,Wang Y,Wang C,Zhong C,Cui W,You J,Gao B,Sun X,La L

Sun Jan 01 00:00:00 EST 2023

Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.

Phytotherapy research : PTR

Cheng L,Shi L,He C,Wang C,Lv Y,Li H,An Y,Dai H,Duan Y,Zhang H,Huang Y,Fu W,Meng Y,Zhao B

Wed Jun 01 00:00:00 EDT 2022

Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy.

Pharmacological research

Luo L,Luo J,Cai Y,Fu M,Li W,Shi L,Liu J,Dong R,Xu X,Tu L,Yang Y

Thu Sep 01 00:00:00 EDT 2022

Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu H,Liu Y,Wang H,Zhao Q,Zhang T,Xie SA,Liu Y,Tang Y,Peng Q,Pang W,Yao W,Zhou J

Tue Nov 01 00:00:00 EDT 2022

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N⁶-methyladenosine.

Developmental cell

Lv D,Gimple RC,Zhong C,Wu Q,Yang K,Prager BC,Godugu B,Qiu Z,Zhao L,Zhang G,Dixit D,Lee D,Shen JZ,Li X,Xie Q,Wang X,Agnihotri S,Rich JN

Mon Jun 20 00:00:00 EDT 2022

Berberine mitigates hepatic insulin resistance by enhancing mitochondrial architecture via the SIRT1/Opa1 signalling pathway.

Acta biochimica et biophysica Sinica

Xu J,Zhang Y,Yu Z,Guan Y,Lv Y,Zhang M,Zhang M,Chen L,Lv X,Guan F

Tue Oct 25 00:00:00 EDT 2022

CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis.

Diabetes

Ding M,Ma YJ,Du RQ,Zhou WY,Dou X,Yang QQ,Tang Y,Qian SW,Liu Y,Pan DN,Tang QQ,Liu Y

Thu Sep 01 00:00:00 EDT 2022

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Acta biochimica et biophysica Sinica

Chen F,Yan H,Guo C,Zhu H,Yi J,Sun X,Yang J

Tue Aug 31 00:00:00 EDT 2021

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Acta biochimica et biophysica Sinica

Chen F,Yan H,Guo C,Zhu H,Yi J,Sun X,Yang J

Tue Aug 31 00:00:00 EDT 2021

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Huang S,Wang FJ,Lin H,Liu T,Zhao CX,Chen LG

Mon Feb 01 00:00:00 EST 2021

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M,Friedman J,Jost M,Xu J,Yamada J,Kampmann M,Horlbeck MA,Salemi MR,Phinney BS,Weissman JS,Nunnari J

Wed May 26 00:00:00 EDT 2021

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Nature cell biology

Shi M,Huang XY,Ren XY,Wei XY,Ma Y,Lin ZZ,Liu DT,Song L,Zhao TJ,Li G,Yao L,Zhu M,Zhang C,Xie C,Wu Y,Wu HM,Fan LP,Ou J,Zhan YH,Lin SY,Lin SC

Mon Mar 01 00:00:00 EST 2021

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Cell death & disease

Wang P,Luo R,Zhang M,Wang Y,Song T,Tao T,Li Z,Jin L,Zheng H,Chen W,Zhao M,Zheng Y,Qin J

Tue Dec 08 00:00:00 EST 2020

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Journal of proteome research

Meng K,Lu S,Yan X,Sun Y,Gao J,Wang Y,Yin X,Sun Z,He QY

Fri Mar 06 00:00:00 EST 2020

Quantitative Mitochondrial Proteomics Reveals ANXA7 as a Crucial Factor in Mitophagy.

Journal of proteome research

Meng K,Lu S,Yan X,Sun Y,Gao J,Wang Y,Yin X,Sun Z,He QY

Fri Mar 06 00:00:00 EST 2020

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Autophagy

Ni Z,He J,Wu Y,Hu C,Dai X,Yan X,Li B,Li X,Xiong H,Li Y,Li S,Xu L,Li Y,Lian J,He F

Thu Mar 07 00:00:00 EST 2019

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Autophagy

Ni Z,He J,Wu Y,Hu C,Dai X,Yan X,Li B,Li X,Xiong H,Li Y,Li S,Xu L,Li Y,Lian J,He F

Thu Mar 07 00:00:00 EST 2019

Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

Scientific reports

Yajima Y,Hiratsuka T,Kakimoto Y,Ogawa S,Shima K,Yamazaki Y,Yoshikawa K,Tamaki K,Tsuruyama T

Thu May 10 00:00:00 EDT 2018

Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

Scientific reports

Yajima Y,Hiratsuka T,Kakimoto Y,Ogawa S,Shima K,Yamazaki Y,Yoshikawa K,Tamaki K,Tsuruyama T

Thu May 10 00:00:00 EDT 2018

Succinate induces aberrant mitochondrial fission in cardiomyocytes through GPR91 signaling.

Cell death & disease

Lu YT,Li LZ,Yang YL,Yin X,Liu Q,Zhang L,Liu K,Liu B,Li J,Qi LW

Mon Jun 04 00:00:00 EDT 2018

Succinate induces aberrant mitochondrial fission in cardiomyocytes through GPR91 signaling.

Cell death & disease

Lu YT,Li LZ,Yang YL,Yin X,Liu Q,Zhang L,Liu K,Liu B,Li J,Qi LW

Mon Jun 04 00:00:00 EDT 2018

Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol.

Biochimica et biophysica acta. Molecular basis of disease

Wu B,Luo H,Zhou X,Cheng CY,Lin L,Liu BL,Liu K,Li P,Yang H

Fri Sep 01 00:00:00 EDT 2017

Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol.

Biochimica et biophysica acta. Molecular basis of disease

Wu B,Luo H,Zhou X,Cheng CY,Lin L,Liu BL,Liu K,Li P,Yang H

Fri Sep 01 00:00:00 EDT 2017

Ginkgolide K attenuates neuronal injury after ischemic stroke by inhibiting mitochondrial fission and GSK-3β-dependent increases in mitochondrial membrane permeability.

Oncotarget

Zhou X,Wang HY,Wu B,Cheng CY,Xiao W,Wang ZZ,Yang YY,Li P,Yang H

Tue Jul 04 00:00:00 EDT 2017

Ginkgolide K attenuates neuronal injury after ischemic stroke by inhibiting mitochondrial fission and GSK-3β-dependent increases in mitochondrial membrane permeability.

Oncotarget

Zhou X,Wang HY,Wu B,Cheng CY,Xiao W,Wang ZZ,Yang YY,Li P,Yang H

Tue Jul 04 00:00:00 EDT 2017

Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice.

Proteomics

Du Y,Meng Y,Zhu J,Kang L,Jia X,Guo L,Zhang L,Ye M,Hu L,Zhao X,Gu J,Yang B,Zou H

Mon Sep 01 00:00:00 EDT 2014

Quantitative proteomic study of myocardial mitochondria in urea transporter B knockout mice.

Proteomics

Du Y,Meng Y,Zhu J,Kang L,Jia X,Guo L,Zhang L,Ye M,Hu L,Zhao X,Gu J,Yang B,Zou H

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: ATP synthase alpha chain, mitochondrial; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

View more View less

Gene Aliases: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM

View more View less

UniProt ID: (Human) P25705, (Rat) P15999, (Mouse) Q03265

View more View less

Entrez Gene ID: (Human) 498, (Rat) 65262, (Mouse) 11946

View more View less

Function(s)

protein binding ATP binding ATPase activity transmembrane transporter activity MHC class I protein binding poly(A) RNA binding proton-transporting ATP synthase activity, rotational mechanism proton-transporting ATPase activity, rotational mechanism ADP binding nucleotide binding hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ATP synthase

Process(es)

negative regulation of endothelial cell proliferation lipid metabolic process ATP biosynthetic process embryo development ATP hydrolysis coupled proton transport mitochondrial ATP synthesis coupled proton transport ATP synthesis coupled proton transport ATP metabolic process hydrogen ion transmembrane transport transport ion transport proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ATP5A1 Monoclonal Antibody (1B10H3)

ATP5A1 Antibody (66037-1-IG) in ICC/IF

Product Details

66037-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!