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ATP6V0D1 Polyclonal Antibody

View all (9) ATP6V0D1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (11)

ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF)

ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

ATP6V0D1 Antibody (18274-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF)

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP6V0D1 Antibody in Western Blot (WB)

ATP6V0D1 Antibody in Western Blot (WB)

ATP6V0D1 Antibody in Western Blot (WB)

ATP6V0D1 Antibody in Western Blot (WB)

ATP6V0D1 Antibody in Flow Cytometry (Flow)

ATP6V0D1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

ATP6V0D1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human kidney tissue slide using (Product # 18274-1-AP) ATP6V0D1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human kidney tissue slide using (Product # 18274-1-AP) ATP6V0D1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:100 (under 40x lens).

ATP6V0D1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:100 (under 10x lens).

ATP6V0D1 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ATP6V0D1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ATP6V0D1 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ATP6V0D1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 18274-1-AP (ATP6V0D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ATP6V0D1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug ATP6V0D1 antibody (18274-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

ATP6V0D1 Antibody in Immunoprecipitation (IP) — IP result of anti-ATP6V0D1 (IP:18274-1-AP, 4ug; Detection:18274-1-AP 1:500) with mouse testis tissue lysate 4800ug.

Product Details

18274-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATP6V0D1 Fusion Protein Ag13002 (1-351 aa encoded by BC008861)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSFFPELYF NVDNGYLEGL VRGLKAGVLS QADYLNLVQC ETLEDLKLHL QSTDYGNFLA NEASPLTVSV IDDRLKEKMV VEFRHMRNHA YEPLASFLDF ITYSYMIDNV ILLITGTLHQ RSIAELVPKC HPLGSFEQME AVNIAQTPAE LYNAILVDTP LAAFFQDCIS EQDLDEMNIE IIRNTLYKAY LESFYKFCTL LGGTTADAMC PILEFEADRR AFIITINSFG TELSKEDRAK LFPHCGRLYP EGLAQLARAD DYEQVKNVAD YYPEYKLLFE GAGSNPGDKT LEDRFFEHEV KLNKLAFLNQ FHFGVFYAFV KLKEQECRNI VWIAECIAQR HRAKIDNYIP IF (1-351 aa encoded by BC008861)

Target Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis.

Cell reports

Chen F,Zhu S,Kang R,Tang D,Liu J

Tue Jan 31 00:00:00 EST 2023

Identification of Novel Senescent Markers in Small Extracellular Vesicles.

International journal of molecular sciences

Misawa T,Hitomi K,Miyata K,Tanaka Y,Fujii R,Chiba M,Loo TM,Hanyu A,Kawasaki H,Kato H,Maezawa Y,Yokote K,Nakamura AJ,Ueda K,Yaegashi N,Takahashi A

Thu Jan 26 00:00:00 EST 2023

A Role for the V0 Sector of the V-ATPase in Neuroexocytosis: Exogenous V0d Blocks Complexin and SNARE Interactions with V0c.

Cells

Lévêque C,Maulet Y,Wang Q,Rame M,Rodriguez L,Mochida S,Sangiardi M,Youssouf F,Iborra C,Seagar M,Vitale N,El Far O

Sun Feb 26 00:00:00 EST 2023

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency.

Autophagy

Wang F,Yang Y,Boudagh G,Eskelinen EL,Klionsky DJ,Malek SN

Mon Aug 01 00:00:00 EDT 2022

Zinc enhances autophagic flux and lysosomal function through transcription factor EB activation and V-ATPase assembly.

Frontiers in cellular neuroscience

Kim KR,Park SE,Hong JY,Koh JY,Cho DH,Hwang JJ,Kim YH

Wed Oct 19 00:00:00 EDT 2022

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Cellular and molecular life sciences : CMLS

Castroflorio E,den Hoed J,Svistunova D,Finelli MJ,Cebrian-Serrano A,Corrochano S,Bassett AR,Davies B,Oliver PL

Thu Apr 01 00:00:00 EDT 2021

Upregulation of V-ATPase by STAT3 Activation Promotes Anoikis Resistance and Tumor Metastasis.

Journal of Cancer

Adeshakin FO,Adeshakin AO,Liu Z,Lu X,Cheng J,Zhang P,Yan D,Zhang G,Wan X

Fri Jul 09 00:00:00 EDT 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 EDT 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 EDT 2021

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhong B,Liu M,Bai C,Ruan Y,Wang Y,Qiu L,Hong Y,Wang X,Li L,Li B

Wed Apr 08 00:00:00 EDT 2020

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

Disruption of the vacuolar-type H⁺-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.

Autophagy

Kissing S,Rudnik S,Damme M,Lüllmann-Rauch R,Ichihara A,Kornak U,Eskelinen EL,Jabs S,Heeren J,De Brabander JK,Haas A,Saftig P

Mon Apr 03 00:00:00 EDT 2017

Disruption of the vacuolar-type H⁺-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes.

Autophagy

Kissing S,Rudnik S,Damme M,Lüllmann-Rauch R,Ichihara A,Kornak U,Eskelinen EL,Jabs S,Heeren J,De Brabander JK,Haas A,Saftig P

Mon Apr 03 00:00:00 EDT 2017

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.

The Journal of biological chemistry

Otomo T,Higaki K,Nanba E,Ozono K,Sakai N

Fri Oct 07 00:00:00 EDT 2011

Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.

The Journal of biological chemistry

Otomo T,Higaki K,Nanba E,Ozono K,Sakai N

Fri Oct 07 00:00:00 EDT 2011

Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.

The Journal of biological chemistry

Otomo T,Higaki K,Nanba E,Ozono K,Sakai N

Fri Oct 07 00:00:00 EDT 2011

Lysosomal storage causes cellular dysfunction in mucolipidosis II skin fibroblasts.

The Journal of biological chemistry

Otomo T,Higaki K,Nanba E,Ozono K,Sakai N

Fri Oct 07 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 32 kDa accessory protein; ATPase, H+ transporting, lysosomal (vacuolar proton pump), 42 kDa; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit D; ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1; ATPase, H+ transporting, V0 subunit D; FLJ43534; H(+)-transporting two-sector ATPase, subunit D; lysosomal 38kDa; P39; Physophilin; V-ATPase 40 KDa accessory protein; V-ATPase AC39 subunit; V-ATPase subunit d 1; V-ATPase, subunit D; V-type proton ATPa; V-type proton ATPase subunit d 1; Vacuolar proton pump subunit d 1

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Gene Aliases: Ac39; AI267038; ATP6D; ATP6DV; ATP6V0D1; P39; VATX; VMA6; VPATPD

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UniProt ID: (Human) P61421, (Mouse) P51863

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Entrez Gene ID: (Human) 9114, (Rat) 291969, (Mouse) 11972

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protein binding hydrogen-exporting ATPase activity, phosphorylative mechanism protein complex binding hydrogen ion transmembrane transporter activity ATP synthase

Process(es)

brain development insulin receptor signaling pathway ATP hydrolysis coupled proton transport proton transport regulation of macroautophagy transferrin transport ion transmembrane transport IRE1-mediated unfolded protein response cilium assembly phagosome acidification hydrogen ion transmembrane transport transport ion transport cell projection organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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