Proteintech

ATP6V1A Polyclonal Antibody

ATP6V1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

ATP6V1A Antibody (17115-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human pancreas using 17115-1-AP (ATP6V1A antibody) at dilution of 1:100 (under 40x lens).

ATP6V1A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17115-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATP6V1A Fusion Protein Ag10801 (268-617 aa encoded by BC013138)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NSDVIIYVG CGERGNEMSE VLRDFPELTM EVDGKVESIM KRTALVANTS NMPVAAREAS IYTGITLSEY FRDMGYHVSM MADSTSRWAE ALREISGRLA EMPADSGYPA YLGARLASFY ERAGRVKCLG NPEREGSVSI VGAVSPPGGD FSDPVTSATL GIVQVFWGLD KKLAQRKHFP SVNWLISYSK YMRALDEYYD KHFTEFVPLR TKAKEILQEE EDLAEIVQLV GKASLAETDK ITLEVAKLIK DDFLQQNGYT PYDRFCPFYK TVGMLSNMIA FYDMARRAVE TTAQSDNKIT WSIIREHMGD ILYKLSSMKF KDPLKDGEAK IKSDYAQLLE DMQNAFRSLE D (268-617 aa encoded by BC013138)

Target Information

ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells.

iScience

Ji X,Chu L,Su D,Sun J,Song P,Sun S,Wang Y,Mu Q,Liu Y,Wan Q

Fri May 19 00:00:00 EDT 2023

Molecular Physiological Evidence for the Role of Na⁺-Cl^- Co-Transporter in Branchial Na⁺ Uptake in Freshwater Teleosts.

International journal of molecular sciences

Shih SW,Yan JJ,Lu SW,Chuang YT,Lin HW,Chou MY,Hwang PP

Sat Apr 01 00:00:00 EDT 2023

Teleostean fishes may have developed an efficient Na⁺ uptake for adaptation to the freshwater system.

Frontiers in physiology

Tseng YC,Yan JJ,Furukawa F,Chen RD,Lee JR,Tsou YL,Liu TY,Tang YH,Hwang PP

Tue Oct 25 00:00:00 EDT 2022

Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency.

Autophagy

Wang F,Yang Y,Boudagh G,Eskelinen EL,Klionsky DJ,Malek SN

Mon Aug 01 00:00:00 EDT 2022

Inhibition of High-Temperature Requirement Protein A2 Protease Activity Represses Myogenic Differentiation via UPRmt.

International journal of molecular sciences

Sun H,Shen L,Zhang P,Lin F,Ma J,Wu Y,Yu H,Sun L

Tue Oct 04 00:00:00 EDT 2022

Toosendanin, a novel potent vacuolar-type H⁺-translocating ATPase inhibitor, sensitizes cancer cells to chemotherapy by blocking protective autophagy.

International journal of biological sciences

Dong Y,Zhu G,Wang SF,Keon KA,Rubinstein JL,Zeng SX,Zhang S,Chen QL,Fu J,Li M,Shen HM,Lu JJ,Chen XP,Lu JH

Thu May 12 00:00:00 EDT 2022

Toosendanin, a novel potent vacuolar-type H⁺-translocating ATPase inhibitor, sensitizes cancer cells to chemotherapy by blocking protective autophagy.

International journal of biological sciences

Dong Y,Zhu G,Wang SF,Keon KA,Rubinstein JL,Zeng SX,Zhang S,Chen QL,Fu J,Li M,Shen HM,Lu JJ,Chen XP,Lu JH

Thu May 12 00:00:00 EDT 2022

In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish.

International journal of molecular sciences

Shih SW,Yan JJ,Tsou YL,Lu SW,Wang MC,Chou MY,Hwang PP

Sat Apr 16 00:00:00 EDT 2022

In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish.

International journal of molecular sciences

Shih SW,Yan JJ,Tsou YL,Lu SW,Wang MC,Chou MY,Hwang PP

Sat Apr 16 00:00:00 EDT 2022

Tfeb-Mediated Transcriptional Regulation of Autophagy Induces Autosis during Ischemia/Reperfusion in the Heart.

Cells

Nah J,Sung EA,Zhai P,Zablocki D,Sadoshima J

Thu Jan 13 00:00:00 EST 2022

DNA-Unresponsive Platinum(II) Complex Induces ERS-Mediated Mitophagy in Cancer Cells.

Journal of medicinal chemistry

Guo Y,Jin S,Yuan H,Yang T,Wang K,Guo Z,Wang X

Thu Jan 13 00:00:00 EST 2022

Zinc enhances autophagic flux and lysosomal function through transcription factor EB activation and V-ATPase assembly.

Frontiers in cellular neuroscience

Kim KR,Park SE,Hong JY,Koh JY,Cho DH,Hwang JJ,Kim YH

Wed Oct 19 00:00:00 EDT 2022

Inhibition of human peptide deformylase by actinonin sensitizes glioblastoma cells to temozolomide chemotherapy.

Experimental cell research

Lan B,Zhao H,He Y,Zhao Z,Wang N,Gao Y

Tue Nov 15 00:00:00 EST 2022

Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.

Cell

Daniloski Z,Jordan TX,Wessels HH,Hoagland DA,Kasela S,Legut M,Maniatis S,Mimitou EP,Lu L,Geller E,Danziger O,Rosenberg BR,Phatnani H,Smibert P,Lappalainen T,tenOever BR,Sanjana NE

Thu Jan 07 00:00:00 EST 2021

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Cellular and molecular life sciences : CMLS

Castroflorio E,den Hoed J,Svistunova D,Finelli MJ,Cebrian-Serrano A,Corrochano S,Bassett AR,Davies B,Oliver PL

Thu Apr 01 00:00:00 EDT 2021

Ajugol enhances TFEB-mediated lysosome biogenesis and lipophagy to alleviate non-alcoholic fatty liver disease.

Pharmacological research

Zhang H,Lu J,Liu H,Guan L,Xu S,Wang Z,Qiu Y,Liu H,Peng L,Men X

Wed Dec 01 00:00:00 EST 2021

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.

Microorganisms

Yuan L,Chu B,Chen S,Li Y,Liu N,Zhu Y,Zhou D

Tue Nov 16 00:00:00 EST 2021

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Cell death & disease

Cao Y,Li R,Shen M,Li C,Zou Y,Jiang Q,Liu S,Lu C,Li H,Liu H,Cai Y

Tue Apr 20 00:00:00 EDT 2021

Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts.

Disease models & mechanisms

Santra P,Amack JD

Thu Jul 01 00:00:00 EDT 2021

Loss of vacuolar-type H+-ATPase induces caspase-independent necrosis-like death of hair cells in zebrafish neuromasts.

Disease models & mechanisms

Santra P,Amack JD

Thu Jul 01 00:00:00 EDT 2021

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhong B,Liu M,Bai C,Ruan Y,Wang Y,Qiu L,Hong Y,Wang X,Li L,Li B

Wed Apr 08 00:00:00 EDT 2020

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Veterinary research

Chu B,Zhu Y,Su J,Xia B,Zou Y,Nie J,Zhang W,Wang J

Wed Aug 05 00:00:00 EDT 2020

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells.

Journal of cellular physiology

Rao VK,Zavala G,Deb Roy A,Mains RE,Eipper BA

Sat Jun 01 00:00:00 EDT 2019

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.

Molecular & cellular proteomics : MCP

Pauwels AM,Härtlova A,Peltier J,Driege Y,Baudelet G,Brodin P,Trost M,Beyaert R,Hoffmann E

Wed May 01 00:00:00 EDT 2019

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Nature microbiology

Doyle T,Moncorgé O,Bonaventure B,Pollpeter D,Lussignol M,Tauziet M,Apolonia L,Catanese MT,Goujon C,Malim MH

Sat Dec 01 00:00:00 EST 2018

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Nature microbiology

Doyle T,Moncorgé O,Bonaventure B,Pollpeter D,Lussignol M,Tauziet M,Apolonia L,Catanese MT,Goujon C,Malim MH

Sat Dec 01 00:00:00 EST 2018

Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.

Molecular psychiatry

Pérez-Cañamás A,Benvegnù S,Rueda CB,Rábano A,Satrústegui J,Ledesma MD

Mon May 01 00:00:00 EDT 2017

Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.

Molecular psychiatry

Pérez-Cañamás A,Benvegnù S,Rueda CB,Rábano A,Satrústegui J,Ledesma MD

Mon May 01 00:00:00 EDT 2017

Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.

Molecular psychiatry

Pérez-Cañamás A,Benvegnù S,Rueda CB,Rábano A,Satrústegui J,Ledesma MD

Mon May 01 00:00:00 EDT 2017

Mycobacterium tuberculosis Controls Phagosomal Acidification by Targeting CISH-Mediated Signaling.

Cell reports

Queval CJ,Song OR,Carralot JP,Saliou JM,Bongiovanni A,Deloison G,Deboosère N,Jouny S,Iantomasi R,Delorme V,Debrie AS,Park SJ,Gouveia JC,Tomavo S,Brosch R,Yoshimura A,Yeramian E,Brodin P

Tue Sep 26 00:00:00 EDT 2017

Mycobacterium tuberculosis Controls Phagosomal Acidification by Targeting CISH-Mediated Signaling.

Cell reports

Queval CJ,Song OR,Carralot JP,Saliou JM,Bongiovanni A,Deloison G,Deboosère N,Jouny S,Iantomasi R,Delorme V,Debrie AS,Park SJ,Gouveia JC,Tomavo S,Brosch R,Yoshimura A,Yeramian E,Brodin P

Tue Sep 26 00:00:00 EDT 2017

Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.

Nature immunology

Martins R,Maier J,Gorki AD,Huber KV,Sharif O,Starkl P,Saluzzo S,Quattrone F,Gawish R,Lakovits K,Aichinger MC,Radic-Sarikas B,Lardeau CH,Hladik A,Korosec A,Brown M,Vaahtomeri K,Duggan M,Kerjaschki D,Esterbauer H,Colinge J,Eisenbarth SC,Decker T,Bennett KL,Kubicek S,Sixt M,Superti-Furga G,Knapp S

Thu Dec 01 00:00:00 EST 2016

Proton pump inhibitors protect mice from acute systemic inflammation and induce long-term cross-tolerance.

Cell death & disease

Balza E,Piccioli P,Carta S,Lavieri R,Gattorno M,Semino C,Castellani P,Rubartelli A

Thu Jul 21 00:00:00 EDT 2016

The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish.

Developmental biology

Gokey JJ,Dasgupta A,Amack JD

Sun Nov 01 00:00:00 EDT 2015

The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish.

Developmental biology

Gokey JJ,Dasgupta A,Amack JD

Sun Nov 01 00:00:00 EDT 2015

Rabconnectin3α promotes stable activity of the H+ pump on synaptic vesicles in hair cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Einhorn Z,Trapani JG,Liu Q,Nicolson T

Wed Aug 08 00:00:00 EDT 2012

Rabconnectin3α promotes stable activity of the H+ pump on synaptic vesicles in hair cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Einhorn Z,Trapani JG,Liu Q,Nicolson T

Wed Aug 08 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 70-kDa subunit; ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha 70 kDa, isoform 2; ATPase, H+ transporting, lysosomal 70kD, V1 subunit A, isoform 1; ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A; ATPase, H+ transporting, lysosomal, subunit A1; ATPase, H+ transporting, V1 subunit A, isoform 1; ATPase, H+ transporting, V1 subunit A1; H(+)-transporting two-sector ATPase, subunit A; H+-transporting ATPase chain A, vacuolar (VA68 type); lysosomal 70kDa; V-ATPase 69 kDa subunit; V-ATPase 69 kDa subunit 1; V-ATPase A subunit 1; V-ATPase subunit A; V-type proton ATPase catalytic subunit A; vacuolar ATP synthase catalytic subunit A, ubiquitous isoform; Vacuolar ATPase isoform VA68; vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha

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Gene Aliases: AI647066; ATP6A1; Atp6a2; ATP6V1A; ATP6V1A1; HO68; VA68; Vma1; VPP2

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UniProt ID: (Human) P38606, (Mouse) P50516

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Entrez Gene ID: (Human) 523, (Mouse) 11964, (Rat) 685232

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Function(s)

ATP binding proton-transporting ATPase activity, rotational mechanism nucleotide binding hydrolase activity hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ATP synthase primary active transporter transporter

Process(es)

transport insulin receptor signaling pathway ATP hydrolysis coupled proton transport regulation of macroautophagy transferrin transport ion transmembrane transport ATP metabolic process phagosome acidification ion transport proton transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP6V1A Polyclonal Antibody

ATP6V1A Antibody (17115-1-AP) in IHC (P)

Product Details

17115-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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