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FIGURE: 1 / 1
ATR Antibody (MA5-55249) in WB
Western Blot analysis of various lysates using ATR Recombinant Monoclonal Antibody (Product # MA5-55249) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55249
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4O3E0
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 2100-2200 of human ATR.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: KAYEWEKAGR SDRVQMRNDL GKINKVITEH TNYLAPYQFL TAFSQLISRI CHSHDEVFVV LMEIIAKVFL AYPQQAMWMM TAVSKSSYPM RVNRCKEILN K
Target Information
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8
Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q13535
Entrez Gene ID: (Human) 545, (Mouse) 245000
DNA binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
MutLalpha complex binding
MutSalpha complex binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
protein dimerization activity
non-receptor serine/threonine protein kinase
protein modifying enzyme
DNA damage checkpoint
DNA replication
DNA repair
cellular response to DNA damage stimulus
cell cycle
multicellular organism development
negative regulation of DNA replication
peptidyl-serine phosphorylation
positive regulation of telomere maintenance via telomerase
cellular response to UV
interstrand cross-link repair
response to drug
positive regulation of DNA damage response, signal transduction by p53 class mediator
protein autophosphorylation
protein localization to chromosome, telomeric region
cellular response to gamma radiation
replicative senescence
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
positive regulation of telomerase catalytic core complex assembly
double-strand break repair via homologous recombination
protein phosphorylation
phosphorylation
regulation of protein binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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