Proteintech

AXIN2 Polyclonal Antibody

AXIN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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AXIN2 Antibody (20540-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 20540-1-AP) (AXIN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

20540-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AXIN2 Fusion Protein Ag14574 (30-83 aa encoded by BC006295)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

20540-1-AP is a rabbit polyclonal antibody raised against a 34 amino acid N-terminal of human AXIN2. Observed MW of AXIN2 is from 93-100 kDa.

Immunogen sequence: EGETPPCQP GVGKGQVTKP MPVSSNTRRN EDGLGEPEGR ASPDSPLTRW TKSLH (30-83 aa encoded by BC006295)

Target Information

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin.

Signal transduction and targeted therapy

Zhou C,Zhu X,Liu N,Dong X,Zhang X,Huang H,Tang Y,Liu S,Hu M,Wang M,Deng X,Li S,Zhang R,Huang Y,Lyu H,Xiao S,Luo S,Ali DW,Michalak M,Chen XZ,Wang Z,Tang J

Fri Feb 17 00:00:00 EST 2023

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin.

Signal transduction and targeted therapy

Zhou C,Zhu X,Liu N,Dong X,Zhang X,Huang H,Tang Y,Liu S,Hu M,Wang M,Deng X,Li S,Zhang R,Huang Y,Lyu H,Xiao S,Luo S,Ali DW,Michalak M,Chen XZ,Wang Z,Tang J

Fri Feb 17 00:00:00 EST 2023

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin.

Signal transduction and targeted therapy

Zhou C,Zhu X,Liu N,Dong X,Zhang X,Huang H,Tang Y,Liu S,Hu M,Wang M,Deng X,Li S,Zhang R,Huang Y,Lyu H,Xiao S,Luo S,Ali DW,Michalak M,Chen XZ,Wang Z,Tang J

Fri Feb 17 00:00:00 EST 2023

mcPGK1-dependent mitochondrial import of PGK1 promotes metabolic reprogramming and self-renewal of liver TICs.

Nature communications

Chen Z,He Q,Lu T,Wu J,Shi G,He L,Zong H,Liu B,Zhu P

Mon Feb 27 00:00:00 EST 2023

Frequent PIK3CA mutation in normal endometrial gland drives spheroid formation and may be involved in stem cell propagation.

Cancer science

Sato S,Nakayama K,Kanno K,Sultana R,Ishikawa M,Ishibashi T,Yamashita H,Kyo S

Thu Jun 01 00:00:00 EDT 2023

Chebulagic acid suppresses gastric cancer by inhibiting the AURKA/β-catenin/Wnt pathway.

Frontiers in pharmacology

Zhao J,Shi Y,Ma Y,Pan L,Wang Y,Yuan L,Dong J,Ying J

Tue Mar 21 00:00:00 EDT 2023

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Kim H,Villareal LB,Liu Z,Haneef M,Falcon DM,Martin DR,Lee HJ,Dame MK,Attili D,Chen Y,Varani J,Spence JR,Kovbasnjuk O,Colacino JA,Lyssiotis CA,Lin HC,Shah YM,Xue X

Sat Apr 01 00:00:00 EDT 2023

Identification of a novel Calpain-2-SRC feed-back loop as necessity for β-Catenin accumulation and signaling activation in hepatocellular carcinoma.

Oncogene

Ma XL,Zhu KY,Chen YD,Tang WG,Xie SH,Zheng H,Tong Y,Wang YC,Ren N,Guo L,Lu RQ

Fri Jul 01 00:00:00 EDT 2022

Huoxin pill prevents excessive inflammation and cardiac dysfunction following myocardial infarction by inhibiting adverse Wnt/β‑catenin signaling activation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

He J,Wo D,Ma E,Wang Q,Chen J,Gao Q,Zhao Q,Shen F,Peng J,Zhu W,Ren DN

Thu Sep 01 00:00:00 EDT 2022

Efficacy of auranofin as an inhibitor of desmoid progression.

Scientific reports

Ito K,Nishida Y,Hamada S,Shimizu K,Sakai T,Ohkawara B,Alman BA,Enomoto A,Ikuta K,Koike H,Zhang J,Ohno K,Imagama S

Wed Jul 13 00:00:00 EDT 2022

PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.

Frontiers in cell and developmental biology

Liao S,Wang K,Zhang L,Shi G,Wang Z,Chen Z,Zhu P,He Q

Sat Jul 16 00:00:00 EDT 2022

Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.

Evidence-based complementary and alternative medicine : eCAM

Yang X,Luo G,Fu L,Huang H,Wang L,Yin L,Zhang X,Wang T,Ma X,Feng T,Ye J

Sat Jul 16 00:00:00 EDT 2022

Kangxianruangan granule‑containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt‑1/β‑catenin signaling pathway.

Molecular medicine reports

Tang W,Xue J,Luo L,Wang Y,Cai X,Liu Y,Huang D,Wang X,He T,Lu D,Yang F

Tue Feb 01 00:00:00 EST 2022

Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.

Food & function

Yang S,Shang J,Liu L,Tang Z,Meng X

Mon Nov 28 00:00:00 EST 2022

An SETD1A/Wnt/β-catenin feedback loop promotes NSCLC development.

Journal of experimental & clinical cancer research : CR

Wang R,Liu J,Li K,Yang G,Chen S,Wu J,Xie X,Ren H,Pang Y

Wed Oct 13 00:00:00 EDT 2021

Ultrathin 2D Titanium Carbide MXene (Ti₃ C₂ T_x ) Nanoflakes Activate WNT/HIF-1α-Mediated Metabolism Reprogramming for Periodontal Regeneration.

Advanced healthcare materials

Cui D,Kong N,Ding L,Guo Y,Yang W,Yan F

Mon Nov 01 00:00:00 EDT 2021

Babao Dan is a robust anti-tumor agent via inhibiting wnt/β-catenin activation and cancer cell stemness.

Journal of ethnopharmacology

Xie X,Chen J,Wo D,Ma E,Ning Y,Peng J,Zhu W,Ren DN

Mon Nov 15 00:00:00 EST 2021

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Autophagy

Zhou C,Liang Y,Zhou L,Yan Y,Liu N,Zhang R,Huang Y,Wang M,Tang Y,Ali DW,Wang Y,Michalak M,Chen XZ,Tang J

Fri Oct 01 00:00:00 EDT 2021

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 EDT 2021

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 EDT 2021

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 EDT 2021

AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).

International journal of molecular sciences

Zhang R,Yu S,Shen Q,Zhao W,Zhang J,Wu X,Zhu Z,Wu X,Li N,Peng S,Hua J

Tue Nov 30 00:00:00 EST 2021

Long Non-Coding RNA THOR Enhances the Stem Cell-Like Traits of Triple-Negative Breast Cancer Cells Through Activating β-Catenin Signaling.

Medical science monitor : international medical journal of experimental and clinical research

Wang B,Ye Q,Zou C

Tue Jul 14 00:00:00 EDT 2020

LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes.

Molecular cancer

Zhou C,Yi C,Yi Y,Qin W,Yan Y,Dong X,Zhang X,Huang Y,Zhang R,Wei J,Ali DW,Michalak M,Chen XZ,Tang J

Wed Jul 29 00:00:00 EDT 2020

TRIB3 confers glioma cell stemness via interacting with β-catenin.

Environmental toxicology

Lu Y,Li L,Chen L,Gao Y,Chen X,Cao Y

Mon Jun 01 00:00:00 EDT 2020

Oxaliplatin resistance is enhanced by saracatinib via upregulation Wnt-ABCG1 signaling in hepatocellular carcinoma.

BMC cancer

Liao X,Song G,Xu Z,Bu Y,Chang F,Jia F,Xiao X,Ren X,Zhang M,Jia Q

Mon Jan 13 00:00:00 EST 2020

TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.

OncoTargets and therapy

Zhang X,Luo Y,Li Q

Fri Apr 15 00:00:00 EDT 2022

[PARP10 Influences the Proliferation of Colorectal Carcinoma Cells, a Preliminary Study].

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Wu CF,Xiao M,Wang YL,Threadgill MD,Li M,Tang Y,Lin X,Yang L,Li QS,Li X

Wed Aug 19 00:00:00 EDT 2020

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

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Ma Z,Han C,Xia W,Wang S,Li X,Fang P,Yin R,Xu L,Yang L

Mon May 11 00:00:00 EDT 2020

Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer.

Theranostics

Zhang J,Ying Y,Li M,Wang M,Huang X,Jia M,Zeng J,Ma C,Zhang Y,Li C,Wang X,Shu XS

Fri May 28 00:00:00 EDT 2021

Hierarchical activation of compartmentalized pools of AMPK depends on severity of nutrient or energy stress.

Cell research

Zong Y,Zhang CS,Li M,Wang W,Wang Z,Hawley SA,Ma T,Feng JW,Tian X,Qi Q,Wu YQ,Zhang C,Ye Z,Lin SY,Piao HL,Hardie DG,Lin SC

Sat Jun 01 00:00:00 EDT 2019

MIR17HG-miR-18a/19a axis, regulated by interferon regulatory factor-1, promotes gastric cancer metastasis via Wnt/β-catenin signalling.

Cell death & disease

Yuan J,Tan L,Yin Z,Zhu W,Tao K,Wang G,Shi W,Gao J

Tue Jun 11 00:00:00 EDT 2019

Design, synthesis and biological evaluation of benzoylacrylic acid shikonin ester derivatives as irreversible dual inhibitors of tubulin and EGFR.

Bioorganic & medicinal chemistry

Sun WX,Han HW,Yang MK,Wen ZL,Wang YS,Fu JY,Lu YT,Wang MY,Bao JX,Lu GH,Qi JL,Wang XM,Lin HY,Yang YH

Sun Dec 01 00:00:00 EST 2019

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Nature cell biology

Zhu P,Wu J,Wang Y,Zhu X,Lu T,Liu B,He L,Ye B,Wang S,Meng S,Fan D,Wang J,Yang L,Qin X,Du Y,Li C,He L,Ren W,Wu X,Tian Y,Fan Z

Mon Oct 01 00:00:00 EDT 2018

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Nature cell biology

Zhu P,Wu J,Wang Y,Zhu X,Lu T,Liu B,He L,Ye B,Wang S,Meng S,Fan D,Wang J,Yang L,Qin X,Du Y,Li C,He L,Ren W,Wu X,Tian Y,Fan Z

Mon Oct 01 00:00:00 EDT 2018

Secreted AGR2 promotes invasion of colorectal cancer cells via Wnt11-mediated non-canonical Wnt signaling.

Experimental cell research

Tian S,Hu J,Tao K,Wang J,Chu Y,Li J,Liu Z,Ding X,Xu L,Li Q,Cai M,Gao J,Shuai X,Wang G,Wang L,Wang Z

Thu Mar 15 00:00:00 EDT 2018

Secreted AGR2 promotes invasion of colorectal cancer cells via Wnt11-mediated non-canonical Wnt signaling.

Experimental cell research

Tian S,Hu J,Tao K,Wang J,Chu Y,Li J,Liu Z,Ding X,Xu L,Li Q,Cai M,Gao J,Shuai X,Wang G,Wang L,Wang Z

Thu Mar 15 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Axil; Axin like protein; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582

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Gene Aliases: Axi1; AXIL; AXIN2; Conductin; ODCRCS

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UniProt ID: (Human) Q9Y2T1, (Mouse) O88566, (Rat) O70240

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Entrez Gene ID: (Human) 8313, (Mouse) 12006, (Rat) 29134

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Function(s)

GTPase activator activity protein binding beta-catenin binding enzyme binding protein kinase binding ubiquitin protein ligase binding armadillo repeat domain binding I-SMAD binding scaffold/adaptor protein

Process(es)

positive regulation of protein phosphorylation intramembranous ossification secondary heart field specification chondrocyte differentiation involved in endochondral bone morphogenesis regulation of transcription, DNA-templated cell proliferation negative regulation of cell proliferation dorsal/ventral axis specification positive regulation of epithelial to mesenchymal transition positive regulation of cell death Wnt signaling pathway bone mineralization regulation of mismatch repair cellular protein localization negative regulation of catenin import into nucleus odontogenesis positive regulation of GTPase activity maintenance of DNA repeat elements negative regulation of osteoblast differentiation mRNA stabilization regulation of chondrocyte development regulation of centromeric sister chromatid cohesion cellular response to organic cyclic compound negative regulation of canonical Wnt signaling pathway Wnt signaling pathway involved in somitogenesis positive regulation of canonical Wnt signaling pathway beta-catenin-TCF complex assembly somitogenesis cell death regulation of Wnt signaling pathway regulation of catenin import into nucleus termination of G-protein coupled receptor signaling pathway positive regulation of protein kinase activity negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AXIN2 Polyclonal Antibody

AXIN2 Antibody (20540-1-AP) in IHC (P)

Product Details

20540-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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