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AXIN2 Antibody (PA5-78247) in IHC (P)
axin 2 antibody [N2C2], Internal detects axin 2 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. Axin 2 antibody [N2C2], Internal (Product # PA5-78247) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-78247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Axin 2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736086
Product Specific Information
Predicted Reactivity: Bovine (80%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Axil; Axin like protein; Axin-2; Axin-like protein; Axis inhibition protein 2; Conductin; DKFZp781B0869; MGC10366; MGC126582
Gene Aliases: Axi1; AXIL; AXIN2; Conductin; ODCRCS
UniProt ID: (Human) Q9Y2T1, (Mouse) O88566, (Rat) O70240
Entrez Gene ID: (Human) 8313, (Mouse) 12006, (Rat) 29134
positive regulation of protein phosphorylation
intramembranous ossification
secondary heart field specification
chondrocyte differentiation involved in endochondral bone morphogenesis
regulation of transcription, DNA-templated
cell proliferation
negative regulation of cell proliferation
dorsal/ventral axis specification
positive regulation of epithelial to mesenchymal transition
positive regulation of cell death
Wnt signaling pathway
bone mineralization
regulation of mismatch repair
cellular protein localization
negative regulation of catenin import into nucleus
odontogenesis
positive regulation of GTPase activity
maintenance of DNA repeat elements
negative regulation of osteoblast differentiation
mRNA stabilization
regulation of chondrocyte development
regulation of centromeric sister chromatid cohesion
cellular response to organic cyclic compound
negative regulation of canonical Wnt signaling pathway
Wnt signaling pathway involved in somitogenesis
positive regulation of canonical Wnt signaling pathway
beta-catenin-TCF complex assembly
somitogenesis
cell death
regulation of Wnt signaling pathway
regulation of catenin import into nucleus
termination of G-protein coupled receptor signaling pathway
positive regulation of protein kinase activity
negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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