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AZIN1 Antibody (CF810899) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AZIN1 (RC200022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-AZIN1. Positive lysates LY402474 (100 µg) and LC402474 (20 µg) can be purchased separately from OriGene.
Product Details
CF810899
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1G8
Immunogen
Human recombinant protein fragment corresponding to amino acids 260-448 of human AZIN1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Antizyme inhibitor (AZIN1) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antizyme inhibitor 1; AZI; O OAZIN; ODC antizyme inhibitor; Ornithine decarboxylase antizyme inhibitor
Gene Aliases: 1700085L02Rik; AZI; AZIA1; AZIN1; OAZI; OAZIN; ODC1L
UniProt ID: (Human) O14977, (Mouse) O35484
Entrez Gene ID: (Human) 51582, (Rat) 58961, (Mouse) 54375
regulation of cellular amino acid metabolic process
putrescine biosynthetic process from ornithine
negative regulation of protein catabolic process
positive regulation of catalytic activity
positive regulation of polyamine transmembrane transport
polyamine biosynthetic process
negative regulation of catalytic activity
polyamine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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