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Abi1 Antibody (600-401-GT5) in WB
Western Blot of Rabbit anti-Abi1 antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: MEF3 KO. Lane 2: MEF3 WT. Lane 3: Mouse brain. Lane 4: Rat brain. Lane 5: HCT-116 WCL. Lane 6: HeLa WCL. Lane 7: NIH/3T3 WCL. Load: 35 µg per lane. Primary antibody: Abi1 antibody at 1:1,000 for o/n at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 65 kDa for Abi1.
Product Details
600-401-GT5
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Abi1 is directed against mouse Abi1. A BLAST analysis was used to suggest cross reactivity with this protein in human and rat based on 100% homology for the immunogen sequence. Reactivity against homologues from other sources is not known.
Target Information
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Abelson interactor 1; Abi-1; Abl interactor 1; Ablphilin-1; e3B1; EL2; eps8 binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; HS3; spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SPH3; SPTA
Gene Aliases: Abi1; E3B1; NAP1; Ssh3bp1
UniProt ID: (Mouse) Q8CBW3
Entrez Gene ID: (Mouse) 11308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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