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Invitrogen
Acetylated Lysine Monoclonal Antibody (1C6)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 14
Acetylated Lysine Antibody (MA1-2021) in ChIP
Chromatin immunoprecipitation analysis (ChIP) of chromatin proteins with acetylated lysines was performed using cross-linked chromatin from 4x10^6 LNCaP cells. Immunoprecipitation was performed using the Pierce Magnetic ChIP kit (Product # 26157) with 10 µg of an Acetyl Lysine monoclonal antibody (Product # MA1-2021). Quantitative real-time PCR data was obtained using a Thermo PikoReal PCR System (Product # TCR0096), SYBR Green PCR Master Mix, and primers (5’-TACTAGCGGTTTTACGGGCG, 3’-TCGAACAGGAGGAGCAGAGAGCGA) flanking the human GAPDH promo... View More
Product Details
MA1-2021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1C6
Immunogen
Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2257634
Product Specific Information
MA1-2021 detects human and mouse, and monkey acetylated lysine.
MA1-2021 has been used successfully in Western blot, immunoprecipitation, dot blot, ChIP Assay, and immunofluorescence (cells) procedures. By Western blot, this antibody may detect a ladder of acetylated proteins in HeLa cell lysate. In immunofluorescence procedures, MA1-2021 recognizes acetylated lysine in gamma irradiated HeLa cells.
The MA1-2021 immunogen is a synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.
Target Information
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acetyl Lysine; Acetylated Lysine; Acetyllysine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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