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OriGene
Acidic Cytokeratin Monoclonal Antibody (OTI8A3), TrueMAB™
Product Details
CF190228
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI8A3
Immunogen
Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20).
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Synthetic peptide sequence is EKVTMQNLND RLASYLDKVR ALEEAN
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; Acidic Cytokeratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-16; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; Cytokeratin-10; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-9; focal non-epidermolytic palmoplantar keratoderma; HMWCK; K10; K14; K15; K16; K18; K19; K9; keratin 10, type I; keratin 14, type I; keratin 15, type I; keratin 16, type I; keratin 18, type I; keratin 19, type I; keratin 9, type I; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; Keratin-10; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-9; pan keratin; pankeratin; type I cytoskeletal 15; type I cytoskeletal 9
Gene Aliases: BCIE; BIE; CK-18; CK-9; CK10; CK14; CK15; CK16; CK19; CYK18; EBS3; EBS4; EHK; EPPK; FNEPPK; K10; K14; K15; K16; K18; K19; K1CO; K1CP; K1CS; K9; KPP; KRT10; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT9; KRTB; NEPPK; NFJ; PC1; PIG46
UniProt ID: (Human) P13645, (Human) P02533, (Human) P19012, (Human) P08779, (Human) P05783, (Human) P08727, (Human) P35527
Entrez Gene ID: (Human) 3858, (Human) 3861, (Human) 3866, (Human) 3868, (Human) 3875, (Human) 3880, (Human) 3857
cytoskeleton organization
epidermis development
mitophagy in response to mitochondrial depolarization
Notch signaling pathway
viral process
response to estrogen
sarcomere organization
cell differentiation involved in embryonic placenta development
aging
response to zinc ion
response to ionizing radiation
epithelial cell differentiation
hemidesmosome assembly
hair cycle
intermediate filament bundle assembly
keratinocyte differentiation
cell cycle
anatomical structure morphogenesis
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cell-cell adhesion
spermatogenesis
skin development
intermediate filament organization
morphogenesis of an epithelium
inflammatory response
cell proliferation
negative regulation of cell migration
keratinization
innate immune response
keratinocyte migration
establishment of skin barrier
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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