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Invitrogen
Aconitase 2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 15
Aconitase 2 Antibody (PA5-29960) in ICC/IF
Immunofluorescence analysis of Aco2; Aconitase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Aconitase 2 Polyclonal Antibody (Product # PA5-29960) at 1:200 dilution in 0.1% BSA, incubated at 4 deg... View More
Product Details
PA5-29960
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 74 and 340 of Human Aconitase 2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547434
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, JurKat, Mouse brain, rat brain.
Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (91%), Xenopus laevis (94%), Pig (98%), Chicken (95%), Rhesus Monkey (98%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aconitase; aconitase 2, mitochondrial; Aconitate hydratase, mitochondrial; Citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; MGC20605; MGC33908; mitochondrial aconitase; mitochondrial aconitase (nuclear aco2 gene); RP3-347H13.8
Gene Aliases: Aco-2; ACO2; Aco3; ACONM; D10Wsu183e; HEL-S-284; ICRD; OCA8; OPA9
UniProt ID: (Human) Q99798, (Rat) Q9ER34, (Mouse) Q99KI0
Entrez Gene ID: (Chicken) 374009, (Human) 50, (Rat) 79250, (Mouse) 11429
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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