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Actin Antibody (MA5-55245) in WB
Western blot analysis of lysates from Oryza sativa, using Actin Recombinant Monoclonal Antibody (Product # MA5-55245) secondary antibody at 1:10,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-55245
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5M5Z8
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of Actin(Plant Specific).
Conjugate
Form
Liquid
Concentration
2.12 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-sma; Atcb; pan actin; RP5-1068B5.2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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