Invitrogen

Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

11 References

View all (5) Active/Pro-Caspase 3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Active/Pro-Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 21

Active/Pro-Caspase 3 Antibody (MA1-91637) in IHC (P)

Immunohistochemistry (Paraffin) analysis of Caspase 3 (active/pro) in human thymus tissue using Caspase 3 (active/pro) Monoclonal Antibody (31A1067) (Product # MA1-91637) at a dilution of 5 µg/mL. Antigen retrieval : Heat-induced antigen retrieval.

Active/Pro-Caspase 3 Antibody in Western Blot (WB)

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Product Details

MA1-91637

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

Immunomicroscopy (IM)

Assay-dependent

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

31A1067

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1954999

Product Specific Information

Predicted molecular weight: 31kDa.

Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thyroid hormone-induced cell death in sea urchin metamorphic development.

The Journal of experimental biology

Wynen H,Taylor E,Heyland A

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Immunohistochemistry (Paraffin)

Thu Dec 01 00:00:00 EST 2022

The protective effects of propofol against renal ischemia-reperfusion injury are potentiated by norisoboldine treatment via inhibition of oxidative stress pathways.

Journal of biochemical and molecular toxicology

Xing D,Li Q,Lin G,Lin H,Kang W,Zhang M,Ding R,Li N

MA1-91637 was used in Western Blotting to suggest that treatment with NOR potentiated the nephroprotective effects of propofol in rats with I/R-induced renal injury by ameliorating oxidative stress and apoptosis pathway.

Sat Jan 01 00:00:00 EST 2022

Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients.

Journal of cachexia, sarcopenia and muscle

Mallard J,Hucteau E,Charles AL,Bender L,Baeza C,Pélissie M,Trensz P,Pflumio C,Kalish-Weindling M,Gény B,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

MA1-91637 was used in Western Blotting to investigating overall skeletal muscle mitochondrial homeostasis in early breast cancer patients undergoing chemotherapy, including mitochondrial quantity, function, and dynamics.

Wed Jun 01 00:00:00 EDT 2022

Aging lens epithelium is susceptible to ferroptosis.

Free radical biology & medicine

Wei Z,Hao C,Huangfu J,Srinivasagan R,Zhang X,Fan X

MA1-91637 was used in Western Blotting to explore the lens epithelial cell's (LEC) susceptibility to ferroptosis after documentation has concluded that aged and cataractous human lenses manifest with increased reactive oxygen species (ROS) formation, elevated lipid peroxidation, and accumulative intracellular redox-active iron, constituting the three hallmarks of ferroptosis during aging and cataractogenesis.

Sat May 01 00:00:00 EDT 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

MA1-91637 was used in Immunohistochemistry-immunofluorescence to demonstrate that normally quiescent lens epithelial cells can be reactivated for epithelialization repair in response to UV-induced damage.

Wed Dec 01 00:00:00 EST 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

Wed Dec 01 00:00:00 EST 2021

Pristimerin inhibits neuronal inflammation and protects cognitive function in mice with sepsis-induced brain injuries by regulating PI3K/Akt signalling.

Pharmaceutical biology

Xue W,Li Y,Zhang M