Invitrogen

Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech®

View all (2) Adipolean antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

500-P193GBT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human gAcrp30/Adipolean
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929765

Product Specific Information

AA Sequence of recombinant protein: MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean. Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hAdipolean by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean (500-P193G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hAdipolean.

Western Blot: To detect Human Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adipolean is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P193GBT-1MG will be provided as 2 x 500 µg

Target Information

gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and named AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; Adipocyte complement-related 30 kDa protein; Adipocyte, C1q and collagen domain-containing protein; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; apM-1; gAcrp30; Gelatin-binding protein; gelatin-binding protein 28

View more View less

Gene Aliases: ACDC; ACRP30; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; GBP28

View more View less

UniProt ID: (Human) Q15848

View more View less

Entrez Gene ID: (Human) 9370

View more View less

Function(s)
receptor binding cytokine activity hormone activity protein binding sialic acid binding identical protein binding protein homodimerization activity
Process(es)
response to hypoxia positive regulation of protein phosphorylation glucose metabolic process generation of precursor metabolites and energy fatty acid beta-oxidation response to nutrient circadian rhythm response to sucrose response to glucose positive regulation of signal transduction negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of protein kinase A signaling negative regulation of macrophage derived foam cell differentiation negative regulation of tumor necrosis factor-mediated signaling pathway positive regulation of cholesterol efflux regulation of glucose metabolic process response to activity negative regulation of smooth muscle cell migration fatty acid oxidation negative regulation of cell migration negative regulation of granulocyte differentiation negative regulation of protein autophosphorylation positive regulation of cellular protein metabolic process negative regulation of tumor necrosis factor production positive regulation of interleukin-8 production cellular response to insulin stimulus positive regulation of myeloid cell apoptotic process adiponectin-activated signaling pathway negative regulation of heterotypic cell-cell adhesion low-density lipoprotein particle clearance response to tumor necrosis factor cellular response to drug glucose homeostasis positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAP kinase activity response to ethanol negative regulation of fat cell differentiation negative regulation of macrophage differentiation negative regulation of low-density lipoprotein particle receptor biosynthetic process negative regulation of gluconeogenesis negative regulation of blood pressure positive regulation of blood pressure negative regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of glucose import negative regulation of hormone secretion negative regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis negative regulation of synaptic transmission brown fat cell differentiation protein homooligomerization response to glucocorticoid membrane depolarization membrane hyperpolarization protein heterotrimerization negative regulation of ERK1 and ERK2 cascade response to linoleic acid detection of oxidative stress cellular response to cAMP positive regulation of monocyte chemotactic protein-1 production cellular response to epinephrine stimulus protein localization to plasma membrane negative regulation of intracellular protein transport negative regulation of receptor binding negative regulation of DNA biosynthetic process positive regulation of glycogen (starch) synthase activity positive regulation of metanephric glomerular visceral epithelial cell development positive regulation of cAMP-dependent protein kinase activity positive regulation of renal albumin absorption negative regulation of platelet-derived growth factor receptor-alpha signaling pathway negative regulation of metanephric mesenchymal cell migration
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support