Invitrogen

Adiponectin Monoclonal Antibody (19F1)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

19 Published Figures

25 References

View all (70) Adiponectin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Adiponectin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 27

Adiponectin Antibody (MA1-054) in ICC/IF

Immunofluorescence analysis of Adiponectin was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adiponectin (19F1) Mouse Monoclona... View More

Adiponectin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Adiponectin Antibody in Western Blot (WB)

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Product Details

MA1-054

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Bovine, Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

19F1

Immunogen

Full length, recombinant, human adiponectin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_557516

Product Specific Information

MA1-054 detects adiponectin from human, rabbit and mouse samples.

MA1-054 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~30 kDa protein representing Adiponectin in 3T3-L1 adipocytes. Immunofluorescence and ELISA of Adiponectin can also be performed using MA1-054.

The MA1-054 immunogen is full length, recombinant, human adiponectin.

Target Information

Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows.

Frontiers in cell and developmental biology

Michelotti TC,Kisby BR,Flores LS,Tegeler AP,Fokar M,Crasto C,Menarim BC,Loux SC,Strieder-Barboza C

MA1-054 was used in Immunohistochemistry (PFA fixed) and Immunocytochemistry to demonstrate cell-type and depot-specific heterogeneity in VAT and SAT of dairy cows.

Wed Nov 02 00:00:00 EDT 2022

Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows.

Frontiers in cell and developmental biology

Michelotti TC,Kisby BR,Flores LS,Tegeler AP,Fokar M,Crasto C,Menarim BC,Loux SC,Strieder-Barboza C

MA1-054 was used in Immunohistochemistry (PFA fixed) and Immunocytochemistry to demonstrate cell-type and depot-specific heterogeneity in VAT and SAT of dairy cows.

Wed Nov 02 00:00:00 EDT 2022

Single-nuclei analysis reveals depot-specific transcriptional heterogeneity and depot-specific cell types in adipose tissue of dairy cows.

Frontiers in cell and developmental biology

Michelotti TC,Kisby BR,Flores LS,Tegeler AP,Fokar M,Crasto C,Menarim BC,Loux SC,Strieder-Barboza C

MA1-054 was used in Immunohistochemistry (PFA fixed) and Immunocytochemistry to demonstrate cell-type and depot-specific heterogeneity in VAT and SAT of dairy cows.

Wed Nov 02 00:00:00 EDT 2022

SLC3A2 and SLC7A2 Mediate the Exogenous Putrescine-Induced Adipocyte Differentiation.

Molecules and cells

Eom J,Choi J,Suh SS,Seo JB

MA1-054 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that exogenous polyamines (such as putrescine) entering the adipocytes through polyamine transporters, can stimulate adipogenesis.

Sat Dec 31 00:00:00 EST 2022

SLC3A2 and SLC7A2 Mediate the Exogenous Putrescine-Induced Adipocyte Differentiation.

Molecules and cells

Eom J,Choi J,Suh SS,Seo JB

Sat Dec 31 00:00:00 EST 2022

Morphological and Immunophenotypical Changes of Human Bone Marrow Adipocytes in Marrow Metastasis and Myelofibrosis.

Frontiers in endocrinology

Dello Spedale Venti M,Palmisano B,Donsante S,Farinacci G,Adotti F,Coletta I,Serafini M,Corsi A,Riminucci M

MA1-054 was used in Immunohistochemistry to study in situ changes in both morphology and immunophenotype of bone marrow adipocytes (BMAds) under two different neoplastic marrow conditions: bone marrow metastasis (MET) and myeloproliferative neoplasia with grade-3 myelofibrosis (MPN-MF).

Tue Jun 28 00:00:00 EDT 2022

European journal of endocrinology

Sollier C,Capel E,Aguilhon C,Smirnov V,Auclair M,Douillard C,Ladsous M,Defoort-Dhellemmes S,Gorwood J,Braud L,Motterlini R,Vatier C,Lascols O,Renard E,Vigouroux C,Jéru I

MA1-054 was used in Western Blot to show that detailed ophthalmological examination could reveal retinal damage, further pointing to the nervous tissue as an important disease target.

Fri Jan 01 00:00:00 EST 2021

The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding.

Cell reports

Kuramoto K,Kim YJ,Hong JH,He C

MA1-054 was used in Western Blot to demonstrate that Becn1 improves insulin sensitivity by facilitating adiponectin secretion through binding the exocyst in adipose tissue.

Tue May 25 00:00:00 EDT 2021

The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding.

Cell reports

Kuramoto K,Kim YJ,Hong JH,He C

MA1-054 was used in Western Blot to demonstrate that Becn1 improves insulin sensitivity by facilitating adiponectin secretion through binding the exocyst in adipose tissue.

Tue May 25 00:00:00 EDT 2021

EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence.

eLife

Gautheron J,Morisseau C,Chung WK,Zammouri J,Auclair M,Baujat G,Capel E,Moulin C,Wang Y,Yang J,Hammock BD,Cerame B,Phan F,Fève B,Vigouroux C,Andreelli F,Jeru I

MA1-054 was used in Western Blot to highlight the importance of epoxide regulation for adipocyte function and provides new insights into the physiological roles of EHs in humans.

Tue Aug 03 00:00:00 EDT 2021

EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence.

eLife

Gautheron J,Morisseau C,Chung WK,Zammouri J,Auclair M,Baujat G,Capel E,Moulin C,Wang Y,Yang J,Hammock BD,Cerame B,Phan F,Fève B,Vigouroux C,Andreelli F,Jeru I

MA1-054 was used in Western Blot to highlight the importance of epoxide regulation for adipocyte function and provides new insights into the physiological roles of EHs in humans.

Tue Aug 03 00:00:00 EDT 2021

Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery.

Nature communications

Di Franco S,Bianca P,Sardina DS,Turdo A,Gaggianesi M,Veschi V,Nicotra A,Mangiapane LR,Lo Iacono M,Pillitteri I,van Hooff S,Martorana F,Motta G,Gulotta E,Lentini VL,Martorana E,Fiori ME,Vieni S,Bongiorno MR,Giannone G,Giuffrida D,Memeo L,Colarossi L,Mare M,Vigneri P,Todaro M,De Maria R,Medema JP,Stassi G

MA1-054 was used in Immunohistochemistry (Paraffin) to study the relation between obesity and cancer metastasis.

Wed Aug 18 00:00:00 EDT 2021

TAZ Is a Negative Regulator of PPARγ Activity in Adipocytes and TAZ Deletion Improves Insulin Sensitivity and Glucose Tolerance.

Cell metabolism

El Ouarrat D,Isaac R,Lee YS,Oh DY,Wollam J,Lackey D,Riopel M,Bandyopadhyay G,Seo JB,Sampath-Kumar R,Olefsky JM

MA1-054 was used in an ELISA assay to study the role of transcriptional co-activator with PDZ-binding motif (TAZ) as a transcriptional co-repressor of PPARγ and identify TAZ as a new factor in the development of obesity-induced insulin resistance.

Tue Jan 07 00:00:00 EST 2020

Site-Dependent Lineage Preference of Adipose Stem Cells.

Frontiers in cell and developmental biology

Wang T,Hill RC,Dzieciatkowska M,Zhu L,Infante AM,Hu G,Hansen KC,Pei M

MA1-054 was used in Western Blotting to investigate the variance of depot-specific adult stem cells from adipose tissues.

Wed Dec 14 00:00:00 EST 2022

Site-Dependent Lineage Preference of Adipose Stem Cells.

Frontiers in cell and developmental biology

Wang T,Hill RC,Dzieciatkowska M,Zhu L,Infante AM,Hu G,Hansen KC,Pei M

MA1-054 was used in Western Blotting to investigate the variance of depot-specific adult stem cells from adipose tissues.

Wed Dec 14 00:00:00 EST 2022

Bioprinted Skin Recapitulates Normal Collagen Remodeling in Full-Thickness Wounds.

Tissue engineering. Part A

Jorgensen AM,Varkey M,Gorkun A,Clouse C,Xu L,Chou Z,Murphy SV,Molnar J,Lee SJ,Yoo JJ,Soker S,Atala A

MA1-054 was used in Immunohistochemistry to demonstrate the utility of bioprinted skin for epidermal barrier formation and normal collagen remodeling in full-thickness wounds.

Fri May 01 00:00:00 EDT 2020

Knockdown of Ant2 Reduces Adipocyte Hypoxia And Improves Insulin Resistance in Obesity.

Nature metabolism

Seo JB,Riopel M,Cabrales P,Huh JY,Bandyopadhyay GK,Andreyev AY,Murphy AN,Beeman SC,Smith GI,Klein S,Lee YS,Olefsky JM

Published figure using Adiponectin monoclonal antibody (Product # MA1-054) in Western Blot

Tue Jan 01 00:00:00 EST 2019

CITED2 mediates the mechanical loading-induced suppression of adipokines in the infrapatellar fat pad.

Annals of the New York Academy of Sciences

Liu L,He Z,Xu L,Lu L,Feng H,Leong DJ,Kim SJ,Hirsh DM,Majeska RJ,Goldring MB,Cobelli NJ,Sun HB

MA1-054 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that CITED2 is a critical regulator in physiologic loading-induced chondroprotection in the context of a high-fat diet and PGC-1α is required for the inhibitory effects of CITED2 on the expression of adipokines in the infrapatellar fat pad.

Mon Apr 01 00:00:00 EDT 2019

Regulator of Calcineurin 1 helps coordinate whole-body metabolism and thermogenesis.

EMBO reports

Rotter D,Peiris H,Grinsfelder DB,Martin AM,Burchfield J,Parra V,Hull C,Morales CR,Jessup CF,Matusica D,Parks BW,Lusis AJ,Nguyen NUN,Oh M,Iyoke I,Jakkampudi T,McMillan DR,Sadek HA,Watt MJ,Gupta RK,Pritchard MA,Keating DJ,Rothermel BA

MA1-054 was used in Western Blotting to provide evidence that Regulator of Calcineurin 1 (RCAN1) acts to suppress two distinctly different mechanisms of non-shivering thermogenesis (NST).

Sat Dec 01 00:00:00 EST 2018

Regulator of Calcineurin 1 helps coordinate whole-body metabolism and thermogenesis.

EMBO reports

MA1-054 was used in Western Blotting to provide evidence that Regulator of Calcineurin 1 (RCAN1) acts to suppress two distinctly different mechanisms of non-shivering thermogenesis (NST).

Sat Dec 01 00:00:00 EST 2018

Prospective validation of a novel renal activity index of lupus nephritis.

Lupus

Gulati G,Bennett MR,Abulaban K,Song H,Zhang X,Ma Q,Brodsky SV,Nadasdy T,Haffner C,Wiley K,Ardoin SP,Devarajan P,Ying J,Rovin BH,Brunner HI

MA1-054 was used in Immunohistochemistry-immunofluorescence to validate the renal activity index for lupus in adults with lupus nephritis.

Tue Aug 01 00:00:00 EDT 2017

Expansion of Bone Marrow Adipose Tissue During Caloric Restriction Is Associated With Increased Circulating Glucocorticoids and Not With Hypoleptinemia.

Endocrinology

Cawthorn WP,Scheller EL,Parlee SD,Pham HA,Learman BS,Redshaw CM,Sulston RJ,Burr AA,Das AK,Simon BR,Mori H,Bree AJ,Schell B,Krishnan V,MacDougald OA

MA1-054 was used in western blot to test if caloric restriction affects adiponectin production by bone marrow adipose tissue

Mon Feb 01 00:00:00 EST 2016

Effect of 12 Weeks of Periodized Resistance Training Upon Total Plasma Adiponectin Concentration in Healthy Young Men.

Journal of strength and conditioning research

Davis GR,Stephens JM,Nelson AG

MA1-054 was used in western blot to determine the effect of periodized resistance training on plasma adiponectin

Sun Nov 01 00:00:00 EDT 2015

Bone marrow adipose tissue is an endocrine organ that contributes to increased circulating adiponectin during caloric restriction.

Cell metabolism

Cawthorn WP,Scheller EL,Learman BS,Parlee SD,Simon BR,Mori H,Ning X,Bree AJ,Schell B,Broome DT,Soliman SS,DelProposto JL,Lumeng CN,Mitra A,Pandit SV,Gallagher KA,Miller JD,Krishnan V,Hui SK,Bredella MA,Fazeli PK,Klibanski A,Horowitz MC,Rosen CJ,MacDougald OA

Published figure using Adiponectin monoclonal antibody (Product # MA1-054) in Western Blot

Tue Aug 05 00:00:00 EDT 2014

St. John's Wort Has Metabolically Favorable Effects on Adipocytes In Vivo.

Evidence-based complementary and alternative medicine : eCAM

Fuller S,Richard AJ,Ribnicky DM,Beyl R,Mynatt R,Stephens JM

MA1-054 was used in western blot to study the effects of St John's Wart on adipocyte function in vivo

Tue Aug 19 00:00:00 EDT 2014

Biochemical changes induced by strontium ranelate in differentiating adipocytes.

Biochimie

Vidal C,Gunaratnam K,Tong J,Duque G

MA1-054 was used in western blot to study the effect of the osteoporosis therapeutic strontium ranelate on differentiating adipocytes

Mon Apr 01 00:00:00 EDT 2013

Naringenin inhibits adipogenesis and reduces insulin sensitivity and adiponectin expression in adipocytes.

Evidence-based complementary and alternative medicine : eCAM

Richard AJ,Amini-Vaughan Z,Ribnicky DM,Stephens JM

MA1-054 was used in western blot to study the inhibition of adipogenesis and the decreased insulin sensitivity and adiponectin expression of adipocytes treated with naringenin

Wed Aug 28 00:00:00 EDT 2013

The transcription factor paired-related homeobox 1 (Prrx1) inhibits adipogenesis by activating transforming growth factor-β (TGFβ) signaling.

The Journal of biological chemistry

Du B,Cawthorn WP,Su A,Doucette CR,Yao Y,Hemati N,Kampert S,McCoin C,Broome DT,Rosen CJ,Yang G,MacDougald OA

MA1-054 was used in western blot to study the role of TGF-beta in the mechanism by which Prrx1 inhibits adipogenesis

Fri Feb 01 00:00:00 EST 2013

The transcription factor paired-related homeobox 1 (Prrx1) inhibits adipogenesis by activating transforming growth factor-β (TGFβ) signaling.

The Journal of biological chemistry

Du B,Cawthorn WP,Su A,Doucette CR,Yao Y,Hemati N,Kampert S,McCoin C,Broome DT,Rosen CJ,Yang G,MacDougald OA

MA1-054 was used in western blot to study the role of TGF-beta in the mechanism by which Prrx1 inhibits adipogenesis

Fri Feb 01 00:00:00 EST 2013

Snail, a transcriptional regulator, represses adiponectin expression by directly binding to an E-box motif in the promoter.

Metabolism: clinical and experimental

Park YM,Lee YH,Kim SH,Lee EY,Kim KS,Williams DR,Lee HC

MA1-054 was used in western blot to investigate the mechanism for the repressive effect of Snail on adiponectin expression

Thu Nov 01 00:00:00 EDT 2012

LTP induction translocates cortactin at distant synapses in wild-type but not Fmr1 knock-out mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Seese RR,Babayan AH,Katz AM,Cox CD,Lauterborn JC,Lynch G,Gall CM

MA1-054 was used in immunohistochemistry to study the effect of LTP induction on cortactin translocation in wild-type and fmr1 knock-out mice

Wed May 23 00:00:00 EDT 2012

HIV protease inhibitors activate the adipocyte renin angiotensin system.

Antiviral therapy

Boccara F,Auclair M,Cohen A,Lefèvre C,Prot M,Bastard JP,Capeau J,Caron-Debarle M

MA1-054 was used in western blot to investigate the effect of HIV protease inhibitors on the activation of adipose renin angiotensin system

Mon Sep 27 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; adipocyte complement related protein; Adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADPIOQ; apM-1; Gelatin-binding protein; gelatin-binding protein 28

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Gene Aliases: 30kDa; ACDC; ACRP30; Ad; adipo; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; APN; GBP28

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UniProt ID: (Human) Q15848, (Mouse) Q60994

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Entrez Gene ID: (Human) 9370, (Rabbit) 100009027, (Mouse) 11450

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Function(s)

receptor binding cytokine activity hormone activity protein binding sialic acid binding identical protein binding protein homodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Adiponectin Monoclonal Antibody (19F1)

Adiponectin Antibody (MA1-054) in ICC/IF

Product Details

MA1-054

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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