Proteintech

Adiponectin receptor Polyclonal Antibody

23 References

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Adiponectin receptor Antibody in Western Blot (WB)

FIGURE: 1 / 4

Adiponectin receptor Antibody (14361-1-AP) in WB

Mouse uterus tissue were subjected to SDS PAGE followed by western blot with 14361-1-AP (ADIPOR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Adiponectin receptor Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14361-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Hamster, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Adiponectin receptor Fusion Protein Ag5744 (1-147 aa encoded by BC051858)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.

Immunogen sequence: MNEPTENRL GCSRTPEPDI RLRKGHQLDG TRRGDNDSHQ GDLEPILEAS VLSSHHKKSS EEHEYSDEAP QEDEGFMGMS PLLQAHHAME KMEEFVCKVW EGRWRVIPHD VLPDWLKDND FLLHGHRPPM PSFRACFKSI FRIHTETG (1-147 aa encoded by BC051858)

Target Information

Adiponectin (also known as 30- kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR-. Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway.

Journal of agricultural and food chemistry

Tang C,Zhao H,Kong L,Meng F,Zhou L,Lu Z,Lu Y

Wed Jan 25 00:00:00 EST 2023

The Mef2c/AdipoR1 axis is responsible for myogenic differentiation and is regulated by resistin in skeletal muscles.

Gene

Wen F,Hou J,Ji X,Chu X,Liu X,Shi Z,Song Z

Mon Mar 20 00:00:00 EDT 2023

Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs.

Food & function

Gong S,Yin Y,Han M,Guo L,Duan Y,Guo Q,Yin J,Li F

Tue Jan 03 00:00:00 EST 2023

Advanced Glycosylation End Products Induced Synaptic Deficits and Cognitive Decline Through ROS-JNK-p53/miR-34c/SYT1 Axis in Diabetic Encephalopathy.

Journal of Alzheimer's disease : JAD

Zhang R,Jiang L,Li G,Wu J,Tian P,Zhang D,Qin Y,Shi Z,Gao Z,Zhang N,Wang S,Zhou H,Xu S

Tue May 24 00:00:00 EDT 2022

Advanced Glycosylation End Products Induced Synaptic Deficits and Cognitive Decline Through ROS-JNK-p53/miR-34c/SYT1 Axis in Diabetic Encephalopathy.

Journal of Alzheimer's disease : JAD

Zhang R,Jiang L,Li G,Wu J,Tian P,Zhang D,Qin Y,Shi Z,Gao Z,Zhang N,Wang S,Zhou H,Xu S

Tue May 24 00:00:00 EDT 2022

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

The EMBO journal

Volkmar N,Gawden-Bone CM,Williamson JC,Nixon-Abell J,West JA,St George-Hyslop PH,Kaser A,Lehner PJ

Tue Oct 04 00:00:00 EDT 2022

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

The EMBO journal

Volkmar N,Gawden-Bone CM,Williamson JC,Nixon-Abell J,West JA,St George-Hyslop PH,Kaser A,Lehner PJ

Tue Oct 04 00:00:00 EDT 2022

Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets.

Animal biotechnology

Chen X,Xiang L,Huang Z,Jia G,Liu G,Zhao H

Wed Jun 01 00:00:00 EDT 2022

Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.

Animal biotechnology

Xiang L,Huang Z,Chen X,Jia G,Liu G,Zhao H

Fri Apr 01 00:00:00 EDT 2022

D-chiro-Inositol facilitates adiponectin biosynthesis and activates the AMPKα/PPARs pathway to inhibit high-fat diet-induced obesity and liver lipid deposition.

Food & function

Yang Q,Zhang Y,Li L,Li J,Li Y,Han L,Wang M

Mon Jul 04 00:00:00 EDT 2022

Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.

Food & function

Chen X,Liang D,Huang Z,Jia G,Zhao H,Liu G

Sun Mar 21 00:00:00 EDT 2021

Carnosic acid alleviates depression-like behaviors on chronic mild stressed mice via PPAR-γ-dependent regulation of ADPN/FGF9 pathway.

Psychopharmacology

Wang XQ,Tang YH,Zeng GR,Wu LF,Zhou YJ,Cheng ZN,Jiang DJ

Mon Feb 01 00:00:00 EST 2021

Adiponectin Modulation by Genotype and Maternal Choline Supplementation in a Mouse Model of Down Syndrome and Alzheimer's Disease.

Journal of clinical medicine

Alldred MJ,Lee SH,Ginsberg SD

Mon Jul 05 00:00:00 EDT 2021

A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference.

Biology open

Graffmann N,Ncube A,Martins S,Fiszl AR,Reuther P,Bohndorf M,Wruck W,Beller M,Czekelius C,Adjaye J

Mon Jan 25 00:00:00 EST 2021

Adiponectin Suppresses Human Pancreatic Cancer Growth through Attenuating the β-Catenin Signaling Pathway.

International journal of biological sciences

Jiang J,Fan Y,Zhang W,Shen Y,Liu T,Yao M,Gu J,Tu H,Gan Y

Mon Jan 13 00:00:00 EST 2020

Adiponectin Suppresses Human Pancreatic Cancer Growth through Attenuating the β-Catenin Signaling Pathway.

International journal of biological sciences

Jiang J,Fan Y,Zhang W,Shen Y,Liu T,Yao M,Gu J,Tu H,Gan Y

Mon Jan 13 00:00:00 EST 2020

Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer.

Cancer science

Jin H,Liu P,Wu Y,Meng X,Wu M,Han J,Tan X

Sat Sep 01 00:00:00 EDT 2018

Exosomal zinc transporter ZIP4 promotes cancer growth and is a novel diagnostic biomarker for pancreatic cancer.

Cancer science

Jin H,Liu P,Wu Y,Meng X,Wu M,Han J,Tan X

Sat Sep 01 00:00:00 EDT 2018

Fenugreek galactomannan and citrus pectin improve several parameters associated with glucose metabolism and modulate gut microbiota in mice.

Nutrition (Burbank, Los Angeles County, Calif.)

Shtriker MG,Hahn M,Taieb E,Nyska A,Moallem U,Tirosh O,Madar Z

Thu Feb 01 00:00:00 EST 2018

Fenugreek galactomannan and citrus pectin improve several parameters associated with glucose metabolism and modulate gut microbiota in mice.

Nutrition (Burbank, Los Angeles County, Calif.)

Shtriker MG,Hahn M,Taieb E,Nyska A,Moallem U,Tirosh O,Madar Z

Thu Feb 01 00:00:00 EST 2018

Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction.

Redox biology

Liu Y,Li LN,Guo S,Zhao XY,Liu YZ,Liang C,Tu S,Wang D,Li L,Dong JZ,Gao L,Yang HB

Sat Sep 01 00:00:00 EDT 2018

Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction.

Redox biology

Liu Y,Li LN,Guo S,Zhao XY,Liu YZ,Liang C,Tu S,Wang D,Li L,Dong JZ,Gao L,Yang HB

Sat Sep 01 00:00:00 EDT 2018

Activation of adiponectin receptors has negative impact on muscle mass in C2C12 myotubes and fast-type mouse skeletal muscle.

PloS one

Ito R,Higa M,Goto A,Aoshima M,Ikuta A,Ohashi K,Yokoyama S,Ohno Y,Egawa T,Miyata H,Goto K

Mon Mar 25 00:00:00 EDT 2019

Activation of adiponectin receptors has negative impact on muscle mass in C2C12 myotubes and fast-type mouse skeletal muscle.

PloS one

Ito R,Higa M,Goto A,Aoshima M,Ikuta A,Ohashi K,Yokoyama S,Ohno Y,Egawa T,Miyata H,Goto K

Mon Mar 25 00:00:00 EDT 2019

Butyrate alleviates high fat diet-induced obesity through activation of adiponectin-mediated pathway and stimulation of mitochondrial function in the skeletal muscle of mice.

Oncotarget

Hong J,Jia Y,Pan S,Jia L,Li H,Han Z,Cai D,Zhao R

Tue Aug 30 00:00:00 EDT 2016

Effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD.

Annals of hepatology

Ma H,You GP,Cui F,Chen LF,Yang XJ,Chen LG,Lu HD,Zhang WQ

Mon Aug 24 00:00:00 EDT 2015

Effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD.

Annals of hepatology

Ma H,You GP,Cui F,Chen LF,Yang XJ,Chen LG,Lu HD,Zhang WQ

Mon Aug 24 00:00:00 EDT 2015

Effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD.

Annals of hepatology

Ma H,You GP,Cui F,Chen LF,Yang XJ,Chen LG,Lu HD,Zhang WQ

Mon Aug 24 00:00:00 EDT 2015

Effects of a low-fat diet on the hepatic expression of adiponectin and its receptors in rats with NAFLD.

Annals of hepatology

Ma H,You GP,Cui F,Chen LF,Yang XJ,Chen LG,Lu HD,Zhang WQ

Mon Aug 24 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Adiponectin receptor protein 1; Adiponectin receptor protein 2; Progestin and adipoQ receptor family member 1; Progestin and adipoQ receptor family member 2; Progestin and adipoQ receptor family member I; Progestin and adipoQ receptor family member II

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Gene Aliases: 1110001I14Rik; 2810031L11Rik; ACDCR1; ACDCR2; ADCR2; ADIPOR1; ADIPOR2; AI115388; AW554121; CGI-45; CGI45; D6Ucla1e; PAQR1; PAQR2; Parq1; Parq2; TESBP1A

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UniProt ID: (Human) Q96A54, (Human) Q86V24, (Mouse) Q91VH1, (Mouse) Q8BQS5

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Entrez Gene ID: (Human) 51094, (Human) 79602, (Rat) 289036, (Mouse) 72674, (Rat) 312670, (Mouse) 68465

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Function(s)

protein kinase binding identical protein binding metal ion binding protein heterodimerization activity adiponectin binding adipokinetic hormone receptor activity receptor activity hormone binding G-protein coupled receptor

Process(es)

G-protein coupled receptor signaling pathway hormone-mediated signaling pathway negative regulation of epithelial cell migration negative regulation of epithelial to mesenchymal transition regulation of glucose metabolic process regulation of lipid metabolic process fatty acid oxidation negative regulation of cell growth leptin-mediated signaling pathway adiponectin-activated signaling pathway glucose homeostasis negative regulation of JAK-STAT cascade positive regulation of JAK-STAT cascade positive regulation of insulin receptor signaling pathway negative regulation of NIK/NF-kappaB signaling heart development female pregnancy response to nutrient positive regulation of glucose import vascular wound healing lipid metabolic process fatty acid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Adiponectin receptor Polyclonal Antibody

Adiponectin receptor Antibody (14361-1-AP) in WB

Product Details

14361-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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