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Aldh1l1 Antibody (600-101-HB6) in ICC/IF
Immunofluorescence Microscopy of Goat anti-Aldh1l1 antibody. Cells: HepG2 cells. Fixation: 100% MeOH. Permeabilization: 0.3%Triton X-100. Primary antibody: Anti-Aldh1l1 antibody at 15µg/mL overnight at 2-8°C. Secondary antibody: Donkey Anti-Goat IgG DyLight™ 488 Conjugated Preadsorbed (p/n 605-741-125) at 5µg/mL for 1 h at RT. Localization: cytoplasm. Images were deconvoluted. Staining: (A) DAPI. (B) Anti-Adlh1l1 antibody+secondary. (C) Merged A+B. (D) Secondary Only.
Product Details
600-101-HB6
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Aldh1l1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Aldh1l1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.5M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Aldh1l1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and rat.
Target Information
ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10-formyltetrahydrofolate dehydrogenase; 10-FTHFDH; 10fTHF; 10FTHFDH; Aldehyde dehydrogenase family 1 member L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; formyltetrahydrofolate dehydrogenase
Gene Aliases: 1810048F20Rik; Aldh1l1; FDH; Fthfd; Neut2
UniProt ID: (Mouse) Q8R0Y6
Entrez Gene ID: (Mouse) 107747
catalytic activity
aldehyde dehydrogenase (NAD) activity
aldehyde dehydrogenase [NAD(P)+] activity
protein binding
methyltransferase activity
formyltetrahydrofolate dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
hydroxymethyl-, formyl- and related transferase activity
protein complex binding
aldehyde dehydrogenase (NADP+) activity
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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