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Invitrogen
Alexa Fluor 488 Recombinant Rabbit Monoclonal Antibody (15H9L93)
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Alexa Fluor 488 Antibody (704060) in ICC/IF
Immunofluorescent analysis of Alexa Fluor™ 488 was performed using 70% confluent HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with H3 Recombinant Rabbit Monoclonal Antibody (Product # 711055, 1:500) at 4 degree celsius overnight and then labeled with... View More
Product Details
704060
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
15H9L93
Immunogen
Alexa Fluor® 488 dye conjugated to KLH
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3090464
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes. The Alexa Fluor® 488 dye possesses near identical spectral properties and quantum yield to fluorescein isothiocyanate (FITC), but produces brighter, more photostable conjugates. These conjugates are used for imaging and other applications requiring increased sensitivity without being overly impacted by environmental factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AF488; Alexa 489; AlexaFluor488
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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