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FIGURE: 1 / 4
Alpha-2-macroglobulin Antibody (M1510-23) in ICC/IF
ICC staining Alpha-2-macroglobulin in H22 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Alpha-2-macroglobulin monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS. Alexa Fluor™ 488 Goat anti-Mouse Ig... View More
Product Details
M1510-23
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
8-B10
Immunogen
Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Target Information
Alpha 2-Macroglobulin (a2M), is a approximately 720-kDa homotetrameric glycoprotein composed of four identical approximately 180 kDa subunits. aAlpha 2M shares with other alpha-macroglobulins, like the complement components C3 and C4 and the pregnancy zone protein PZP, an extraordinary binding capacity for a variety of ligands. This allows the alpha-macroglobulins to serve as humoral defense barriers against foreign peptides in the plasma. Alpha 2M interacts and captures virtually any proteinase, often referred to as a panprotease inhibitor. In the brain of Alzheimer's disease (AD) patients, Alpha 2M also has been localized to diffuse amyloid plaques, supporting an important role for alpha 2M in AD etiopathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A2mp; Alpha 2 M; Alpha-2-M; Alpha-2-M (A2M); Alpha-2-macroglobulin; Alpha-2-macroglobulin-P; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; DKFZp779B086; S863 7
Gene Aliases: A2M; A2m1; A2maa; A2MAC1; A2MD; A2mp; CPAMD5; FWP007; Mam; S863-7
UniProt ID: (Human) P01023, (Mouse) Q6GQT1, (Rat) P06238
Entrez Gene ID: (Human) 2, (Mouse) 232345, (Rat) 24153
protease binding
serine-type endopeptidase inhibitor activity
GTPase activator activity
receptor binding
protein binding
growth factor binding
enzyme binding
interleukin-8 binding
interleukin-1 binding
tumor necrosis factor binding
calcium-dependent protein binding
endopeptidase inhibitor activity
peptidase inhibitor activity
protease inhibitor
negative regulation of complement activation, lectin pathway
platelet degranulation
blood coagulation, intrinsic pathway
negative regulation of endopeptidase activity
extracellular matrix disassembly
positive regulation of GTPase activity
stem cell differentiation
regulation of small GTPase mediated signal transduction
female pregnancy
negative regulation of peptidase activity
acute-phase response
inflammatory response
response to nutrient
response to wounding
response to carbon dioxide
response to glucocorticoid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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