Invitrogen

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 570, eBioscience™

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Alpha-Smooth Muscle Actin Antibody (41-9760-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma tissue using 1 µg/mL Mouse IgG2a, K Isotype Control eFluor® 570 (left) or 1 µg/mL Anti-Alpha-Smooth Muscle Actin eFluor® 570 (right). Nuclei are stained with DAPI.

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)

Product Image Gallery

Product Details

41-9760-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:100

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 9 publications 9 publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1A4

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573631

Product Specific Information

Description: The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin. Alpha-smooth muscle actin is a 42 kDa protein and is a major component of the cytoskeletal structural network. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in the parenchyma and stroma of various tissues. Alpha-smooth muscle actin is useful in the identification of leiomyomas, leiomycosarcomas and pleomorphic adenomas and is used as a prognostic marker for basal cell carcinoma.

Applications Reported: This 1A4 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 1A4 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pericyte stem cells induce Ly6G⁺ cell accumulation and immunotherapy resistance in pancreatic cancer.

EMBO reports

Wu Z,Thierry K,Bachy S,Zhang X,Gamradt P,Hernandez-Vargas H,Mikaelian I,Tonon L,Pommier R,Zhao Y,Bertolino P,Hennino A

41-9760-82 was used in Immunohistochemistry-immunofluorescence to reveal the existence of a cell population that instructs immunosuppressive myeloid cell responses to bypass PD-1 targeting and thus suggest potential new approaches for overcoming resistance to immunotherapy in clinical settings.

Wed Apr 05 00:00:00 EDT 2023

Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.

Communications biology

Rodriguez E,Boelaars K,Brown K,Madunić K,van Ee T,Dijk F,Verheij J,Li RJE,Schetters STT,Meijer LL,Le Large TYS,Driehuis E,Clevers H,Bruijns SCM,O'Toole T,van Vliet SJ,Bijlsma MF,Wuhrer M,Kazemier G,Giovannetti E,Garcia-Vallejo JJ,van Kooyk Y

41-9760-82 was used in Flow cytometry/Cell sorting to show mechanisms by which the glyco-code contributes to the tolerogenic microenvironment in PDAC.

Tue Jan 11 00:00:00 EST 2022

Gut-liver axis modulation of Panax notoginseng saponins in nonalcoholic fatty liver disease.

Hepatology international

Xu Y,Wang N,Tan HY,Li S,Zhang C,Feng Y

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.

Frontiers in pharmacology

El-Sherbiny M,Atef H,Eladl MA,Mohamed AS,El-Shafey M,Ali HS,Zaitone SA,Alomar SY,Alqahtani SAM,Aloyouni SY,Attia MA

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Tue May 11 00:00:00 EDT 2021

Chrysin Ameliorates Cyclosporine-A-Induced Renal Fibrosis by Inhibiting TGF-β₁-Induced Epithelial-Mesenchymal Transition.

International journal of molecular sciences

Nagavally RR,Sunilkumar S,Akhtar M,Trombetta LD,Ford SM

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Western Blot

Thu Sep 23 00:00:00 EDT 2021

An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.

Communications biology

Gilmore AC,Flaherty SJ,Somasundaram V,Scheiblin DA,Lockett SJ,Wink DA,Heinz WF

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Thu Apr 15 00:00:00 EDT 2021

An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.

Communications biology

Gilmore AC,Flaherty SJ,Somasundaram V,Scheiblin DA,Lockett SJ,Wink DA,Heinz WF

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Thu Apr 15 00:00:00 EDT 2021

The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation.

EBioMedicine

Panera N,Meroni M,Longo M,Crudele A,Valenti L,Bellacchio E,Miele L,D'Oria V,Paolini E,Maggioni M,Fracanzani AL,Alisi A,Dongiovanni P

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Mon Mar 01 00:00:00 EST 2021

Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.

Journal of thrombosis and haemostasis : JTH

Suess PM,Smith SA,Morrissey JH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Sun Nov 01 00:00:00 EDT 2020

Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype.

Frontiers in cardiovascular medicine

Gonzalez BA,Perez-Nevarez M,Mirza A,Perez MG,Lin YM,Hsu CD,Caobi A,Raymond A,Gomez Hernandez ME,Fernandez-Lima F,George F,Ramaswamy S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus.

Cancer biology & therapy

Wang T,Xie S,Luo R,Shi L,Bai P,Wang X,Wan R,Deng J,Wu Z,Li W,Xiao W,Wang Y,Chen B,Zhang K,Xing J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Mon Mar 29 00:00:00 EDT 2021

Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene-Deficient Mouse Model of Pancreatic Cancer.

Translational oncology

Yazawa K,Nakamura F,Masukawa D,Sato S,Hiroshima Y,Yabushita Y,Mori R,Matsuyama R,Kato I,Taniguchi H,Goshima Y,Endo I

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2020

miR-135a Alleviates Silica-Induced Pulmonary Fibrosis by Targeting NF-κB/Inflammatory Signaling Pathway.

Mediators of inflammation

Xie B,Lu C,Chen C,Zhou J,Deng Z

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Mon Jun 07 00:00:00 EDT 2021

A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula.

Journal of vascular research

Somarathna M,Isayeva-Waldrop T,Al-Balas A,Guo L,Lee T

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Mon Sep 14 00:00:00 EDT 2020

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro.

International journal of cell biology

Polley A,Khanam R,Sengupta A,Chakraborty S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Maintaining resting cardiac fibroblasts in vitro by disrupting mechanotransduction.

PloS one

Gilles G,McCulloch AD,Brakebusch CH,Herum KM

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Mon Dec 21 00:00:00 EST 2020

The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity.

Scientific reports

Gorchs L,Ahmed S,Mayer C,Knauf A,Fernández Moro C,Svensson M,Heuchel R,Rangelova E,Bergman P,Kaipe H

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Thu Oct 15 00:00:00 EDT 2020

Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth.

Cell reports

Davidson S,Efremova M,Riedel A,Mahata B,Pramanik J,Huuhtanen J,Kar G,Vento-Tormo R,Hagai T,Chen X,Haniffa MA,Shields JD,Teichmann SA

41-9760-82 was used in Flow Cytometry to identify complex interplays and increase stromal diversity as a tumor develops, revealing that stromal cells acquire the capacity to modulate immune landscapes from early disease.

Tue May 19 00:00:00 EDT 2020

Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth.

Cell reports

Davidson S,Efremova M,Riedel A,Mahata B,Pramanik J,Huuhtanen J,Kar G,Vento-Tormo R,Hagai T,Chen X,Haniffa MA,Shields JD,Teichmann SA

Tue May 19 00:00:00 EDT 2020

A mouse model of stenosis distal to an arteriovenous fistula recapitulates human central venous stenosis.

JVS-vascular science

Taniguchi R,Ono S,Isaji T,Gorecka J,Lee SR,Matsubara Y,Yatsula B,Koizumi J,Nishibe T,Hoshina K,Dardik A

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Wed Apr 20 00:00:00 EDT 2022

Material-Induced Venosome-Supported Bone Tubes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Charbonnier B,Baradaran A,Sato D,Alghamdi O,Zhang Z,Zhang YL,Gbureck U,Gilardino M,Harvey E,Makhoul N,Barralet J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Wed Sep 04 00:00:00 EDT 2019

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

Stem cell research & therapy

Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Western Blot

Fri Aug 23 00:00:00 EDT 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart.

The Journal of clinical investigation

Fu X,Khalil H,Kanisicak O,Boyer JG,Vagnozzi RJ,Maliken BD,Sargent MA,Prasad V,Valiente-Alandi I,Blaxall BC,Molkentin JD

41-9760 was used in Flow cytometry/Cell sorting to analyse and classify resident cardiac fibroblast dynamics during myocardial infarction injury and stable scar formation.

Tue May 01 00:00:00 EDT 2018

Human ISL1⁺ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction.

Molecular therapy : the journal of the American Society of Gene Therapy

Foo KS,Lehtinen ML,Leung CY,Lian X,Xu J,Keung W,Geng L,Kolstad TRS,Thams S,Wong AO,Wong N,Bylund K,Zhou C,He X,Jin SB,Clarke J,Lendahl U,Li RA,Louch WE,Chien KR

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunohistochemistry

Thu Jul 05 00:00:00 EDT 2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 41-9760-82) in Immunofluorescence

Fri Aug 12 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

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UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

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Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

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Function(s)

ATP binding protein kinase binding nucleotide binding protein binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

muscle contraction regulation of blood pressure response to virus positive regulation of gene expression vascular smooth muscle contraction glomerular mesangial cell development mesenchyme migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 570, eBioscience™

Alpha-Smooth Muscle Actin Antibody (41-9760-82) in IHC (P)

Product Details

41-9760-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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