Invitrogen

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

30 Published Figures

34 References

View all (72) Alpha-Smooth Muscle Actin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 46

Alpha-Smooth Muscle Actin Antibody (50-9760-82) in ICC/IF

Immunofluorescence analysis of SMA was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton for 15 minutes and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience™ (Product # 50-9760-82) at 5 µg/mL in 0.1% B... View More

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (IHC)

Alpha-Smooth Muscle Actin Antibody in Western Blot (WB)

Alpha-Smooth Muscle Actin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Alpha-Smooth Muscle Actin Antibody in Immunohistochemistry (Frozen) (IHC (F))

Alpha-Smooth Muscle Actin Antibody in Flow Cytometry (Flow)

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Product Details

50-9760-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

1:100

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 8 publications 8 publications

Flow Cytometry (Flow)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A4

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574362

Product Specific Information

Description: The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin. Alpha-smooth muscle actin is a 42 kDa protein and is a major component of the cytoskeletal structural network. This 1A4 antibody is specific for the alpha form of muscle actin only, which is expressed by smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells. It is also expressed in the parenchyma and stroma of various tissues. Alpha-smooth muscle actin is useful in the identification of leiomyomas, leiomycosarcomas and pleomorphic adenomas and is used as a prognostic marker for basal cell carcinoma.

Applications Reported: This 1A4 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This 1A4 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 1A4 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glymphatic System and Subsidiary Pathways Drive Nanoparticles Away from the Brain.

Research (Washington, D.C.)

Liu R,Jia W,Wang Y,Hu C,Yu W,Huang Y,Wang L,Gao H

50-9760-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to reveal that microglia promotes glymphatic drainage of nanoclusters and passage across the BBB, providing a basis for further brain-delivery DDS.

Sat Apr 02 00:00:00 EDT 2022

Single-cell transcriptomics reveals functionally specialized vascular endothelium in brain.

eLife

Jeong HW,Diéguez-Hurtado R,Arf H,Song J,Park H,Kruse K,Sorokin L,Adams RH

50-9760-82 was used in Immunohistochemistry to provide novel insights into the heterogeneity of the cerebral vasculature and a useful resource for the molecular alterations associated with neuroinflammation and ageing.

Wed Oct 05 00:00:00 EDT 2022

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity.

Nature immunology

Prados A,Onder L,Cheng HW,Mörbe U,Lütge M,Gil-Cruz C,Perez-Shibayama C,Koliaraki V,Ludewig B,Kollias G

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Gut-liver axis modulation of Panax notoginseng saponins in nonalcoholic fatty liver disease.

Hepatology international

Xu Y,Wang N,Tan HY,Li S,Zhang C,Feng Y

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

IFNβ1 secreted by breast cancer cells undergoing chemotherapy reprograms stromal fibroblasts to support tumour growth after treatment.

Molecular oncology

Maia A,Gu Z,Koch A,Berdiel-Acer M,Will R,Schlesner M,Wiemann S

50-9760-82 was used in Flow cytometry/Cell sorting to investigate the impact of the tumour microenvironment on the fate of cancer cells after chemotherapy.

Sat May 01 00:00:00 EDT 2021

Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.

Frontiers in pharmacology

El-Sherbiny M,Atef H,Eladl MA,Mohamed AS,El-Shafey M,Ali HS,Zaitone SA,Alomar SY,Alqahtani SAM,Aloyouni SY,Attia MA

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Tue May 18 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy.

Cell death & disease

Zhang L,He J,Wang J,Liu J,Chen Z,Deng B,Wei L,Wu H,Liang B,Li H,Huang Y,Lu L,Yang Z,Xian S,Wang L

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Tue May 11 00:00:00 EDT 2021

Chrysin Ameliorates Cyclosporine-A-Induced Renal Fibrosis by Inhibiting TGF-β₁-Induced Epithelial-Mesenchymal Transition.

International journal of molecular sciences

Nagavally RR,Sunilkumar S,Akhtar M,Trombetta LD,Ford SM

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Western Blot

Thu Sep 23 00:00:00 EDT 2021

The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation.

EBioMedicine

Panera N,Meroni M,Longo M,Crudele A,Valenti L,Bellacchio E,Miele L,D'Oria V,Paolini E,Maggioni M,Fracanzani AL,Alisi A,Dongiovanni P

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Mar 01 00:00:00 EST 2021

Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice.

Scientific reports

Wilm TP,Tanton H,Mutter F,Foisor V,Middlehurst B,Ward K,Benameur T,Hastie N,Wilm B

50-9760-82 was used in Flow Cytometry to determine the spatio-temporal contribution of Wt1-expressing cells to the adult and postnatal intestine and peritoneal body wall, including the mesentery.

Thu Aug 05 00:00:00 EDT 2021

Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential.

Cell

Huang S,Ziegler CGK,Austin J,Mannoun N,Vukovic M,Ordovas-Montanes J,Shalek AK,von Andrian UH

50-9760-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to identify and functionally tested a sensory neuro-immune circuit that is responsive to lymph-borne inflammatory signals.

Thu Jan 21 00:00:00 EST 2021

Identification of a population of peripheral sensory neurons that regulates blood pressure.

Cell reports

Morelli C,Castaldi L,Brown SJ,Streich LL,Websdale A,Taberner FJ,Cerreti B,Barenghi A,Blum KM,Sawitzke J,Frank T,Steffens LK,Doleschall B,Serrao J,Ferrarini D,Lechner SG,Prevedel R,Heppenstall PA

50-9760-82 was used in Immunohistochemistry to describe a population of peripheral neurons marked by TrkC and Th that have cell bodies in the spinal ganglia and project to distal blood vessels.

Tue Jun 01 00:00:00 EDT 2021

An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients.

Communications biology

Gilmore AC,Flaherty SJ,Somasundaram V,Scheiblin DA,Lockett SJ,Wink DA,Heinz WF

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Thu Apr 15 00:00:00 EDT 2021

Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation.

Journal of thrombosis and haemostasis : JTH

Suess PM,Smith SA,Morrissey JH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Sun Nov 01 00:00:00 EDT 2020

Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype.

Frontiers in cardiovascular medicine

Gonzalez BA,Perez-Nevarez M,Mirza A,Perez MG,Lin YM,Hsu CD,Caobi A,Raymond A,Gomez Hernandez ME,Fernandez-Lima F,George F,Ramaswamy S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus.

Cancer biology & therapy

Wang T,Xie S,Luo R,Shi L,Bai P,Wang X,Wan R,Deng J,Wu Z,Li W,Xiao W,Wang Y,Chen B,Zhang K,Xing J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Mon Mar 29 00:00:00 EDT 2021

Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene-Deficient Mouse Model of Pancreatic Cancer.

Translational oncology

Yazawa K,Nakamura F,Masukawa D,Sato S,Hiroshima Y,Yabushita Y,Mori R,Matsuyama R,Kato I,Taniguchi H,Goshima Y,Endo I

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Sun Mar 01 00:00:00 EST 2020

miR-135a Alleviates Silica-Induced Pulmonary Fibrosis by Targeting NF-κB/Inflammatory Signaling Pathway.

Mediators of inflammation

Xie B,Lu C,Chen C,Zhou J,Deng Z

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Mon Jun 07 00:00:00 EDT 2021

A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula.

Journal of vascular research

Somarathna M,Isayeva-Waldrop T,Al-Balas A,Guo L,Lee T

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Mon Sep 14 00:00:00 EDT 2020

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro.

International journal of cell biology

Polley A,Khanam R,Sengupta A,Chakraborty S

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

50-9760 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms regulating the heterogeneity and behavior of resident vascular adventitial SCA1+ progenitor (AdvSca1) cells in homeostasis and injury responses.

Thu Dec 03 00:00:00 EST 2020

Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis.

JCI insight

Lu S,Jolly AJ,Strand KA,Dubner AM,Mutryn MF,Moulton KS,Nemenoff RA,Majesky MW,Weiser-Evans MC

Thu Dec 03 00:00:00 EST 2020

Maintaining resting cardiac fibroblasts in vitro by disrupting mechanotransduction.

PloS one

Gilles G,McCulloch AD,Brakebusch CH,Herum KM

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Mon Dec 21 00:00:00 EST 2020

The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity.

Scientific reports

Gorchs L,Ahmed S,Mayer C,Knauf A,Fernández Moro C,Svensson M,Heuchel R,Rangelova E,Bergman P,Kaipe H

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Thu Oct 15 00:00:00 EDT 2020

A mouse model of stenosis distal to an arteriovenous fistula recapitulates human central venous stenosis.

JVS-vascular science

Taniguchi R,Ono S,Isaji T,Gorecka J,Lee SR,Matsubara Y,Yatsula B,Koizumi J,Nishibe T,Hoshina K,Dardik A

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Wed Apr 20 00:00:00 EDT 2022

Material-Induced Venosome-Supported Bone Tubes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Charbonnier B,Baradaran A,Sato D,Alghamdi O,Zhang Z,Zhang YL,Gbureck U,Gilardino M,Harvey E,Makhoul N,Barralet J

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Wed Sep 04 00:00:00 EDT 2019

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

Stem cell research & therapy

Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Western Blot

Fri Aug 23 00:00:00 EDT 2019

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer.

Frontiers in immunology

Martinez LM,Robila V,Clark NM,Du W,Idowu MO,Rutkowski MR,Bos PD

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunocytochemistry

Tue Sep 29 00:00:00 EDT 2020

Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.

Nature communications

Diéguez-Hurtado R,Kato K,Giaimo BD,Nieminen-Kelhä M,Arf H,Ferrante F,Bartkuhn M,Zimmermann T,Bixel MG,Eilken HM,Adams S,Borggrefe T,Vajkoczy P,Adams RH

50-9760-82 was used in Immunohistochemistry to propose that brain pericytes can acquire deleterious properties that actively enhance vascular lesion formation and promote pathogenic processes.

Thu Jun 27 00:00:00 EDT 2019

Human ISL1⁺ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction.

Molecular therapy : the journal of the American Society of Gene Therapy

Foo KS,Lehtinen ML,Leung CY,Lian X,Xu J,Keung W,Geng L,Kolstad TRS,Thams S,Wong AO,Wong N,Bylund K,Zhou C,He X,Jin SB,Clarke J,Lendahl U,Li RA,Louch WE,Chien KR

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunohistochemistry

Thu Jul 05 00:00:00 EDT 2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system.

Nature communications

Kaur H,Carvalho J,Looso M,Singh P,Chennupati R,Preussner J,Günther S,Albarrán-Juárez J,Tischner D,Classen S,Offermanns S,Wettschureck N

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Flow Cytometry

Fri Jun 16 00:00:00 EDT 2017

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using Alpha-Smooth Muscle Actin monoclonal antibody (Product # 50-9760-82) in Immunofluorescence

Fri Aug 12 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AAT6; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; MYMY5; SMalphaA

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UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737

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Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475

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Function(s)

ATP binding protein kinase binding nucleotide binding protein binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

muscle contraction regulation of blood pressure response to virus positive regulation of gene expression vascular smooth muscle contraction glomerular mesangial cell development mesenchyme migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience™

Alpha-Smooth Muscle Actin Antibody (50-9760-82) in ICC/IF

Product Details

50-9760-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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