Proteintech

Amphiregulin Polyclonal Antibody

Amphiregulin Antibody in Immunocytochemistry (ICC/IF)

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Amphiregulin Antibody (16036-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using AREG antibody 16036-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Amphiregulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Amphiregulin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Amphiregulin Antibody in Western Blot (WB)

Amphiregulin Antibody in Flow Cytometry (Flow)

Amphiregulin Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16036-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Amphiregulin Fusion Protein Ag8907 (25-252 aa encoded by BC009799)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AGLDLNDTY SGKREPFSGD HSADGFEVTS RSEMSSGSEI SPVSEMPSSS EPSSGADYDY SEEYDNEPQI PGYIVDDSVR VEQVVKPPQN KTESENTSDK PKRKKKGGKN GKNRRNRKKK NPCNAEFQNF CIHGECKYIE HLEAVTCKCQ QEYFGERCGE KSMKTHSMID SSLSKIALAA IAAFMSAVIL TAVAVITVQL RRQYVRKYEG EAEERKKLRQ ENGNVHAIA (25-252 aa encoded by BC009799)

Target Information

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amphiregulin blockade decreases the levodopa-induced dyskinesia in a 6-hydroxydopamine Parkinson's disease mouse model.

CNS neuroscience & therapeutics

Kambey PA,Liu WY,Wu J,Tang C,Buberwa W,Saro A,Nyalali AMK,Gao D

Sun Oct 01 00:00:00 EDT 2023

Amphiregulin blockade decreases the levodopa-induced dyskinesia in a 6-hydroxydopamine Parkinson's disease mouse model.

CNS neuroscience & therapeutics

Kambey PA,Liu WY,Wu J,Tang C,Buberwa W,Saro A,Nyalali AMK,Gao D

Sun Oct 01 00:00:00 EDT 2023

Quercetin inhibits the amphiregulin/EGFR signaling-mediated renal tubular epithelial-mesenchymal transition and renal fibrosis in obstructive nephropathy.

Phytotherapy research : PTR

Wang Q,Wang F,Li X,Ma Z,Jiang D

Sun Jan 01 00:00:00 EST 2023

Quercetin inhibits the amphiregulin/EGFR signaling-mediated renal tubular epithelial-mesenchymal transition and renal fibrosis in obstructive nephropathy.

Phytotherapy research : PTR

Wang Q,Wang F,Li X,Ma Z,Jiang D

Sun Jan 01 00:00:00 EST 2023

Quercetin inhibits the amphiregulin/EGFR signaling-mediated renal tubular epithelial-mesenchymal transition and renal fibrosis in obstructive nephropathy.

Phytotherapy research : PTR

Wang Q,Wang F,Li X,Ma Z,Jiang D

Sun Jan 01 00:00:00 EST 2023

Quercetin inhibits the amphiregulin/EGFR signaling-mediated renal tubular epithelial-mesenchymal transition and renal fibrosis in obstructive nephropathy.

Phytotherapy research : PTR

Wang Q,Wang F,Li X,Ma Z,Jiang D

Sun Jan 01 00:00:00 EST 2023

Dapagliflozin delays renal fibrosis in diabetic kidney disease by inhibiting YAP/TAZ activation.

Life sciences

Feng L,Chen Y,Li N,Yang X,Zhou L,Li H,Wang T,Xie M,Liu H

Thu Jun 01 00:00:00 EDT 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 EST 2023

Adaptive transcriptomic and immune infiltrate responses in the tumor immune microenvironment following neoadjuvant chemotherapy in high grade serous ovarian cancer reveal novel prognostic associations and activation of pro-tumorigenic pathways.

Frontiers in immunology

James NE,Woodman M,De La Cruz P,Eurich K,Ozsoy MA,Schorl C,Hanley LC,Ribeiro JR

Fri Sep 23 00:00:00 EDT 2022

Establishment of a patient-derived mucoepidermoid carcinoma cell line with the CRTC1-MAML2 fusion gene.

Molecular and clinical oncology

Noguchi K,Kanda S,Yoshida K,Funaoka Y,Yamanegi K,Yoshikawa K,Takaoka K,Kishimoto H,Nakano Y

Tue Mar 01 00:00:00 EST 2022

Luteinizing hormone stimulates the expression of amphiregulin in human theca cells.

Journal of ovarian research

Liu Y,Zhong Y,Shen X,Guo X,Wu R,Yang T,Chen M

Wed Dec 07 00:00:00 EST 2022

Luteinizing hormone stimulates the expression of amphiregulin in human theca cells.

Journal of ovarian research

Liu Y,Zhong Y,Shen X,Guo X,Wu R,Yang T,Chen M

Wed Dec 07 00:00:00 EST 2022

Luteinizing hormone stimulates the expression of amphiregulin in human theca cells.

Journal of ovarian research

Liu Y,Zhong Y,Shen X,Guo X,Wu R,Yang T,Chen M

Wed Dec 07 00:00:00 EST 2022

AXL cooperates with EGFR to mediate neutrophil elastase-induced migration of prostate cancer cells.

iScience

Xiao Z,Hammes SR

Fri Nov 19 00:00:00 EST 2021

Extracellular Matrix-Bound FGF2 Mediates Estrogen Receptor Signaling and Therapeutic Response in Breast Cancer.

Molecular cancer research : MCR

DiGiacomo JW,Godet I,Trautmann-Rodriguez M,Gilkes DM

Fri Jan 01 00:00:00 EST 2021

IFI16 promotes cervical cancer progression by upregulating PD-L1 in immunomicroenvironment through STING-TBK1-NF-kB pathway.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Cai H,Yan L,Liu N,Xu M,Cai H

Sun Mar 01 00:00:00 EST 2020

AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Kubli SP,Bassi C,Roux C,Wakeham A,Göbl C,Zhou W,Jafari SM,Snow B,Jones L,Palomero L,Thu KL,Cassetta L,Soong D,Berger T,Ramachandran P,Baniasadi SP,Duncan G,Lindzen M,Yarden Y,Herranz C,Lazaro C,Chu MF,Haight J,Tinto P,Silvester J,Cescon DW,Petit A,Pettersson S,Pollard JW,Mak TW,Pujana MA,Cappello P,Gorrini C

Tue Feb 26 00:00:00 EST 2019

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Aging cell

Xu Q,Long Q,Zhu D,Fu D,Zhang B,Han L,Qian M,Guo J,Xu J,Cao L,Chin YE,Coppé JP,Lam EW,Campisi J,Sun Y

Sun Dec 01 00:00:00 EST 2019

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Aging cell

Xu Q,Long Q,Zhu D,Fu D,Zhang B,Han L,Qian M,Guo J,Xu J,Cao L,Chin YE,Coppé JP,Lam EW,Campisi J,Sun Y

Sun Dec 01 00:00:00 EST 2019

Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.

Aging cell

Xu Q,Long Q,Zhu D,Fu D,Zhang B,Han L,Qian M,Guo J,Xu J,Cao L,Chin YE,Coppé JP,Lam EW,Campisi J,Sun Y

Sun Dec 01 00:00:00 EST 2019

CBX2 Regulates Proliferation and Apoptosis via the Phosphorylation of YAP in Hepatocellular Carcinoma.

Journal of Cancer

Mao J,Tian Y,Wang C,Jiang K,Li R,Yao Y,Zhang R,Sun D,Liang R,Gao Z,Wang Q,Wang L

Thu Oct 01 00:00:00 EDT 2020

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Cancer research

Zhang G,Li X,Chen Q,Li J,Ruan Q,Chen YH,Yang X,Wan X

Wed May 01 00:00:00 EDT 2019

Loss of KIBRA function activates EGFR signaling by inducing AREG.

Oncotarget

Mussell AL,Denson KE,Shen H,Chen Y,Yang N,Frangou C,Zhang J

Fri Jul 06 00:00:00 EDT 2018

Loss of KIBRA function activates EGFR signaling by inducing AREG.

Oncotarget

Mussell AL,Denson KE,Shen H,Chen Y,Yang N,Frangou C,Zhang J

Fri Jul 06 00:00:00 EDT 2018

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Oncogene

Stoll SW,Stuart PE,Swindell WR,Tsoi LC,Li B,Gandarillas A,Lambert S,Johnston A,Nair RP,Elder JT

Thu Apr 21 00:00:00 EDT 2016

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Oncogene

Stoll SW,Stuart PE,Swindell WR,Tsoi LC,Li B,Gandarillas A,Lambert S,Johnston A,Nair RP,Elder JT

Thu Apr 21 00:00:00 EDT 2016

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Oncogene

Stoll SW,Stuart PE,Swindell WR,Tsoi LC,Li B,Gandarillas A,Lambert S,Johnston A,Nair RP,Elder JT

Thu Apr 21 00:00:00 EDT 2016

The EGF receptor ligand amphiregulin controls cell division via FoxM1.

Oncogene

Stoll SW,Stuart PE,Swindell WR,Tsoi LC,Li B,Gandarillas A,Lambert S,Johnston A,Nair RP,Elder JT

Thu Apr 21 00:00:00 EDT 2016

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

The Journal of investigative dermatology

Stoll SW,Stuart PE,Lambert S,Gandarillas A,Rittié L,Johnston A,Elder JT

Mon Feb 01 00:00:00 EST 2016

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

The Journal of investigative dermatology

Stoll SW,Stuart PE,Lambert S,Gandarillas A,Rittié L,Johnston A,Elder JT

Mon Feb 01 00:00:00 EST 2016

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

The Journal of investigative dermatology

Stoll SW,Stuart PE,Lambert S,Gandarillas A,Rittié L,Johnston A,Elder JT

Mon Feb 01 00:00:00 EST 2016

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.

The Journal of investigative dermatology

Stoll SW,Stuart PE,Lambert S,Gandarillas A,Rittié L,Johnston A,Elder JT

Mon Feb 01 00:00:00 EST 2016

TAZ induces growth factor-independent proliferation through activation of EGFR ligand amphiregulin.

Cell cycle (Georgetown, Tex.)

Yang N,Morrison CD,Liu P,Miecznikowski J,Bshara W,Han S,Zhu Q,Omilian AR,Li X,Zhang J

Wed Aug 01 00:00:00 EDT 2012

TAZ induces growth factor-independent proliferation through activation of EGFR ligand amphiregulin.

Cell cycle (Georgetown, Tex.)

Yang N,Morrison CD,Liu P,Miecznikowski J,Bshara W,Han S,Zhu Q,Omilian AR,Li X,Zhang J

Wed Aug 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor

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Gene Aliases: AR; AREG; AREGB; CRDGF; SDGF

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UniProt ID: (Human) P15514

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Entrez Gene ID: (Human) 374

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Function(s)

cytokine activity epidermal growth factor receptor binding protein binding growth factor activity growth factor

Process(es)

ER to Golgi vesicle-mediated transport epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell-cell signaling cell proliferation positive regulation of cell proliferation glial cell proliferation neuron projection development response to estradiol positive regulation of phosphorylation response to hydrogen peroxide response to peptide hormone negative regulation of osteoblast differentiation positive regulation of DNA replication COPII vesicle coating response to glucocorticoid response to cAMP dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis mammary gland branching involved in thelarche mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Amphiregulin Polyclonal Antibody

Amphiregulin Antibody (16036-1-AP) in ICC/IF

Product Details

16036-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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