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Amphiregulin Antibody (500-P322-1MG) in WB
Western Blot: To detect Human Amphiregulin by Western Blot analysis Amphiregulin Polyclonal Antibody (Product # 500-P322-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P322-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human Amphiregulin
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930404
Product Specific Information
AA Sequence of recombinant protein: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography employing an immobilized Human Amphiregulin matrix.
Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Amphiregulin (500-P322Bt) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human Amphiregulin.
Western Blot: To detect Human Amphiregulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor
Gene Aliases: AR; AREG; AREGB; CRDGF; SDGF
UniProt ID: (Human) P15514
Entrez Gene ID: (Human) 374
ER to Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
cell proliferation
positive regulation of cell proliferation
glial cell proliferation
neuron projection development
response to estradiol
positive regulation of phosphorylation
response to hydrogen peroxide
response to peptide hormone
negative regulation of osteoblast differentiation
positive regulation of DNA replication
COPII vesicle coating
response to glucocorticoid
response to cAMP
dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis
mammary gland branching involved in thelarche
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
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