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Invitrogen
Amphiregulin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Amphiregulin Antibody (PA5-102501) in IHC (P)
Immunohistochemistry analysis of Amphiregulin in human ovarian cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Amphiregulin polyclonal antibody (Product # PA5-102501) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-102501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AREG(Accession P15514), corresponding to amino acid residues G60-P110.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851903
Product Specific Information
Antibody detects endogenous levels of total AREG.
Target Information
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Amphiregulin; amphiregulin B; AR; Colorectum cell-derived growth factor; CRDGF; MGC13647; Schwannoma-derived growth factor; SDGF
Gene Aliases: AR; AREG; AREGB; CRDGF; Mcub; SDGF
UniProt ID: (Human) P15514, (Rat) P24338, (Mouse) P31955
Entrez Gene ID: (Human) 374, (Rat) 29183, (Mouse) 11839
ER to Golgi vesicle-mediated transport
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
cell proliferation
positive regulation of cell proliferation
glial cell proliferation
neuron projection development
response to estradiol
positive regulation of phosphorylation
response to hydrogen peroxide
response to peptide hormone
negative regulation of osteoblast differentiation
positive regulation of DNA replication
COPII vesicle coating
response to glucocorticoid
response to cAMP
dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis
mammary gland branching involved in thelarche
mammary gland alveolus development
epithelial cell proliferation involved in mammary gland duct elongation
central nervous system development
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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