Invitrogen

Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

11 References

View all (59) beta Amyloid antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Amyloid Precursor Protein Antibody (OMA1-03132) in ICC/IF

Immunofluorescence analysis of Amyloid Precursor Protein was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1) (Product # OMA1-03132) at 1:200 dilut... View More

Amyloid Precursor Protein Antibody in Western Blot (WB)

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Product Details

OMA1-03132

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

ELISA (ELISA)

10 µg/mL

View 6 publications 6 publications

Immunoprecipitation (IP)

25 µg/mL*

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

mAbP2-1

Immunogen

Native, secreted form of APP

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_325526

Product Specific Information

OMA1-03132 detects amyloid precursor protein (APP) from human and monkey tissues. This antibody is specific for native, non-denatured protein, and does not cross-react with mouse, rat APP or other APP homologs.

OMA1-03132 has been successfully used in Western blot (non-reducing conditions), immunocytochemistry, immunoprecipitation and ELISA procedures. This antibody does not work in in IHC on paraffin embedded tissues nor does it recognize APP if the samples are boiled in Laemmli (SDS) sample buffer containing a reducing agent such as DTT or ß-ME. Can be used in immunoprecipitation utilizing 25 µg/mL of OMA1-03132 linked sepharose.

OMA1-03132 antigen is the native, secreted form of human APP. The epitope recognized by OMA1-03132 maps to residues 104-118 of APP

Target Information

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.

Cell systems

Hark TJ,Rao NR,Castillon C,Basta T,Smukowski S,Bao H,Upadhyay A,Bomba-Warczak E,Nomura T,O'Toole ET,Morgan GP,Ali L,Saito T,Guillermier C,Saido TC,Steinhauser ML,Stowell MHB,Chapman ER,Contractor A,Savas JN

OMA1-03132 was used in Western Blotting to observe protein turnover at early stages of amyloid beta (Aβ) proteotoxicity.

Wed Feb 17 00:00:00 EST 2021

Aminomethyl-Derived Beta Secretase (BACE1) Inhibitors: Engaging Gly230 without an Anilide Functionality.

Journal of medicinal chemistry

Butler CR,Ogilvie K,Martinez-Alsina L,Barreiro G,Beck EM,Nolan CE,Atchison K,Benvenuti E,Buzon L,Doran S,Gonzales C,Helal CJ,Hou X,Hsu MH,Johnson EF,Lapham K,Lanyon L,Parris K,O'Neill BT,Riddell D,Robshaw A,Vajdos F,Brodney MA

OMA103132 was used in ELISA to describe an alternative aminomethyl linker for beta-secretase inhibitors that with potency similar to the current anilide moiety and improves brain penetration

Thu Jan 12 00:00:00 EST 2017

Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A.

Molecular neurodegeneration

Willén K,Edgar JR,Hasegawa T,Tanaka N,Futter CE,Gouras GK

Published figure using Amyloid Precursor Protein monoclonal antibody (Product # OMA1-03132) in Immunofluorescence

Wed Aug 23 00:00:00 EDT 2017

Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A.

Molecular neurodegeneration

Willén K,Edgar JR,Hasegawa T,Tanaka N,Futter CE,Gouras GK

Published figure using Amyloid Precursor Protein monoclonal antibody (Product # OMA1-03132) in Immunofluorescence

Wed Aug 23 00:00:00 EDT 2017

Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP.

Nature communications

Klementieva O,Willén K,Martinsson I,Israelsson B,Engdahl A,Cladera J,Uvdal P,Gouras GK

OMA103132 was used in western blot to characterize physiological conformations of Abeta and amyloid precursor protein

Mon Mar 13 00:00:00 EDT 2017

Chemoproteomic profiling reveals that cathepsin D off-target activity drives ocular toxicity of β-secretase inhibitors.

Nature communications

Zuhl AM,Nolan CE,Brodney MA,Niessen S,Atchison K,Houle C,Karanian DA,Ambroise C,Brulet JW,Beck EM,Doran SD,O'Neill BT,Am Ende CW,Chang C,Geoghegan KF,West GM,Judkins JC,Hou X,Riddell DR,Johnson DS

OMA103132 was used in ELISA to demonstrate that cathepsin D is an off-target of BACE1 inhibitors in human cells

Tue Oct 11 00:00:00 EDT 2016

Discovery of a series of efficient, centrally efficacious BACE1 inhibitors through structure-based drug design.

Journal of medicinal chemistry

Butler CR,Brodney MA,Beck EM,Barreiro G,Nolan CE,Pan F,Vajdos F,Parris K,Varghese AH,Helal CJ,Lira R,Doran SD,Riddell DR,Buzon LM,Dutra JK,Martinez-Alsina LA,Ogilvie K,Murray JC,Young JM,Atchison K,Robshaw A,Gonzales C,Wang J,Zhang Y,O'Neill BT

OMA1-03132 was used in ELISA to generate and characterize new BACE1 inhibitors for the treatment of Alzheimer's disease

Thu Mar 26 00:00:00 EDT 2015

Utilizing structures of CYP2D6 and BACE1 complexes to reduce risk of drug-drug interactions with a novel series of centrally efficacious BACE1 inhibitors.

Journal of medicinal chemistry

Brodney MA,Beck EM,Butler CR,Barreiro G,Johnson EF,Riddell D,Parris K,Nolan CE,Fan Y,Atchison K,Gonzales C,Robshaw AE,Doran SD,Bundesmann MW,Buzon L,Dutra J,Henegar K,LaChapelle E,Hou X,Rogers BN,Pandit J,Lira R,Martinez-Alsina L,Mikochik P,Murray JC,Ogilvie K,Price L,Sakya SM,Yu A,Zhang Y,O'Neill BT

OMA1-03132 was used in ELISA to generate and characterize new BACE1 inhibitors for the treatment of Alzheimer's disease

Thu Apr 09 00:00:00 EDT 2015

Spirocyclic sulfamides as β-secretase 1 (BACE-1) inhibitors for the treatment of Alzheimer's disease: utilization of structure based drug design, WaterMap, and CNS penetration studies to identify centrally efficacious inhibitors.

Journal of medicinal chemistry

Brodney MA,Barreiro G,Ogilvie K,Hajos-Korcsok E,Murray J,Vajdos F,Ambroise C,Christoffersen C,Fisher K,Lanyon L,Liu J,Nolan CE,Withka JM,Borzilleri KA,Efremov I,Oborski CE,Varghese A,O'Neill BT

OMA1-03132 was used in ELISA to report on the design of novel spirocyclic sulphamide BACE-1 inhibitors as potential Alzheimer's disease therapeutics

Thu Nov 08 00:00:00 EST 2012

Identification of novel N-terminal fragments of amyloid precursor protein in cerebrospinal fluid.

Experimental neurology

Portelius E,Brinkmalm G,Tran A,Andreasson U,Zetterberg H,Westman-Brinkmalm A,Blennow K,Ohrfelt A

OMA1-03132 was used in immunoprecipitation to search for N-terminal fragments of amyloid precursor protein in cerebrospinal fluid

Tue Jun 01 00:00:00 EDT 2010

X11alpha modulates secretory and endocytic trafficking and metabolism of amyloid precursor protein: mutational analysis of the YENPTY sequence.

Neuroscience

King GD,Perez RG,Steinhilb ML,Gaut JR,Turner RS

OMA1-03132 was used in ELISA to investigate the role of the -YENP- motif of the amyloid precursor protein.

Tue Sep 30 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: ABPP; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; Amyloid precursor protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CVAP; OTTHUMP00000096096; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; PreA4 751; Protease nexin-II; testicular tissue protein Li 2

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Gene Aliases: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

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UniProt ID: (Human) P05067

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Entrez Gene ID: (Human) 351

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Function(s)

DNA binding serine-type endopeptidase inhibitor activity receptor binding protein binding heparin binding peptidase activator activity enzyme binding identical protein binding transition metal ion binding PTB domain binding growth factor receptor binding protease inhibitor

Process(es)

response to yeast suckling behavior platelet degranulation mRNA polyadenylation regulation of translation protein phosphorylation cellular copper ion homeostasis endocytosis response to oxidative stress cell adhesion regulation of epidermal growth factor-activated receptor activity Notch signaling pathway axonogenesis mating behavior locomotory behavior axo-dendritic transport cholesterol metabolic process adult locomotory behavior visual learning cellular process response to lead ion negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle axon midline choice point recognition neuron remodeling dendrite development antibacterial humoral response antifungal humoral response extracellular matrix organization forebrain development neuron projection development ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth regulation of protein binding cellular protein metabolic process innate immune response negative regulation of neuron differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter collateral sprouting in absence of injury regulation of synapse structure or activity defense response to Gram-negative bacterium defense response to Gram-positive bacterium neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis cellular response to cAMP cellular response to norepinephrine stimulus amyloid fibril formation cellular response to nerve growth factor stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

Amyloid Precursor Protein Antibody (OMA1-03132) in ICC/IF

Product Details

OMA1-03132

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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