Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 6
Androgen Receptor Antibody (PA1-110) in IHC (P)
Immunohistochemistry analysis of Androgen Receptor showing staining in the cytoplasm and nucleus of paraffin-treated human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methan... View More
Product Details
PA1-110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Hamster,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein containing residues 331-572 of human androgen receptor.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_325809
Product Specific Information
PA1-110 detects androgen receptor (AR) from human, primate, rat, and mouse tissues. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-110 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from rat brain homogenate. Immunohistochemical staining of AR in rat brain with PA1-110 results in intense nuclear staining.
For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.
The PA1-110 immunogen is a fusion protein containing residues 331-572 of human androgen receptor.
Target Information
The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: androgen nuclear receptor variant 2; Androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; AR-A; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; Variant AR45
Gene Aliases: AIS; Andr; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
UniProt ID: (Human) P10275, (Rat) P15207, (Mouse) P19091
Entrez Gene ID: (Human) 367, (Rat) 24208, (Mouse) 11835
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
androgen receptor activity
receptor binding
steroid binding
androgen binding
protein binding
beta-catenin binding
transcription factor binding
zinc ion binding
enzyme binding
transcription regulatory region DNA binding
protein dimerization activity
ATPase binding
protein domain specific binding
sequence-specific DNA binding
androgen receptor binding
POU domain binding
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
transport
signal transduction
cell-cell signaling
sex differentiation
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of gene expression
cell growth
androgen receptor signaling pathway
intracellular receptor signaling pathway
prostate gland development
positive regulation of phosphorylation
positive regulation of cell differentiation
negative regulation of integrin biosynthetic process
positive regulation of integrin biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
regulation of establishment of protein localization to plasma membrane
negative regulation of extrinsic apoptotic signaling pathway
in utero embryonic development
regulation of systemic arterial blood pressure
epithelial cell morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatogenesis
single fertilization
male gonad development
fertilization
cellular process
regulation of gene expression
male somatic sex determination
response to insulin
positive regulation of intracellular estrogen receptor signaling pathway
Leydig cell differentiation
multicellular organism growth
steroid hormone mediated signaling pathway
positive regulation of MAPK cascade
positive regulation of insulin-like growth factor receptor signaling pathway
male sex differentiation
reproductive structure development
regulation of developmental growth
organ formation
male genitalia morphogenesis
negative regulation of epithelial cell proliferation
regulation of catalytic activity
activation of prostate induction by androgen receptor signaling pathway
morphogenesis of an epithelial fold
lateral sprouting involved in mammary gland duct morphogenesis
regulation of prostatic bud formation
prostate gland growth
prostate gland epithelium morphogenesis
epithelial cell differentiation involved in prostate gland development
tertiary branching involved in mammary gland duct morphogenesis
mammary gland alveolus development
reproductive system development
cellular response to steroid hormone stimulus
seminiferous tubule development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support