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Invitrogen
Androgen Receptor Recombinant Rabbit Monoclonal Antibody (23GB3210)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Androgen Receptor Antibody (MA5-53348) in ICC/IF
Immunocytochemistry analysis of Androgen Receptor in C2C12 cells. Nuclei were stained blue with DAPI; Androgen Receptor recombinant monoclonal antibody (Product # MA5-53348) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.
Product Details
MA5-53348
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB3210
Immunogen
A synthetic peptide of human Androgen Receptor (3-65AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: androgen nuclear receptor variant 2; Androgen receptor; androgen receptor splice variant 4b; AR-A; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; Variant AR45
Gene Aliases: AIS; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
UniProt ID: (Human) P10275, (Mouse) P19091
Entrez Gene ID: (Human) 367, (Mouse) 11835
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
androgen receptor activity
receptor binding
steroid binding
androgen binding
protein binding
beta-catenin binding
transcription factor binding
zinc ion binding
enzyme binding
transcription regulatory region DNA binding
protein dimerization activity
ATPase binding
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
transport
signal transduction
cell-cell signaling
sex differentiation
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
positive regulation of gene expression
cell growth
androgen receptor signaling pathway
intracellular receptor signaling pathway
prostate gland development
positive regulation of phosphorylation
positive regulation of cell differentiation
negative regulation of integrin biosynthetic process
positive regulation of integrin biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
regulation of establishment of protein localization to plasma membrane
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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