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Angiopoietin 1 Antibody (100-401-403) in IHC (P)
Rocklands anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Product Details
100-401-403
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Conjugate
Form
Liquid
Concentration
80 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.
Target Information
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANG-1; Angiopoietin-1
Gene Aliases: 1110046O21Rik; AGP1; AGPT; Ang-1; ANG1; ANGPT1; KIAA0003
UniProt ID: (Human) Q15389, (Mouse) O08538
Entrez Gene ID: (Human) 284, (Mouse) 11600
MAPK cascade
in utero embryonic development
negative regulation of protein phosphorylation
regulation of endothelial cell proliferation
sprouting angiogenesis
positive regulation of receptor internalization
negative regulation of cytokine secretion involved in immune response
negative regulation of cell adhesion
activation of transmembrane receptor protein tyrosine kinase activity
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
regulation of skeletal muscle satellite cell proliferation
hemopoiesis
cell differentiation
heparin biosynthetic process
positive regulation of protein ubiquitination
cell-substrate adhesion
regulation of tumor necrosis factor production
positive regulation of peptidyl-serine phosphorylation
protein localization to cell surface
negative regulation of protein import into nucleus
negative regulation of apoptotic process
negative regulation of vascular permeability
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein binding
negative regulation of neuron apoptotic process
positive regulation of blood vessel endothelial cell migration
positive regulation of GTPase activity
positive regulation of cell adhesion
Tie signaling pathway
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
glomerulus vasculature development
negative regulation of endothelial cell apoptotic process
regulation of macrophage migration inhibitory factor signaling pathway
angiogenesis
ovarian follicle development
patterning of blood vessels
vasculogenesis
endocardium morphogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
endoderm development
positive regulation of vascular endothelial growth factor receptor signaling pathway
protein homooligomerization
cardiac muscle tissue morphogenesis
vasculogenesis involved in coronary vascular morphogenesis
Performance Guarantee
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