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Invitrogen
Angiotensinogen Recombinant Rabbit Monoclonal Antibody (034)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Angiotensinogen Antibody (MA5-29010) in ICC/IF
Immunofluorescence analysis of Angiotensinogen was performed using 70% confluent log phase Hep G2 cells treated with 1X protein transport inhibitor (Product # 00-4980-03) for 4 hours. Angiotensinogen is readily secreted out in the medium after translation and post processing. Treatment of cells with protein transport inhibitor sequesters Angiotensinogen in the endoplasmic reticulum. The cells were fixed wit... View More
Product Details
MA5-29010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
034
Immunogen
Recombinant Human SerpinA8/Angiotensinogen/AGT protein (Met1-Ala485)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784948
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human SerpinA8/Angiotensinogen/AGT.
Target Information
The protein encoded by AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-1 antiproteinase, antitrypsin; angiotensin I; angiotensin II; Angiotensinogen; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; serpin peptidase inhibitor, clade A, member 8
Gene Aliases: AGT; ANHU; SERPINA8
UniProt ID: (Human) P01019
Entrez Gene ID: (Human) 183
regulation of cell growth
positive regulation of cytokine production
kidney development
blood vessel remodeling
angiotensin maturation
regulation of blood volume by renin-angiotensin
renin-angiotensin regulation of aldosterone production
regulation of renal output by angiotensin
regulation of heart rate
regulation of blood vessel size by renin-angiotensin
renal system process
angiotensin-mediated drinking behavior
positive regulation of extracellular matrix constituent secretion
cellular sodium ion homeostasis
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
phospholipase C-activating G-protein coupled receptor signaling pathway
activation of phospholipase C activity
nitric oxide mediated signal transduction
cell-cell signaling
female pregnancy
aging
regulation of blood pressure
positive regulation of activation of JAK2 kinase activity
positive regulation of endothelial cell migration
positive regulation of cardiac muscle hypertrophy
positive regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol esterification
negative regulation of endopeptidase activity
regulation of norepinephrine secretion
positive regulation of phosphatidylinositol 3-kinase signaling
artery smooth muscle contraction
response to muscle activity involved in regulation of muscle adaptation
negative regulation of angiogenesis
regulation of vasoconstriction
negative regulation of cell growth
positive regulation of cellular protein metabolic process
positive regulation of superoxide anion generation
positive regulation of NAD(P)H oxidase activity
negative regulation of tissue remodeling
low-density lipoprotein particle remodeling
catenin import into nucleus
regulation of renal sodium excretion
positive regulation of renal sodium excretion
regulation of cell proliferation
vasodilation
positive regulation of nitric oxide biosynthetic process
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of blood pressure
positive regulation of transcription, DNA-templated
positive regulation of vasodilation
fibroblast proliferation
positive regulation of fibroblast proliferation
regulation of long-term neuronal synaptic plasticity
smooth muscle cell proliferation
cytokine secretion
positive regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of NF-kappaB transcription factor activity
negative regulation of neurotrophin TRK receptor signaling pathway
stress-activated MAPK cascade
regulation of calcium ion transport
regulation of transmission of nerve impulse
cell growth involved in cardiac muscle cell development
positive regulation of protein tyrosine kinase activity
ERK1 and ERK2 cascade
uterine smooth muscle contraction
cellular response to mechanical stimulus
positive regulation of branching involved in ureteric bud morphogenesis
regulation of extracellular matrix assembly
positive regulation of gap junction assembly
regulation of cardiac conduction
positive regulation of reactive oxygen species metabolic process
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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