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Ankyrin G Antibody (MA5-45540) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Ankyrin G monoclonal antibody (Product # MA5-45540) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 fo... View More
Product Details
MA5-45540
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N106/20
Immunogen
Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931994
Product Specific Information
1 µg/mL of MA5-45540 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects >approximately 200kDa.
This antibody was formerly sold as clone S106-20
Target Information
Ankyrin-G is found in several tissues including brain where, due to tissue-specific RNA processing, it is expressed as two different isoforms, localized to axon initial segments and nodes of Ranvier. Ankyrin-G is also found at sites of epithelial cell-cell contact and in the intracellular membranes of some cells. Ankyrin-G isoforms contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following a globular head domain. Ankyrin-G is thought to participate in the maintenance and targeting of ion channels and cell adhesion molecules to the nodes of Ranvier and to axon initial segments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANK-3; ankyrin 3 (G); ankyrin 3, epithelial; Ankyrin-3; Ankyrin-G; brain-specific ankyrin-G
Gene Aliases: 2900054D09Rik; AI314020; ANK-3; ANK3; AnkG; Ankyrin-3; ANKYRIN-G; MRT37
UniProt ID: (Human) Q12955, (Rat) O70511, (Mouse) G5E8K5
Entrez Gene ID: (Human) 288, (Rat) 361833, (Mouse) 11735
mitotic cytokinesis
ER to Golgi vesicle-mediated transport
plasma membrane organization
cytoskeletal anchoring at plasma membrane
signal transduction
axonogenesis
axon guidance
neuromuscular junction development
positive regulation of gene expression
positive regulation of cell communication by electrical coupling
positive regulation of sodium ion transport
magnesium ion homeostasis
neuronal action potential
positive regulation of homotypic cell-cell adhesion
Golgi to plasma membrane protein transport
regulation of potassium ion transport
establishment of protein localization
positive regulation of membrane potential
cellular response to magnesium ion
membrane assembly
protein localization to plasma membrane
maintenance of protein location in plasma membrane
protein targeting to plasma membrane
positive regulation of protein targeting to membrane
positive regulation of membrane depolarization during cardiac muscle cell action potential
negative regulation of delayed rectifier potassium channel activity
positive regulation of sodium ion transmembrane transporter activity
positive regulation of cation channel activity
synapse organization
positive regulation of action potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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