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Invitrogen
Arginase 1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Arginase 1 Antibody (PA5-32267) in IHC (P)
Immunohistochemical (paraffin) analysis of Arginase-1 using anti-Arginase-1 Polyclonal Antibody (Product # PA5-32267) in Hepatocellular Carcinoma Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:100.
Product Details
PA5-32267
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminus of human arginase-1 protein.
Conjugate
Form
Liquid
Concentration
0.046 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2549740
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is hepatocellular carcinoma.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF
UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176
Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine catabolic process
aging
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to cadmium ion
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
arginine metabolic process
female pregnancy
response to wounding
response to lipopolysaccharide
response to peptide hormone
response to steroid hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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