Invitrogen

Aromatase Polyclonal Antibody

4 Published Figures

View all (23) Aromatase antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

Aromatase Antibody in Western Blot (WB)

Aromatase Antibody in Western Blot (WB)

FIGURE: 1 / 5

Aromatase Antibody (PA1-16532) in WB

Western blot analysis of Aromatase in human fetal temporal lobe lysate. Samples were incubated in Aromatase polyclonal antibody (Product # PA1-16532). ECL exposure, 1-2 minutes.

Aromatase Antibody in Western Blot (WB)

Aromatase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Aromatase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Aromatase Antibody in Immunocytochemistry (ICC/IF)

Aromatase Antibody in Western Blot (WB)

Product Image Gallery

Aromatase Antibody in Western Blot (WB) — Western blot analysis of Aromatase in human fetal temporal lobe lysate. Samples were incubated in Aromatase polyclonal antibody (Product # PA1-16532). ECL exposure, 1-2 minutes.

Aromatase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 7. Male FBN-ARO-KO mice exhibit strong neurodegeneration and cognitive impairment 7 d after GCI injury. Aa , Astrocyte activation and aromatization in hippocampal CA1 region were examined by GFAP and aromatase double staining. Ab , GFAP levels in each group were quantified as a parameter of astrocyte reactivity after GCI injury. Total aromatase levels in hippocampal CA1 region were further measured. B , Astrocyte A1 and A2 phenotypes after GCI were examined by IHC analysis with the markers of C3D ( Ba ) and S100A10 ( Bb ), respectively. Ca , BDNF, IGF-1, and GLT-1 production in astrocytes following the altered astrocyte activation was determined by IHC analysis. Cb , Their relative changes of intensities in astrocytes were quantified. Da , Representative double staining for NeuN and F-Jade C to evaluate neuronal degeneration in each group. Db , F-Jade C-positive neurons were counted. E , Spatial reference memory was assessed by Barnes Maze behavioral test. Ea-Ed , Tracking plots in probe trial. Primary escape latency ( Ee ) and quadrant occupancy ( Ef ) of animals from each group in probe trial were recorded. Values are mean +- SEM of determinations from each group. N = 4 for A-C ; N = 8-11 for D . * p < 0.05 versus FLOX+Sham. # p < 0.05 versus FLOX+GCI.

Aromatase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 1. Attenuated astrocyte activation and aromatization in ovariectomized female FBN-ARO-KO mice after GCI. A , IHC analysis for alterations in astrocyte reactivity and aromatase induction in hippocampal CA1 region at 3, 7, and 14 d after GCI. B , Measurement of hippocampal E2 levels with high-sensitivity E2 ELISA kit at 7 d after GCI. Ca , Representative 3D images of hippocampal CA1 astrocytes with cell body volumes distinguished with different colors. Cb , Astrocyte volumes from the indicated groups were measured and quantitatively analyzed. D , Astrocyte activation was confirmed by examining the expression of two typical astrocyte markers, GFAP and vimentin, with Western blot analysis. E , Astrocyte numbers in hippocampal CA1 region were counted with both GFAP and S100beta immunostaining and expressed as cell numbers per 10 5 um 2 . Values are mean +- SEM of determinations from each group. N = 3-5. * p < 0.05 versus FLOX+Sham. $ p < 0.05 versus FLOX + 3 d. # p < 0.05 versus FLOX + 7 d. & p < 0.05 versus FLOX + 14 d. NS, no significant difference.

Aromatase Antibody in Immunocytochemistry (ICC/IF) — Figure 8. Male FBN-ARO-KO mice display attenuated astrocyte activation, compromised astrocyte functions, and worse neuronal damage at 3 and 14 d after GCI. A , B , GFAP and aromatase double staining to examine astrocyte activation and astrocytic aromatase induction at 3 d ( Aa ) and 14 d ( Ba ) after GCI. Relative changes of GFAP intensities at 3d ( Ab ) and 14d ( Bb ) were quantified. C , Astrocyte-derived BDNF ( Ca ), IGF-1 ( Cb ), and GLT-1 ( Cc ) levels at 3 d after GCI were explored by IHC analysis. D , Their relative intensities in KO + 3 d mice versus FLOX + 3 d mice. E , F , BDNF ( Ea ), IGF-1 ( Eb ), and GLT-1 ( Ec ) levels in hippocampal astrocytes at 14 d after GCI were determined with IHC, and expressed as relative changes of KO + 14 d mice compared with FLOX + 14 d mice ( F ). G , H , Neuronal damage in hippocampal CA1 region at 3 d ( G ) and 14 d ( H ) after GCI was assessed by NeuN and F-Jade C double staining. Values are mean +- SEM of determinations from each group. N = 4. # p < 0.05 versus FLOX + 3 d. & p < 0.05 versus FLOX + 14 d.

Aromatase Antibody in Western Blot (WB) — Figure 9. Evidence that upregulation of neuronal FGF2 signaling mediates decreased astrocyte activation in ovariectomized female FBN-ARO-KO mice after GCI. Aa , Representative image of FGF2 and NeuN double staining in hippocampal CA1 region. Ab , Quantitative analysis of neuronal FGF2 levels in each group ( N = 4 or 5). B , Neuronal FGF2 alterations in hippocampus were confirmed by Western blotting analysis ( N = 3). C , Representative images of FGFR3 and GFAP double staining showing changes of FGFR3 expression in hippocampal CA1 astrocytes ( N = 4 or 5). Da , Schematic illustration of FGFR3 neutralization by bilateral intracerebroventricular injection of FGFR3 blocking antibody in FBN-ARO-KO GCI mice. Db , The purity of isolated astrocytes from the ischemic brains was evaluated by flow cytometry analysis. E , To examine the effects of FGFR3 neutralization on astrocyte reactivity and functional restoration in FBN-ARO-KO mice after GCI, levels of GFAP, p-STAT3, BDNF, and GLT-1 in purified astrocytes were determined by Western blotting ( Ea ), and further quantitatively analyzed ( Eb ) ( N = 3). Fa , IHC analysis for neuronal degeneration by F-Jade C and NeuN double staining in hippocampal CA1 region. Fb , Quantification of F-Jade C levels in CA1 pyramidal neurons, which was presented as relative intensity of each group versus FLOX+GCI ( N = 4 or 5). Values are mean +- SEM of determinations from each group. * p < 0.05 versus FLOX+Sham. # p < 0.05 versus FLOX+GCI. $ p < 0.05 ver

Product Details

PA1-16532

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to a C-terminal portion of the human aromatase protein sequence (between residues 400-502).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_568446

Product Specific Information

This antibody is expected to react with equine (91%), bovine and rabbit (90%), porcine (83%), ovine, goat and canine (81%) based on sequence homology.

Suggested positive control: antigen standard for CYP19A1 (transient overexpression lysate), human Fetal Temporal Lobe protein.

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-16532 was used in Immunohistochemistry (Frozen) to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-16532 was used in Immunohistochemistry (Frozen) to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-16532 was used in Immunohistochemistry (Frozen) to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lu Y,Sareddy GR,Wang J,Zhang Q,Tang FL,Pratap UP,Tekmal RR,Vadlamudi RK,Brann DW

PA1-16532 was used in Immunohistochemistry (Frozen) to demonstrate neuron-derived 17β-estradiol has a neuroprotective role during ischemic brain injury.

Wed Sep 16 00:00:00 EDT 2020

Astrocyte-Derived Estrogen Regulates Reactive Astrogliosis and is Neuroprotective following Ischemic Brain Injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wang J,Sareddy GR,Lu Y,Pratap UP,Tang F,Greene KM,Meyre PL,Tekmal RR,Vadlamudi RK,Brann DW

PA1-16532 was used in Immunohistochemistry (Frozen) to investigate if astrocyte-derived E2 has a key role in the regulation of reactive astrogliosis, microglial activation, and neuroprotection after cerebral ischemia.

Wed Dec 09 00:00:00 EST 2020

Alteration in expression of estrogen receptor isoforms alpha and beta, and aromatase in the testis and its relation with changes in nitric oxide during aging in mice.

Steroids

Banerjee A,Anjum S,Verma R,Krishna A

PA1-16532 was used in immunohistochemistry and western blot to study testicular aromatase, estrogen receptor-alpha, estrogen receptor-beta and iNOS expression and the relationship with serum testosterone and nitric oxide levels during murine ageing

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; cytochrome P450 family 19 subfamily a; Cytochrome P450, 19, aromatase; cytochrome P450, family 19, subfamily A, polypeptide 1; cytochrome P450, subfamily 19; cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; MGC104309; microsomal monooxygenase; P-450AROM

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Gene Aliases: Ar; ArKO; ARO; ARO1; Arom; Aromatase; CPV1; CYAR; CYP19; Cyp19a; CYP19A1; CYPXIX; Int-5; Int5; P-450AROM; p450arom

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UniProt ID: (Human) P11511, (Rabbit) Q29605, (Mouse) P28649

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Entrez Gene ID: (Human) 1588, (Rabbit) 100328545, (Mouse) 13075, (Rat) 25147

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iron ion binding steroid hydroxylase activity electron carrier activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen oxygen binding heme binding aromatase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxygenase

Process(es)

negative regulation of chronic inflammatory response steroid biosynthetic process estrogen biosynthetic process androgen metabolic process negative regulation of macrophage chemotaxis sterol metabolic process oxidation-reduction process prostate gland growth C21-steroid hormone metabolic process negative regulation of bone resorption regulation of synapse structure or activity luteinization bile acid metabolic process male gonad development response to herbicide response to metal ion response to cesium ion response to ionizing radiation response to organic cyclic compound biphenyl metabolic process dibenzo-p-dioxin metabolic process phthalate metabolic process hippocampus development hypothalamus development melatonin metabolic process adrenal gland development granulocyte differentiation response to estradiol response to testosterone progesterone metabolic process response to drug response to morphine response to estrogen cellular response to fibroblast growth factor stimulus bone development cellular response to glucose stimulus cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus cellular response to hydrostatic pressure

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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