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Aspartate Transaminase Antibody (200-603-144-0100) in DB
Dot Blot of Sheep anti-Aspartate Transaminase Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Sh-a-Aspartate Transaminase (AST) HRP 200-603-144 at 1µg/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT.
Product Details
200-603-144-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Aspartate Aminotransferase (AST) [Pig Heart]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
Contains
0.01% gentamicin sulfate
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]. Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.
Target Information
GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aspartate aminotransferase, cytoplasmic; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); Transaminase A
Gene Aliases: CASPAT; cCAT; GOT1
UniProt ID: (Pig) P00503
Entrez Gene ID: (Pig) 396967
2-oxoglutarate metabolic process
oxaloacetate metabolic process
glycerol biosynthetic process
aspartate metabolic process
aspartate catabolic process
glutamate metabolic process
Notch signaling pathway
glutamate catabolic process to aspartate
glutamate catabolic process to 2-oxoglutarate
cellular response to insulin stimulus
response to glucocorticoid
fatty acid homeostasis
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