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Invitrogen
Ataxin 3 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Product Details
PA5-22369
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (Uniprot ID#P54252)
Conjugate
Form
Liquid
Concentration
0.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11152091
Product Specific Information
Recommended positive controls: SK-N-SH, SK-N-AS, mouse brain, human Ataxin3-transfected 293T.
Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (85%), Pig (99%), Chicken (89%), Sheep (98%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ataxin 3 variant an; ataxin 3 variant ao; ataxin 3 variant at; ataxin 3 variant e; ataxin 3 variant h; ataxin 3 variant m; ataxin 3 variant r; ataxin 3 variant ref; ataxin 3 variant y; Ataxin-3; ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3); Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein
Gene Aliases: 2210008M02Rik; AI463012; AI647473; AT3; ataxin-3; ATX3; ATXN3; JOS; MJD; MJD1; SCA3
UniProt ID: (Human) P54252, (Mouse) Q9CVD2
Entrez Gene ID: (Human) 4287, (Mouse) 110616
RNA polymerase II regulatory region DNA binding
histone deacetylase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
omega peptidase activity
ubiquitin protein ligase binding
identical protein binding
ATPase binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
chromatin/chromatin-binding, or -regulatory protein
DNA metabolism protein
microtubule cytoskeleton organization
nucleotide-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
chemical synaptic transmission
nervous system development
regulation of cell-substrate adhesion
protein deubiquitination
actin cytoskeleton organization
cellular response to heat
monoubiquitinated protein deubiquitination
exploration behavior
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
protein K63-linked deubiquitination
histone H3 deacetylation
protein K48-linked deubiquitination
cellular response to misfolded protein
positive regulation of ERAD pathway
protein localization to cytosolic proteasome complex involved in ERAD pathway
proteolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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