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Invitrogen
Ataxin 3 Recombinant Rabbit Monoclonal Antibody (7B2I1)
FIGURE: 1 / 1
Ataxin 3 Antibody (MA5-51244) in WB
Western blot analysis of Ataxin 3 in extracts from various lysates. Samples were incubated with Ataxin 3 Recombinant monoclonal antibody (Product # MA5-51244) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-51244
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7B2I1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-361 of human Ataxin-3 (ATXN3) (NP_004984.2).
Conjugate
Form
Liquid
Concentration
0.53 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093889
Product Specific Information
Immunogen sequence: KLIGEELAQL KEQRVHKTDL ERVLEANDGS GMLDEDEEDL QRALALSRQE IDMEDEEADL RRAIQLSMQG SSRNISQDMT QTSGTNLTSE ELRKRREAYF EKQQQKQQQQ QQQQQQGDLS GQSSHPCERP ATSSGALGSD LGDAMSEEDM LQAAVTMSLE TVRNDLKTEG KK
Target Information
Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ataxin-3; ataxin3; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1 homolog; spin
Gene Aliases: 2210008M02Rik; AI463012; AI647473; ataxin-3; ATX3; Atxn3; Mjd; MJD1; Rsca3; Sca3
UniProt ID: (Rat) O35815, (Mouse) Q9CVD2
Entrez Gene ID: (Rat) 60331, (Mouse) 110616
RNA polymerase II regulatory region DNA binding
histone deacetylase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
omega peptidase activity
ubiquitin protein ligase binding
identical protein binding
ATPase binding
Lys63-specific deubiquitinase activity
Lys48-specific deubiquitinase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
chromatin/chromatin-binding, or -regulatory protein
microtubule cytoskeleton organization
transcription, DNA-templated
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
misfolded or incompletely synthesized protein catabolic process
regulation of cell-substrate adhesion
actin cytoskeleton organization
cellular response to heat
monoubiquitinated protein deubiquitination
exploration behavior
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
protein K63-linked deubiquitination
histone H3 deacetylation
protein K48-linked deubiquitination
cellular response to misfolded protein
proteolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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