Proteintech

B23/NPM1 Monoclonal Antibody (4F12A3)

B23/NPM1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

B23/NPM1 Antibody (60096-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 60096-1-IG (B23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

B23/NPM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

60096-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:50,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:200

View 13 publications 13 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F12A3

Immunogen

B23/NPM1 Fusion Protein Ag7415 (1-293 aa encoded by BC002398)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEDSMDMDM SPLRPQNYLF GCELKADKDY HFKVDNDENE HQLSLRTVSL GAGAKDELHI VEAEAMNYEG SPIKVTLATL KMSVQPTVSL GGFEITPPVV LRLKCGSGPV HISGQHLVAV EEDAESEDEE EEDVKLLSIS GKRSAPGGGS KVPQKKVKLA ADEDDDDDDE EDDDEDDDDD DFDDEEAEEK APVKKSIRDT PAKNAQKSNQ NGKDSKPSST PRSKGQESFK KQEKTPKTPK GPSSVEDIKA KMQASIEKGG SLPKVEAKFI NYVKNCFRMT DQEAIQDLWQ WRKS (1-293 aa encoded by BC002398)

Target Information

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation.

Brain : a journal of neurology

Fan Y,Li MJ,Yang J,Li SJ,Hao XY,Li JD,Wang YC,Tang MB,Zhang C,Shi JJ,Ma DR,Guo MN,Liu F,Shen S,Yao DB,Zuo CY,Mao CY,Hu ZW,Zhang S,Yang ZH,Guo GY,Yang JH,Xia ZP,Xu YM,Shi CH

Tue Aug 01 00:00:00 EDT 2023

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

iScience

Ghanam AR,Ke S,Wang S,Elgendy R,Xie C,Wang S,Zhang R,Wei M,Liu W,Cao J,Zhang Y,Zhang Z,Xue T,Zheng Y,Song X

Fri Jan 20 00:00:00 EST 2023

Single-cell transcriptome analyses reveal critical roles of RNA splicing during leukemia progression.

PLoS biology

Wu B,Chen X,Pan X,Deng X,Li S,Wang Z,Wang J,Liao D,Xu J,Chen M,Zhao C,Xue Z,Wang Y,Niu T,Lin J,Chen L,Liu Y,Chen C

Mon May 01 00:00:00 EDT 2023

Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in NPM1-mutated acute myeloid leukemia.

Acta pharmaceutica Sinica. B

Feng Y,Han Y,Hu A,Qu Y,Hu Y,Wu H,Wang X,He L

Wed Feb 01 00:00:00 EST 2023

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

International journal of oncology

Wang R,Peng C,Song J,Hua Y,Wu Q,Deng L,Cao Y,Zhang J,Zhang L,Wu L,Hou L

Tue Mar 01 00:00:00 EST 2022

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

International journal of oncology

Wang R,Peng C,Song J,Hua Y,Wu Q,Deng L,Cao Y,Zhang J,Zhang L,Wu L,Hou L

Tue Mar 01 00:00:00 EST 2022

NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance.

Cell death discovery

Shi J,Chen SY,Shen XT,Yin XK,Zhao WW,Bai SM,Feng WX,Feng LL,Qin C,Zheng J,Wang YL,Fan XJ

Mon Oct 31 00:00:00 EDT 2022

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Cell death discovery

Wang F,Cheng C,Wang X,Chen F,Li H,Zhou Y,Wang Y,Hu X,Kong P,Zhang L,Cheng X,Cui Y

Fri Apr 08 00:00:00 EDT 2022

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Cell death discovery

Wang F,Cheng C,Wang X,Chen F,Li H,Zhou Y,Wang Y,Hu X,Kong P,Zhang L,Cheng X,Cui Y

Fri Apr 08 00:00:00 EDT 2022

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Cell death discovery

Wang F,Cheng C,Wang X,Chen F,Li H,Zhou Y,Wang Y,Hu X,Kong P,Zhang L,Cheng X,Cui Y

Fri Apr 08 00:00:00 EDT 2022

Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma.

Cell death discovery

Wang F,Cheng C,Wang X,Chen F,Li H,Zhou Y,Wang Y,Hu X,Kong P,Zhang L,Cheng X,Cui Y

Fri Apr 08 00:00:00 EDT 2022

The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Cell biochemistry and function

Xu SX,Han YW,Guo JL,Bian XK,Hu HM,Lee SC

Fri Jul 01 00:00:00 EDT 2022

The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Cell biochemistry and function

Xu SX,Han YW,Guo JL,Bian XK,Hu HM,Lee SC

Fri Jul 01 00:00:00 EDT 2022

The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification.

Cell biochemistry and function

Xu SX,Han YW,Guo JL,Bian XK,Hu HM,Lee SC

Fri Jul 01 00:00:00 EDT 2022

NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.

The international journal of biochemistry & cell biology

Wang D,Li Y,Liu Y,Cheng S,Liu F,Zuo R,Ding C,Shi S,Liu G

Wed Jun 01 00:00:00 EDT 2022

Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.

Aging cell

Gomez-Suaga P,Mórotz GM,Markovinovic A,Martín-Guerrero SM,Preza E,Arias N,Mayl K,Aabdien A,Gesheva V,Nishimura A,Annibali A,Lee Y,Mitchell JC,Wray S,Shaw C,Noble W,Miller CCJ

Tue Feb 01 00:00:00 EST 2022

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Science advances

Cao P,Yang A,Li P,Xia X,Han Y,Zhou G,Wang R,Yang F,Li Y,Zhang Y,Cui Y,Ji H,Lu L,He F,Zhou G

Sun Aug 01 00:00:00 EDT 2021

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Cell death & disease

Luo W,Li J,Li Z,Lin T,Zhang L,Yang W,Mai Y,Liu R,Chen M,Dai C,Yang H,Lu J,Li H,Guan G,Huang M,Liu P,Li Z

Mon Jul 26 00:00:00 EDT 2021

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Cell death & disease

Luo W,Li J,Li Z,Lin T,Zhang L,Yang W,Mai Y,Liu R,Chen M,Dai C,Yang H,Lu J,Li H,Guan G,Huang M,Liu P,Li Z

Mon Jul 26 00:00:00 EDT 2021

HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.

Cell death & disease

Luo W,Li J,Li Z,Lin T,Zhang L,Yang W,Mai Y,Liu R,Chen M,Dai C,Yang H,Lu J,Li H,Guan G,Huang M,Liu P,Li Z

Mon Jul 26 00:00:00 EDT 2021

Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.

Acta biochimica et biophysica Sinica

Chen F,Yan H,Guo C,Zhu H,Yi J,Sun X,Yang J

Tue Aug 31 00:00:00 EDT 2021

APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

In vitro cellular & developmental biology. Animal

Xia H,Liu Y,Xu L,Mao H,Zhou Q,Xie Y

Sat May 01 00:00:00 EDT 2021

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Molecular neurodegeneration

Zhang Q,Zhang J,Ye J,Li X,Liu H,Ma X,Wang C,He K,Zhang W,Yuan J,Zhao Y,Xu H,Liu Q

Wed Sep 22 00:00:00 EDT 2021

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Molecular neurodegeneration

Zhang Q,Zhang J,Ye J,Li X,Liu H,Ma X,Wang C,He K,Zhang W,Yuan J,Zhao Y,Xu H,Liu Q

Wed Sep 22 00:00:00 EDT 2021

The protease activity of human ATG4B is regulated by reversible oxidative modification.

Autophagy

Zheng X,Yang Z,Gu Q,Xia F,Fu Y,Liu P,Yin XM,Li M

Thu Oct 01 00:00:00 EDT 2020

Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.

Heliyon

Bennett CL,Sopher BL,La Spada AR

Mon Jun 01 00:00:00 EDT 2020

Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.

Nature metabolism

Li ST,Huang D,Shen S,Cai Y,Xing S,Wu G,Jiang Z,Hao Y,Yuan M,Wang N,Zhu L,Yan R,Yang D,Wang L,Liu Z,Hu X,Zhou R,Qu K,Li A,Duan X,Zhang H,Gao P

Sun Mar 01 00:00:00 EST 2020

MYCT1 represses apoptosis of laryngeal cancerous cells through the MAX/miR-181a/NPM1 pathway.

The FEBS journal

Wang HT,Tong X,Zhang ZX,Sun YY,Yan W,Xu ZM,Fu WN

Tue Oct 01 00:00:00 EDT 2019

Identification of the key genes and pathways in prostate cancer.

Oncology letters

Fan S,Liang Z,Gao Z,Pan Z,Han S,Liu X,Zhao C,Yang W,Pan Z,Feng W

Thu Nov 01 00:00:00 EDT 2018

Identification of the key genes and pathways in prostate cancer.

Oncology letters

Fan S,Liang Z,Gao Z,Pan Z,Han S,Liu X,Zhao C,Yang W,Pan Z,Feng W

Thu Nov 01 00:00:00 EDT 2018

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Nature communications

Wu G,Yuan M,Shen S,Ma X,Fang J,Zhu L,Sun L,Liu Z,He X,Huang D,Li T,Li C,Wu J,Hu X,Li Z,Song L,Qu K,Zhang H,Gao P

Fri May 05 00:00:00 EDT 2017

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Nature communications

Wu G,Yuan M,Shen S,Ma X,Fang J,Zhu L,Sun L,Liu Z,He X,Huang D,Li T,Li C,Wu J,Hu X,Li Z,Song L,Qu K,Zhang H,Gao P

Fri May 05 00:00:00 EDT 2017

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Nature communications

Yang K,Wang M,Zhao Y,Sun X,Yang Y,Li X,Zhou A,Chu H,Zhou H,Xu J,Wu M,Yang J,Yi J

Fri Nov 25 00:00:00 EST 2016

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Nature communications

Yang K,Wang M,Zhao Y,Sun X,Yang Y,Li X,Zhou A,Chu H,Zhou H,Xu J,Wu M,Yang J,Yi J

Fri Nov 25 00:00:00 EST 2016

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Nature communications

Yang K,Wang M,Zhao Y,Sun X,Yang Y,Li X,Zhou A,Chu H,Zhou H,Xu J,Wu M,Yang J,Yi J

Fri Nov 25 00:00:00 EST 2016

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Nature communications

Yang K,Wang M,Zhao Y,Sun X,Yang Y,Li X,Zhou A,Chu H,Zhou H,Xu J,Wu M,Yang J,Yi J

Fri Nov 25 00:00:00 EST 2016

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhu Y,Cai L,Guo J,Chen N,Yi X,Zhao Y,Cai J,Wang Z

Sat Oct 01 00:00:00 EDT 2016

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Journal of molecular biology

Szerlong HJ,Herman JA,Krause CM,DeLuca JG,Skoultchi A,Winger QA,Prenni JE,Hansen JC

Fri Jun 05 00:00:00 EDT 2015

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Journal of molecular biology

Szerlong HJ,Herman JA,Krause CM,DeLuca JG,Skoultchi A,Winger QA,Prenni JE,Hansen JC

Fri Jun 05 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Human molecular genetics

Tao Z,Wang H,Xia Q,Li K,Li K,Jiang X,Xu G,Wang G,Ying Z

Fri May 01 00:00:00 EDT 2015

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

PloS one

Jiang WQ,Nguyen A,Cao Y,Chang AC,Reddel RR

Tue Feb 15 00:00:00 EST 2011

HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a.

PloS one

Jiang WQ,Nguyen A,Cao Y,Chang AC,Reddel RR

Tue Feb 15 00:00:00 EST 2011

Bioinformatics

Protein Aliases: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; nucleolar protein B23.1; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128

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Gene Aliases: B23; B23NP; NO38; NPM; NPM1

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UniProt ID: (Human) P06748, (Rat) P13084, (Mouse) Q61937

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Entrez Gene ID: (Human) 4869, (Rat) 25498, (Mouse) 18148

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Function(s)

transcription coactivator activity RNA binding protein kinase inhibitor activity protein binding protein kinase binding Tat protein binding histone binding protein homodimerization activity ribosomal large subunit binding ribosomal small subunit binding poly(A) RNA binding protein heterodimerization activity NF-kappaB binding unfolded protein binding DNA binding phosphatidylinositol-3,4,5-trisphosphate binding rRNA binding enzyme binding nucleic acid binding protein N-terminus binding chaperone

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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B23/NPM1 Monoclonal Antibody (4F12A3)

B23/NPM1 Antibody (60096-1-IG) in ICC/IF

Product Details

60096-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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