Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Biotin

BrdU Antibody in Immunohistochemistry (IHC)

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BrdU Antibody (B35138) in IHC

Journal of neuromuscular diseases 2021 - Muscle degeneration differentially affects turnover of muscle proteins in treated mdx mice. A-B) Quantification of BrdU-labeled fibers as a measure of muscle degeneration over time from baseline for mdx . WT cohorts exhibited almost no regeneration as marked by BrdU incorporation in all WT cohorts. BrdU-positive fibers were quantified per mm 2 for the quadriceps, triceps and tibialis anterior at each end po... View More

BrdU Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

B35138

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536438

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A FoxA2+ long-term stem cell population is necessary for growth plate cartilage regeneration after injury.

Nature communications

Muruganandan S,Pierce R,Teguh DA,Perez RF,Bell N,Nguyen B,Hohl K,Snyder BD,Grinstaff MW,Alberico H,Woods D,Kong Y,Sima C,Bhagat S,Ho K,Rosen V,Gamer L,Ionescu AM

B35138 was used in Immunohistochemistry to show that when the growth plate is injured, FoxA2+ cells expand in response to trauma, and produce physeal cartilage for growth plate tissue regeneration.

Fri May 06 00:00:00 EDT 2022

Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy.

Journal of neuromuscular diseases

Novak JS,Spathis R,Dang UJ,Fiorillo AA,Hindupur R,Tully CB,Mázala DAG,Canessa E,Brown KJ,Partridge TA,Hathout Y,Nagaraju K

B35138 was used in Immunohistochemistry to demonstrate the use of targeted mass spectrometry to evaluate the suitability and functionality of restored dystrophin isoforms in the context of disease and propose its use to optimize alternative gene correction strategies in development for DMD.

Fri Dec 17 00:00:00 EST 2021

Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy.

Journal of neuromuscular diseases

Novak JS,Spathis R,Dang UJ,Fiorillo AA,Hindupur R,Tully CB,Mázala DAG,Canessa E,Brown KJ,Partridge TA,Hathout Y,Nagaraju K

Fri Dec 17 00:00:00 EST 2021

Momordica charantia polysaccharides modulate the differentiation of neural stem cells via SIRT1/Β-catenin axis in cerebral ischemia/reperfusion.

Stem cell research & therapy

Hu Z,Li F,Zhou X,Zhang F,Huang L,Gu B,Shen J,Qi S

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunohistochemistry

Mon Nov 16 00:00:00 EST 2020

Momordica charantia polysaccharides modulate the differentiation of neural stem cells via SIRT1/Β-catenin axis in cerebral ischemia/reperfusion.

Stem cell research & therapy

Hu Z,Li F,Zhou X,Zhang F,Huang L,Gu B,Shen J,Qi S

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunohistochemistry

Mon Nov 16 00:00:00 EST 2020

TGF-β-driven muscle degeneration and failed regeneration underlie disease onset in a DMD mouse model.

JCI insight

Mázala DA,Novak JS,Hogarth MW,Nearing M,Adusumalli P,Tully CB,Habib NF,Gordish-Dressman H,Chen YW,Jaiswal JK,Partridge TA

B35138 was used in Immunohistochemistry-immunofluorescence to provide in vivo evidence of early-stage deficit in regenerative myogenesis in D2-mdx mice and implicates TGF-β as a major component of a pathogenic positive feedback loop in this model, identifying this feedback loop as a therapeutic target.

Thu Mar 26 00:00:00 EDT 2020

Pharmacologic ATM but not ATR kinase inhibition abrogates p21-dependent G1 arrest and promotes gastrointestinal syndrome after total body irradiation.

Scientific reports

Vendetti FP,Leibowitz BJ,Barnes J,Schamus S,Kiesel BF,Abberbock S,Conrads T,Clump DA,Cadogan E,O'Connor MJ,Yu J,Beumer JH,Bakkenist CJ

B35138 was used in immunohistochemistry - paraffin section to discover that ATM kinase activity is essential for p21-dependent and p21-independent mechanisms that radioprotect intestinal crypts

Wed Feb 01 00:00:00 EST 2017

Context-Dependent Effects of Amplified MAPK Signaling during Lung Adenocarcinoma Initiation and Progression.

Cell reports

Cicchini M,Buza EL,Sagal KM,Gudiel AA,Durham AC,Feldser DM

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunofluorescence

Tue Feb 21 00:00:00 EST 2017

Myoblasts and macrophages are required for therapeutic morpholino antisense oligonucleotide delivery to dystrophic muscle.

Nature communications

Novak JS,Hogarth MW,Boehler JF,Nearing M,Vila MC,Heredia R,Fiorillo AA,Zhang A,Hathout Y,Hoffman EP,Jaiswal JK,Nagaraju K,Cirak S,Partridge TA

B35138 was used in Immunohistochemistry to show that robust morpholino (PMO) uptake and efficient production of dystrophin following PMO administration coincide with areas of myofiber regeneration and inflammation.

Mon Oct 16 00:00:00 EDT 2017

Chronic Exposure to the Fusarium Mycotoxin Deoxynivalenol: Impact on Performance, Immune Organ, and Intestinal Integrity of Slow-Growing Chickens.

Toxins

Chen SS,Li YH,Lin MF

B35138 was used in Immunohistochemistry to investigate the long-term effects of deoxynivalenol consumption on avian growth performance, on the proliferation, apoptosis, and DNA damage of spleen cells, and on intestinal integrity.

Fri Oct 20 00:00:00 EDT 2017

Effect of genetic background on the dystrophic phenotype in mdx mice.

Human molecular genetics

Coley WD,Bogdanik L,Vila MC,Yu Q,Van Der Meulen JH,Rayavarapu S,Novak JS,Nearing M,Quinn JL,Saunders A,Dolan C,Andrews W,Lammert C,Austin A,Partridge TA,Cox GA,Lutz C,Nagaraju K

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunofluorescence

Fri Jan 01 00:00:00 EST 2016

Muscular dystrophy in the mdx mouse is a severe myopathy compounded by hypotrophy, hypertrophy and hyperplasia.

Skeletal muscle

Duddy W,Duguez S,Johnston H,Cohen TV,Phadke A,Gordish-Dressman H,Nagaraju K,Gnocchi V,Low S,Partridge T

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunofluorescence

Tue May 19 00:00:00 EDT 2015

Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regeneration of Injured Muscles.

Scientific reports

Leikina E,Defour A,Melikov K,Van der Meulen JH,Nagaraju K,Bhuvanendran S,Gebert C,Pfeifer K,Chernomordik LV,Jaiswal JK

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunofluorescence

Tue Dec 15 00:00:00 EST 2015

Delta-9-tetrahydrocannabinol disrupts hippocampal neuroplasticity and neurogenesis in trained, but not untrained adolescent Sprague-Dawley rats.

Brain research

Steel RW,Miller JH,Sim DA,Day DJ

B35138 was used in immunohistochemistry - paraffin section to identify effects of Delta(9)-tetrahydrocannabinol on the early induction of adaptive processes that support learning

Sat Feb 22 00:00:00 EST 2014

p27 is regulated independently of Skp2 in the absence of Cdk2.

Biochimica et biophysica acta

Kotoshiba S,Gopinathan L,Pfeiffenberger E,Rahim A,Vardy LA,Nakayama K,Nakayama KI,Kaldis P

Published figure using BrdU monoclonal antibody (Product # B35138) in Immunohistochemistry

Sat Feb 01 00:00:00 EST 2014

A novel transgenic mouse model of growth plate dysplasia reveals that decreased chondrocyte proliferation due to chronic ER stress is a key factor in reduced bone growth.

Disease models & mechanisms

Gualeni B,Rajpar MH,Kellogg A,Bell PA,Arvan P,Boot-Handford RP,Briggs MD

B35138 was used in immunohistochemistry - paraffin section to elucidate decreased chondrocyte proliferation caused by chronic ER stress as a key factor in reduced bone growth in a novel transgenic mouse model of growth plate dysplasia

Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (MoBU-1), Biotin

BrdU Antibody (B35138) in IHC

Product Details

B35138

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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