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B7-H3 (CD276) Antibody (MA5-47649) in ICC/IF
Immunocytochemistry analysis of B7-H3 (CD276) in aldehyde fixed human MCF-7 breast ductal carcinoma cells. The cells were labeled with mouse B7-H3 (CD276) monoclonal antibody (Product # MA5-47649). The antibody was detected using goat anti-mouse DyLight® 594.
Product Details
MA5-47649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M045
Immunogen
Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2942630
Product Specific Information
Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Target Information
CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4Ig-B7-H3; B7 homolog 3; B7-H3; CD276; CD276 antigen; Costimulatory molecule; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276
Gene Aliases: 4Ig-B7-H3; B7-H3; B7H3; B7RP-2; CD276; PSEC0249; UNQ309/PRO352
UniProt ID: (Human) Q5ZPR3
Entrez Gene ID: (Human) 80381
immune response
cell proliferation
positive regulation of bone mineralization
positive regulation of T cell proliferation
T cell activation
negative regulation of T cell proliferation
negative regulation of interferon-gamma biosynthetic process
positive regulation of interferon-gamma biosynthetic process
negative regulation of interleukin-2 biosynthetic process
positive regulation of osteoblast differentiation
negative regulation of inflammatory response
regulation of immune response
positive regulation of interleukin-2 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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