Invitrogen

BAD Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (48) BAD antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-85624

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bad.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792764

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Rhesus Monkey (95%), Chimpanzee (100%).

Positive Control: 293T, A431, HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; BCL-X/BCL-2 binding protein; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; BCL2-associated agonist of cell death; BCL2-binding component 6; BCL2-binding protein; Bcl2-L-8; OTTMUSP00000017561; OTTMUSP00000022400

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Gene Aliases: BAD; BBC2; BBC6; BCL2L8

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UniProt ID: (Human) Q92934

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Entrez Gene ID: (Human) 572

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Function(s)
protein binding phospholipid binding lipid binding cysteine-type endopeptidase activator activity involved in apoptotic process protein kinase binding protein phosphatase 2B binding protein kinase B binding protein heterodimerization activity 14-3-3 protein binding
Process(es)
release of cytochrome c from mitochondria protein insertion into mitochondrial membrane involved in apoptotic signaling pathway glucose catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process spermatogenesis extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to glucose positive regulation of autophagy positive regulation of mitochondrial membrane potential suppression by virus of host apoptotic process cytokine-mediated signaling pathway cerebral cortex development positive regulation of insulin secretion response to estradiol response to progesterone positive regulation of glucokinase activity response to testosterone response to oleic acid positive regulation of insulin secretion involved in cellular response to glucose stimulus response to drug response to hydrogen peroxide glucose homeostasis positive regulation of apoptotic process response to amino acid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process type B pancreatic cell proliferation response to ethanol positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of proteolysis negative regulation of cytolysis ADP metabolic process ATP metabolic process regulation of mitochondrial membrane permeability pore complex assembly positive regulation of epithelial cell proliferation response to glucocorticoid response to calcium ion positive regulation of apoptotic process by virus cellular process regulating host cell cycle in response to virus cellular response to chromate cellular response to mechanical stimulus cellular response to nicotine cellular response to lipid cellular response to hypoxia positive regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand intrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway positive regulation of neuron death positive regulation of type B pancreatic cell development positive regulation of intrinsic apoptotic signaling pathway
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