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BAFF Antibody (500-P163GBT-25UG) in ELISA
Sandwich ELISA: To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of BAFF Polyclonal Antibody, Biotin (Product # 500-P163GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech BAFF Polyclonal Antibody (Product # 500-P163G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBAFF.
Product Details
500-P163GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human BAFF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929579
Product Specific Information
AA Sequence of recombinant protein: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBAFF.
Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P163GBT-1MG will be provided as 2 x 500 µg
Target Information
BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4
Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4
UniProt ID: (Human) Q9Y275
Entrez Gene ID: (Human) 10673
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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