Invitrogen

BAMBI Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

7 References

View all (18) BAMBI antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

BAMBI Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

BAMBI Antibody (PA5-38027) in IHC (P)

Immunohistochemistry of BAMBI in human lung tissue with BAMBI Polyclonal Antibody (Product # PA5-38027) at 5 µg/mL.

BAMBI Antibody in Immunocytochemistry (ICC/IF)

BAMBI Antibody in Immunohistochemistry (IHC)

Product Image Gallery

BAMBI Antibody — Figure 5 miR-106a can target BMP and activin membrane-bound inhibitor (BAMBI) instead of cyclin-dependent kinase inhibitor 1A ( p21) during adipogenic differentiation. ( A ) The target site of miR-106a within the porcine BAMBI mRNA 3'UTR and mutational site of BAMBI 3'UTR; ( B ) the dual-luciferase assay was performed by com-transfection of the miR-106a agomir and wild-type vectors or mutant vectors. Relative luciferase activity was represented by Renilla luciferase/firefly luciferase (RLUC/FLUC); ( C ) the relative BAMBI mRNA expression levels after treatment with an miR-106a agomir; ( D ) western blot analysis of p21 and BAMBI protein expression after treatment with an miR-106a agomir; ( E ) the quantification of p21 and BAMBI protein expression levels. Data are representative of the mean +- standard deviation of three independent experiments. * p < 0.05; ** p < 0.01. {KD}

Product Details

PA5-38027

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

17 amino acid peptide near the center of human BAMBI

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554631

Product Specific Information

A recommended positive control is mouse lung tissue lysate.

Target Information

BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development. BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice.

Current research in physiology

Wooten JS,Poole KE,Harris MP,Guilford BL,Schaller ML,Umbaugh D,Seija A

PA5-38027 was used in Western Blot to determine the effect of voluntary wheel running (VWR) during weight-loss on hepatic lipid and inflammatory biomarkers using a murine model.

Fri Feb 11 00:00:00 EST 2022

The effects of voluntary wheel running during weight-loss on biomarkers of hepatic lipid metabolism and inflammation in C57Bl/6J mice.

Current research in physiology

Wooten JS,Poole KE,Harris MP,Guilford BL,Schaller ML,Umbaugh D,Seija A

PA5-38027 was used in Western Blot to determine the effect of voluntary wheel running (VWR) during weight-loss on hepatic lipid and inflammatory biomarkers using a murine model.

Fri Feb 11 00:00:00 EST 2022

Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production.

The Journal of biological chemistry

Chen X,Zhao C,Xu Y,Huang K,Wang Y,Wang X,Zhou X,Pang W,Yang G,Yu T

Published figure using BAMBI polyclonal antibody (Product # PA5-38027) in Western Blot

Fri Aug 27 00:00:00 EDT 2021

Adipose-specific BMP and activin membrane-bound inhibitor (BAMBI) deletion promotes adipogenesis by accelerating ROS production.

The Journal of biological chemistry

Chen X,Zhao C,Xu Y,Huang K,Wang Y,Wang X,Zhou X,Pang W,Yang G,Yu T

Published figure using BAMBI polyclonal antibody (Product # PA5-38027) in Western Blot

Fri Aug 27 00:00:00 EDT 2021

BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers.

Biomolecules

López-Pérez Ó,Bernal-Martín M,Hernaiz A,Llorens F,Betancor M,Otero A,Toivonen JM,Zaragoza P,Zerr I,Badiola JJ,Bolea R,Martín-Burriel I

PA5-38027 was used in Immunohistochemistry (Paraffin) to confirm the role of BAMBI and CHGA in prion neuropathology in vivo, but besides prion replication, they seem to be involved in the characteristic neuroinflammatory response associated to prion infection.

Sat May 02 00:00:00 EDT 2020

BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers.

Biomolecules

López-Pérez Ó,Bernal-Martín M,Hernaiz A,Llorens F,Betancor M,Otero A,Toivonen JM,Zaragoza P,Zerr I,Badiola JJ,Bolea R,Martín-Burriel I

Sat May 02 00:00:00 EDT 2020

Upregulated microRNA-106a Promotes Porcine Preadipocyte Proliferation and Differentiation by Targeting Different Genes.

Genes

Huang K,Shi X,Wang J,Yao Y,Peng Y,Chen X,Li X,Yang G

Published figure using BAMBI polyclonal antibody (Product # PA5-38027) in Western Blot

Mon Oct 14 00:00:00 EDT 2019

Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters.

Molecular cell

Grunseich C,Wang IX,Watts JA,Burdick JT,Guber RD,Zhu Z,Bruzel A,Lanman T,Chen K,Schindler AB,Edwards N,Ray-Chaudhury A,Yao J,Lehky T,Piszczek G,Crain B,Fischbeck KH,Cheung VG

PA5-38027 was used in Immunohistochemistry-immunofluorescence to study how R-loops promote transcription in motor neuron disease.

Thu Feb 01 00:00:00 EST 2018

Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene Promoters.

Molecular cell

Grunseich C,Wang IX,Watts JA,Burdick JT,Guber RD,Zhu Z,Bruzel A,Lanman T,Chen K,Schindler AB,Edwards N,Ray-Chaudhury A,Yao J,Lehky T,Piszczek G,Crain B,Fischbeck KH,Cheung VG

PA5-38027 was used in Immunohistochemistry-immunofluorescence to study how R-loops promote transcription in motor neuron disease.

Thu Feb 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: BMP and activin membrane-bound inhibitor homolog; BMP and activin membrane-bound inhibitor, homolog; Kinase-deficient TGFbeta superfamily receptor subunit; Non-metastatic gene A protein; Putative transmembrane protein NMA

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Gene Aliases: 2610003H06Rik; BAMBI; NMA

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UniProt ID: (Human) Q13145, (Rat) Q91XN4, (Mouse) Q9D0L6

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Entrez Gene ID: (Human) 25805, (Rat) 83837, (Mouse) 68010

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Function(s)

frizzled binding type II transforming growth factor beta receptor binding

Process(es)

transforming growth factor beta receptor signaling pathway positive regulation of cell proliferation regulation of cell shape positive regulation of epithelial to mesenchymal transition cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein binding positive regulation of catenin import into nucleus positive regulation of transcription, DNA-templated positive regulation of canonical Wnt signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BAMBI Polyclonal Antibody

BAMBI Antibody (PA5-38027) in IHC (P)

Product Details

PA5-38027

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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