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BCL-3 Antibody (PA5-112367) in ICC/IF
Immunocytochemichal analysis of BCL-3 in A549 cells using a Polyclonal antibody (Product # PA5-112367) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human B-cell lymphoma 3 protein (362-454AA)
Conjugate
Form
Liquid
Concentration
3.4 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2867103
Target Information
B cell Lymphoma 3 is a member of the IkB subfamily of inhibitors. This subfamily is part of the NF-kB transcription factor protein family and it is suggested that a balance in the concentration of various NF-kB family members regulates apoptosis and survival of activated T cells. Bcl-3 has been shown to have versatile functions such as cytoplasmic activation of p50 homodimers, translocation to the nucleus, and modulation of the transcriptional machinery in the nucleus. Bcl-3 activity often relies on several nuclear interacting proteins such as Tip60, Jab1, Bard1, and Pirin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell CLL/lymphoma 3; B-cell leukemia/lymphoma 3; B-cell lymphoma 3 protein; B-cell lymphoma 3 protein homolog; B-cell lymphoma 3-encoded protein; BCL-3; Bcl-3 protein; chronic lymphatic leukemia protein; Proto-oncogene BCL3
Gene Aliases: AI528691; Bcl-3; BCL3; BCL4; D19S37
UniProt ID: (Human) P20749, (Mouse) Q9Z2F6
Entrez Gene ID: (Human) 602, (Mouse) 12051
protein import into nucleus, translocation
follicular dendritic cell differentiation
marginal zone B cell differentiation
humoral immune response mediated by circulating immunoglobulin
germinal center formation
transcription, DNA-templated
cellular response to DNA damage stimulus
I-kappaB kinase/NF-kappaB signaling
response to virus
response to UV-C
antimicrobial humoral response
extracellular matrix organization
DNA damage response, signal transduction by p53 class mediator
positive regulation of interferon-gamma production
T-helper 1 type immune response
regulation of NF-kappaB import into nucleus
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
defense response to protozoan
regulation of apoptotic process
negative regulation of apoptotic process
T-helper 2 cell differentiation
positive regulation of interleukin-10 biosynthetic process
negative regulation of interleukin-8 biosynthetic process
positive regulation of translation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
spleen development
regulation of DNA binding
maintenance of protein location in nucleus
regulation of transcription, DNA-templated
Performance Guarantee
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