Proteintech

BCL2 Polyclonal Antibody

2,131 References
View all (186) BCL-2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

12789-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:4,000
View 1,946 publications 1,946 publications

Immunohistochemistry (IHC)

-
View 99 publications 99 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:300
-

Immunocytochemistry (ICC/IF)

-
View 33 publications 33 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells
View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg
View 8 publications 8 publications

ChIP assay (ChIP)

Assay-dependent
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 16 publications 16 publications

Not applicable (N/A)

-
View 27 publications 27 publications
Product Specifications

Species Reactivity

Human

Published species

Bovine, Chicken, Fish, Guinea pig, Human, Mouse, Pig, Plant, Rabbit, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

BCL2 Fusion Protein Ag3508 (1-239 aa encoded by BC027258)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is not suitable for rat and mouse species.

Immunogen sequence: MAHAGRTGY DNREIVMKYI HYKLSQRGYE WDAGDVGAAP PGAAPAPGIF SSQPGHTPHP AASRDPVART SPLQTPAAPG AAAGPALSPV PPVVHLTLRQ AGDDFSRRYR RDFAEMSSQL HLTPFTARGR FATVVEELFR DGVNWGRIVA FFEFGGVMCV ESVNREMSPL VDNIALWMTE YLNRHLHTWI QDNGGWDAFV ELYGPSMRPL FDFSWLSLKT LLSLALVGAC ITLGAYLGHK (1-239 aa encoded by BC027258)

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl 2; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: Bcl-2; BCL2; PPP1R50

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UniProt ID: (Human) P10415

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Entrez Gene ID: (Human) 596

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Function(s)
protease binding protein binding transcription factor binding channel activity channel inhibitor activity ubiquitin protein ligase binding identical protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding
Process(es)
protein polyubiquitination ossification ovarian follicle development metanephros development branching involved in ureteric bud morphogenesis behavioral fear response B cell homeostasis release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphoid progenitor cell differentiation B cell lineage commitment response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization apoptotic process humoral immune response cellular response to DNA damage stimulus actin filament organization axonogenesis female pregnancy cell aging male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress response to radiation response to toxic substance post-embryonic development response to iron ion response to UV-B response to gamma radiation regulation of gene expression negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation positive regulation of smooth muscle cell migration cell growth peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis regulation of transmembrane transporter activity negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment regulation of protein homodimerization activity regulation of protein heterodimerization activity negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly spleen development thymus development digestive tract morphogenesis oocyte development positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to organic substance cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway negative regulation of G1/S transition of mitotic cell cycle negative regulation of reactive oxygen species metabolic process negative regulation of anoikis negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway
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