Invitrogen

BDNF Recombinant Superclonal Antibody (19HCLC)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

710306

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

19HCLC

Immunogen

Recombinant protein corresponding to amino acids 129-247 of human BDNF
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532677

Product Specific Information

This antibody is predicted to react with non-human primate, rat and rabbit based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Abrineurin; anorexia BDNF; BDNF; Brain-derived neurotrophic factor; H-BDNF; neurotrophin

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Gene Aliases: ANON2; BDNF; BULN2

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UniProt ID: (Human) Q9BYY7, (Mouse) P21237

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Entrez Gene ID: (Human) 627, (Mouse) 12064

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Function(s)
neurotrophin TRKB receptor binding growth factor activity receptor binding protein binding neurotrophic factor
Process(es)
cell-cell signaling nervous system development axon guidance synapse assembly brain-derived neurotrophic factor receptor signaling pathway positive regulation of brain-derived neurotrophic factor receptor signaling pathway negative regulation of neuron apoptotic process collateral sprouting positive regulation of collateral sprouting positive regulation of synapse assembly ureteric bud development behavioral fear response mitochondrial electron transport, NADH to ubiquinone transmembrane receptor protein tyrosine kinase signaling pathway gamma-aminobutyric acid signaling pathway negative regulation of neuroblast proliferation axon target recognition learning or memory learning feeding behavior neuron recognition glutamate secretion dendrite development regulation of metabolic process nerve development regulation of axon extension negative regulation of synaptic transmission, GABAergic positive regulation of peptidyl-serine phosphorylation mechanoreceptor differentiation response to drug fear response negative regulation of apoptotic process regulation of neuron apoptotic process positive regulation of neuron differentiation negative regulation of striated muscle tissue development regulation of retinal cell programmed cell death regulation of synaptic plasticity regulation of long-term neuronal synaptic plasticity positive regulation of long-term neuronal synaptic plasticity regulation of short-term neuronal synaptic plasticity axon extension neuron projection morphogenesis inner ear development positive regulation of sequence-specific DNA binding transcription factor activity excitatory postsynaptic potential inhibitory postsynaptic potential negative regulation of cell death taste bud development cellular response to acid chemical dendrite extension negative regulation of neuron death neuron projection extension positive regulation of glucocorticoid receptor signaling pathway
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